Abstract
The work describes the development of a portable and autonomous biosensing label free platform for detection of biotoxin substances. The biosensor is realized as an on-chip package-free micro electrochemical cell consisting of a counter electrode (CE), a reference electrode (RE) and a working electrode (WE) patterned on a single silicon chip. To improve sensor sensitivity, the WE was implemented as a microelectrode array of 40 micron diameter gold disks with 400 micron centre-to-centre distance, which underwent corresponding surface modification for antibody immobilisation. The interfacial biosensor changes produced by T2-mycotoxin antigen-antibody reaction were sensed by means of Electrochemical Impedance Spectroscopy in a frequency range from 10 Hz to 100 kHz. The signal processing algorithm for mycotoxin quantification was based on analysis of biosensor impedance spectra and its equivalent electrical circuit. It is implemented in corresponding software at microcontroller and single-board computer level in such a manner that the results can be produced at point-of-need or in the decision center without user intervention. The instrumentation represented a mix signal measurement system which consisted of analog and digital parts. The analog part constituted a low noise, highly-sensitive hardware which implemented impedance measurements on the basis of a quadrature signal processing of the biosensor in response to a harmonic stimulation signal. The key unit of the digital part of the device was an Atmel microcontroller with inbuilt 12-bit ADC and external 16-bit DAC, which are responsible for device configuration, stimulation signal generation, biosensor signal digitalization, its initial signal processing and communication with the single-board computer. A calibration of the platform in the range of 0–250 ppm of T2 toxin concentrations confirmed that the system can provide successful detection of the toxin at the levels above 25 ppm.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Shriver-Lake, L.C., Ligler, F.S.: The array biosensor for counterterrorism. IEEE Sens. J. 5(4), 751–756 (2005)
Ahmad, A., Moore, E.J.: Comparison of cell-based biosensors with traditional analytical techniques for cytotoxicity monitoring and screening of polycyclic aromatic hydrocarbons in the environment. Anal. Lett. 42(1), 1–28 (2009)
Brennan, D., Lambkin, P., Moore, E.J., Galvin, P.: An integrated optofluidic platform for DNA hybridization and detection. IEEE Sens. J. 8(5), 536–542 (2008)
Bryan, T., Luo, X., Bueno, P.R., Davis, J.J.: An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood. Biosens. Bioelectron. 39(1), 94–98 (2013)
Zhang, W., Du, Y., Wang, M.L.: Noninvasive glucose monitoring using saliva nano-biosensor. Sens. Bio-Sens. Res. 4, 23–29 (2015)
Wang, X., Lu, X., Chen, J.: Development of biosensor technologies for analysis of environmental contaminants. Trends Environ. Anal. Chem. 2, 25–32 (2014)
Yong, D., Liu, C., Zhu, C., Yu, D., Liu, L., Zhai, J., Dong, S.: Detecting total toxicity in water using a mediated biosensor system with flow injection. Chemosphere 139, 109–116 (2015)
Herzog, G., Moujahid, W., Twomey, K., Lyons, C., Ogurtsov, V.I.: On-chip electrochemical microsystems for measurements of copper and conductivity in artificial seawater. Talanta 116, 26–32 (2013)
Ogurtsov, V.I., Twomey, K., Herzog, G.: Development of an electrochemical sensing system for environmental monitoring of port water quality to integrate on-board an autonomous robotic fish. In: Hashmi, S. (ed.) Comprehensive Materials Processing. Sensor Materials, Technologies and Applications, vol. 13, pp. 317–351. Elsevier Science, Oxford (2014)
Said, N.A.M., Twomey, K., Ogurtsov, V.I., Arrigan, D.W.M., Herzog, G.: Fabrication and electrochemical characterization of micro- and nanoelectrode arrays for sensor applications. J. Phys. Conf. Ser. 307, 012052 (2011)
Thevenot, D.R., Toth, K., Durst, R.A., Wilson, G.S.: Electrochemical biosensors: recommended definitions and classification. Pure Appl. Chem. 71, 2333–2348 (1999)
