Abstract
Biological agents like pathogenic bacteria represent a major threat to the public if spread. Bacteria may replicate in their host and can spread in an unpredictable way. Standoff detection based on laser-induced fluorescence may help to mitigate the associated risks. Nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and tryptophan are among others the most important fluorophores in bacteria. Bacteria adapt to their environment, and thus the same bacterial species may be composed of different components and relative concentrations of them depending on environmental conditions. Fluorescence spectra of a Bacillus thuringiensis population were compared during different growth phases. Laser pulses with two different excitation wavelengths, 280 and 355 nm, were used. For 280 nm excitation, the measured spectra show a difference in spectral features between the bacterial population before sporulation and after it was partially sporulated. In comparison, a smaller variation in 355 nm excited LIF spectra of a bacterial population during exponential growth and the aging population is observed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Buteau, S., Rowsell, S.: Biological material detection identification and monitoring: combining point and standoff sensors technologies. Proc. SPIE. 9995, 99950B–10 (2016)
de Graef, M.R., Alexeeva, S., Snoep, J.L., de Mattos, M.J.T.: The steady-state and internal redox and state (NADH/NAD) and reflects and the external and redox state and is correlated and with catabolic adaption in Escherichia coli. J. Bacteriol. 181, 2351–2357 (1999)
Lakowicz, J.R.: Principles of Fluorescence Spectroscopy. Springer, New York (2006)
Errington, J.: Regulation of endospore formation in Bacillus subtilis. Nat. Rev. Microbiol. 1, 117–126 (2003). https://doi.org/10.1038/nrmicro750
Hill, S.C., Pan, Y.-L., Williamson, C., Santarpia, J.L., Hill, H.H.: Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria: errata. Opt. Express. 22, 22817–22819 (2014)
Fischbach, T., Aleksejev, V., Duschek, F., Hausmann, A., Pargmann, C., Poryvkina, L., Sobolev, I., Babichenko, S., Handke, J.: Standoff detection of chemical and biological substances using laser induced fluorescence technique. In: 9th Future Security, Security Research Conference, Proceedings. 9th Future Security, Security Research Conference, pp. 327–334, Fraunhofer Verlag, Berlin, 16–18 Sept 2014 (2014)
Walter, A., Duschek, F., Fellner, L., Grünewald, K.M., Hausmann, A., Julich, S., Pargmann, C., Tomaso, H., Handke, J.: Standoff detection: distinction of bacteria by hyperspectral laser induced fluorescence. Int. Soc. Opt. Photon. 9824, 98240Y (2016). https://doi.org/10.1117/12.2223769
Setlow, P.: Germination of spores of bacillus species: what we know and do not know. J. Bacteriol. 196, 1297–1305 (2014)
Sarasanandarajah, S., Kunnil, J., Bronk, B.V., Reinisch, L.: Two-dimensional multiwavelength fluorescence spectra of dipicolinic acid and calcium dipicolinate. Appl. Opt. 44, 1182–1187 (2005). https://doi.org/10.1364/AO.44.001182
Pan, Y.-L.: Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence. J. Quant. Spectrosc. Radiat. Transf. 150, 12–35 (2015)
Pan, Y.-L., Hill, S.C., Santarpia, J.L., Brinkley, K., Sickler, T., Coleman, M., Williamson, C., Gurton, K., Felton, M., Pinnick, R.G., Baker, N., Eshbaugh, J., Hahn, J., Smith, E., Alvarez, B., Prugh, A., Gardner, W.: Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory. Opt. Express. 22, 8165–8189 (2014). https://doi.org/10.1364/OE.22.008165
Hill, S.C., Pinnick, R.G., Niles, S., Pan, Y.-L., Holler, S., Chang, R.K., Bottiger, J., Chen, B.T., Orr, C.-S., Feather, G.: Real-time measurement of fluorescence spectra from single airborne biological particles. Field Anal. Chem. Technol. 3, 221–239 (1999). https://doi.org/10.1002/(SICI)1520-6521(1999)3:4/5<221::AID-FACT2>3.0.CO;2-7
Katz, A., Alimova, A., Minko, G., Savage, H.E., Will, D.V., Shah, M., Rosen, R.B., Alfano, R.R.: In situ changes in native fluorescence from Bacillus subtilis during endospore formation. Proc. SPIE. 4965, 134–137 (2003)
Kraus, M., Fellner, L., Gebert, F., Grünewald, K., Pargmann, C., Walter, A., Duschek, F.: Comparison of classification models of spectral data of laser induced fluorescence. In: 1st Scientific International Conference on CBRNE (2017)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this paper
Cite this paper
Fellner, L., Gebert, F., Walter, A., Grünewald, K., Duschek, F. (2018). Variations in Fluorescence Spectra of a Bacterial Population During Different Growth Phases. In: Malizia, A., D'Arienzo, M. (eds) Enhancing CBRNE Safety & Security: Proceedings of the SICC 2017 Conference. Springer, Cham. https://doi.org/10.1007/978-3-319-91791-7_3
Download citation
DOI: https://doi.org/10.1007/978-3-319-91791-7_3
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-91790-0
Online ISBN: 978-3-319-91791-7
eBook Packages: Physics and AstronomyPhysics and Astronomy (R0)