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Bioethanol Production Using Saccharomyces cerevisiae Immobilized in Calcium Alginate–Magnetite Beads and Application of Response Surface Methodology to Optimize Bioethanol Yield

  • Snehal Ingale
  • Venkata Anand Parnandi
  • Sanket J. JoshiEmail author
Chapter
Part of the Biofuel and Biorefinery Technologies book series (BBT, volume 7)

Abstract

We studied the bioethanol production in molasses-based medium by yeast Saccharomyces cerevisiae immobilized in calcium alginate magnetite beads (CAMB). The yeast was isolated from soil samples collected near a local sugar mill, and identified as S. cerevisiae. We synthesized magnetite nanoparticles and immobilized yeast in CAMB. The media components and environmental parameters were statistically screened and optimized for better ethanol production, using statistical design methodologies—factorial designs and response surface methodology. The factors of molasses concentration, temperature and incubation time were found to have significant effect on ethanol production. The immobilized cells could be reused for more than 120 days, retaining its original activity. The CAMBs with immobilized yeast cells were analysed by ESEM with EDAX, after 96 h of fermentation to observe the surface structure of the beads. It can be observed that yeast was immobilized in the beads and actively growing. Further ethanol production was carried out in packed-bed column reactor using yeast immobilized in CAMB, under fed-batch mode. The average ethanol produced by fed-batch fermentation was 1.832 g% ± 0.103, and the average ethanol yield was 81.420% ± 4.6. Further studies using yeast immobilized in CAMB are recommended to carry out continuous fermentation, and further scale up bioethanol production in a magnetically stabilized fluidized bed reactor (MSFBR), where the position of the beads in the system can be controlled and maintained by the application of oscillating electric field.

Notes

Acknowledgements

SI and VP would like to thank ARIBAS and CVM, and SJ would like to acknowledge Sultan Qaboos University for providing the research facility.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2019

Authors and Affiliations

  • Snehal Ingale
    • 1
  • Venkata Anand Parnandi
    • 1
  • Sanket J. Joshi
    • 2
    Email author
  1. 1.Ashok and Rita Patel Institute of Integrated Study& Research in Biotechnology and Allied Sciences (ARIBAS)Sardar Patel UniversityAnandIndia
  2. 2.Central Analytical and Applied Research Unit, College of ScienceSultan Qaboos UniversityMuscatOman

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