Scheller, F.W., Wollenbergera, U., Warsinkea, A., Lisdata, F.: Research and development in biosensors. Curr. Opin. Biotechnol. 12, 35–40 (2001)
Vo-Dinh, T., Cullum, B.: Biosensors and biochips: advances in biological and medical diagnostics. Fresen. J. Anal. Chem. 366(6–7), 540–551 (2000)
Hock, B., Seifert, M., Kramer, K.: Engineering receptors and antibodies for biosensors. Biosens. Bioelectron. 17(3), 239–249 (2002)
Wollenberger, U.: Third generation biosensors - integrating recognition and transduction in electrochemical sensors. In: Gorton, L. (ed.) Biosensors and Modem Biospecific Analytical Techniques, pp. 65–130. Elsevier, Amsterdam (2005)
Koyun, A., Ahlatcıoğlu, E., İpek, Y.K.: Biosensors and their principles. In: Kara, S. (ed.) A Roadmap of Biomedical Engineers and Milestones. INTECH, Rijeka (2012)
Sharma, S., Byrne, H., O’Kennedy, R.J.: Antibodies and antibody-derived analytical biosensors. Essays Biochem. 60(1), 9–18 (2016)
Byrne, B., Stack, E., Gilmartin, N., O’Kennedy, R.: Antibody-based sensors: principles, problems and potential for detection of pathogens and associated toxins. Sensors 9(6), 4407–4445 (2009)
Perumal, V., Hashim, U.: Advances in biosensors: principle, architecture and applications. J. Appl. Biomed. 12(1), 1–15 (2014)
Dodeigne, C., Thunus, L., Lejeune, R.: Chemiluminescence as diagnostic tool. A review. Talanta 51, 415–439 (2000)
Jiang, X., Li, D., Xu, X., Ying, Y., Li, Y., Ye, Z., et al.: Immunosensors for detection of pesticide residues. Biosens. Bioelectron. 23(11), 1577–1587 (2008)
Yalow, R.S., Berson, S.A.: Assay of plasma insulin in human subjects by immunological methods. Nature 184, 1648–1649 (1959)
Patel, Pd.: (Bio)sensors for measurement of analytes implicated in food safety. A review. Trends Anal. Chem. 21(2), 96–115 (2002)
Ogurtsov, V.I., Twomey, K., Pulka, J.: A portable sensing system for impedance based detection of biotoxin substances. In: 10th International Joint Conference on Biomedical Engineering Systems and Technologies Proceedings, BIODEVICES, (BIOSTEC 2017), Porto, Portugal, vol. 1, pp. 54–62 (2017)
Sadana, A.: Biosensors: Kinetics of Binding and Dissociation Using Fractals, 1st edn. Elsevier, Amsterdam (2003)
Zhang, S., Zhao, H., John, R.: Development of a generic microelectrode array biosensing system. Anal. Chimica Acta 421(2), 175–187 (2000)
Willner, I., Katz, E., Willner, B.: Layered functionalized electrodes for electrochemical biosensor applications. In: Yang, V.C., Ngo, T.T. (eds.) Biosensors and Their Applications. Elsevier, Amsterdam (2000)
Arrigan, D.W.M.: Electrochemical Strategies in Detection Science. Royal Society of Chemistry, Cambridge (2016)
Twomey, K., O’Mara, P., Pulka, J., McGillycuddy, S., et al.: Fabrication and characterisation of a test platform integrating nanoporous structures with biochemical functionality. IEEE Sens. J. 15(8), 4329–4337 (2015)
Kafi, A.K.M., Lee, D.-Y., Park, S.-H., Kwon, Y.-S.: Potential application of hemoglobin as an alternative to peroxidase in a phenol biosensor. Thin Solid Films 516(9), 2816–2821 (2008)
Zhou, Y., Tang, L., Zeng, G., Zhang, C., Xie, X., Liu, Y., Wang, J., Tang, J., Zhang, Y., Deng, Y.: Label free detection of lead using impedimetric sensor based on ordered mesoporous carbon–gold nanoparticles and DNAzyme catalytic beacons. Talanta 146, 641–647 (2016)
Rushworth, J.V., Ahmed, A., Griffiths, H.H., Pollock, N.M., Hooper, N.M., Millner, P.A.: A label-free electrical impedimetric biosensor for the specific detection of Alzheimer’s amyloid-beta oligomers. Biosens. Bioelectron. 56, 83–90 (2014)
Arrigan, D.W.M.: Nanoelectrodes, nanoelectrode arrays and their applications. Analyst 129(12), 1157–1165 (2004)
Bennett, J.W., Klich, M.: Mycotoxins. Clin. Microbiol. Rev. 16(3), 497–516 (2003)
Gupta, R.C. (ed.): Handbook of Toxicology of Chemical Warfare Agents, 1st edn. Elsevier, Amsterdam (2009)
Ler, S.G., Lee, F.K., Gopalakrishnakone, P.: Trends in detection of warfare agents detection methods for ricin, staphylococcal enterotoxin B and T-2 toxin. J. Chromatogr. A 1133, 1–12 (2006)
Zheng, M.Z., Richard, J.L., Binder, J.: A review of rapid methods for the analysis of mycotoxins. Mycopathologia 161, 261–273 (2006)
Pittet, A.: Keeping the mycotoxins out: experience gathered by an international food company. Nat. Toxins 3(4), 281–287 (1995)
De Saeger, S., Van Peteghem, C.: Dipstick enzyme immunoassay to detect Fusarium T-2 toxin in wheat. Appl. Environ. Microbiol. 62(6), 1880–1884 (1996)
Said, M., Azura, N.: Electrochemical biosensor based on microfabricated electrode arrays for life sciences applications. Ph.D. thesis, University College Cork (2014)
Britz, D., Strutwolf, J.: Digital Simulation in Electrochemistry, 4th edn. Springer, Cham (2016). https://doi.org/10.1007/978-3-319-30292-8
Hermes, M., Scholz, F.: Solid-state electrochemical reactions of electroactive microparticles and nanoparticles in a liquid electrolyte environment. In: Kharton, V.V. (ed.) Solid State Electrochemistry I: Fundamentals, Materials and Their Applications. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim (2009)
Guo, J., Lindner, E.: Cyclic voltammograms at coplanar and shallow recessed microdisc electrode arrays: guidelines for design and experiment. Anal. Chem. 81(1), 130–138 (2009)
Said, N.A.M., Twomey, K., Herzog, G., Ogurtsov V.I.: Fabrication and characterization of microfabricated on-chip microelectrochemical cell for biosensing applications. In: Zaaba, S.K., Zakaria, S.M.M.S., Kamarudin, K., et al. (eds.) Asian Conference on Chemical Sensors 2015, ACCS 2015, vol. 1808, pp. 020032-1–020032-13. AIP, Melville (2017)
Masson, J.-F., Battaglia, T.M., Davidson, M.J., Kim, Y.-C., Prakash, A.M.C., Beaudoin, S., Booksh, K.S.: Biocompatible polymers for antibody support on gold surfaces. Talanta 67(5), 918–925 (2005)
Haddada, M.B., Huebner, M., Casale, S., Knopp, D., Niessner, R., Salmain, M., Boujday, S.: Gold nanoparticles assembly on silicon and gold surfaces: mechanism, stability, and efficiency in diclofenac biosensing. J. Phys. Chem. C 120(51), 29302–29311 (2016)
Vashist, S.K., Dixit, C.K., MacCraith, B.D., O’Kennedy, R.: Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay. Analyst 136(21), 4431–4436 (2011)
Raj, J., Herzog, G., Manning, M., Volcke, C., Maccraith, B., Ballantyne, S., Thompson, M., Arrigan, D.: Surface immobilisation of antibody on cyclic olefin copolymer for sandwich immunoassay. Biosens. Bioelectron. 24, 2654–2658 (2009)
Barsoukov, E., Macdonald, J.R. (eds.): Impedance Spectroscopy Theory, Experiment, and Applications, 2nd edn. Wiley, Hoboken (2005)
Lasia, A.: Electrochemical impedance spectroscopy and its applications. In: Conway, B.E., Bockris, J., White, R.E. (eds.) Modern Aspects of Electrochemistry, vol. 32, pp. 143–248. Kluwer Academic/Plenum Publishers, New York (1999)
Suni, I.I.: Impedance methods for electrochemical sensors using nanomaterials. Trends Anal. Chem. 27(7), 604–610 (2008)
Jacobsen, T., West, K.: Diffusion impedance in planar, cylindrical and spherical symmetry. Elecrochimika Acta 40(2), 255–262 (1995)
Hu, S.Q., Wu, Z.Y., Zhou, Y.M., Cao, Z.X., Shen, G.L., Yu, R.Q.: Capacitive immunosensor for transferrin based on an o-aminobenzenthiol oligomer layer. Anal. Chim. Acta 458, 297–304 (2002)
Acknowledgements
Financial support of this work by European Commission projects FP7-SEC-2011.3.4-2 “HANDHOLD: HANDHeld OLfactory Detector” and H2020-NMP-29-2015 “HISENTS: High level Integrated SEnsor for NanoToxicity Screening” is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this paper
Cite this paper
Ogurtsov, V.I., Twomey, K. (2018). A Portable Chemical Detection System with Anti-body Biosensor for Impedance Based Monitoring of T2-mycotoxin Bioterrorism Agents. In: Peixoto, N., Silveira, M., Ali, H., Maciel, C., van den Broek, E. (eds) Biomedical Engineering Systems and Technologies. BIOSTEC 2017. Communications in Computer and Information Science, vol 881. Springer, Cham. https://doi.org/10.1007/978-3-319-94806-5_3
Download citation
DOI: https://doi.org/10.1007/978-3-319-94806-5_3
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-94805-8
Online ISBN: 978-3-319-94806-5
eBook Packages: Computer ScienceComputer Science (R0)