Abstract
Transient global brain ischemia, induced by cardiac arrest and resuscitation, results in reperfusion injury leading to delayed selective neuronal cell loss and post-resuscitation mortality. This study determined the effects of post-resuscitation hypotension and hypothermia on long-term survival following cardiac arrest and resuscitation. The capillary density was also determined. Based on the mean arterial blood pressure (MABP) at 1 h of recovery, the normotension group (MABP 80–120 mmHg) and hypotension group (MABP <80 mmHg) were defined. The overall survival was determined at 4 days of recovery. Brain microvascular density was assessed using immunohistochemistry of the glucose transporter, GLUT-1. The pre-arrest MABP was similar in each group; at 1 h after resuscitation, the MABP in the normotension groups was about 80% of their pre-arrest values; the hypotension group had a significantly lower MABP compared to the normotension group. The overall survival rate was lower in the hypotension group compared to the normotension group (36%, 4/11 vs. 67%, 14/21) under the normothermic condition. Brain blood flow in the hypotension group was lower (33% decrease) compared to the normotension group at 1-h post-resuscitation. Compared to the pre-arrest baseline, the capillary density was significantly increased at 14 days of recovery (355 ± 42 vs. 469 ± 50, number/mm2) in the cortex. The capillary density in hippocampus was also increased at 4–30 days following cardiac arrest and resuscitation. Our results suggest that rats able to maintain their post-resuscitation blood pressure at normotension, had higher brain blood flow during the early recovery phase, and improved survival outcome following cardiac arrest and resuscitation. In addition, cardiac arrest and resuscitation induced angiogenesis in brain in the first month of recovery.
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References
Xu K, LaManna JC (2009) The loss of hypoxic ventilatory responses following resuscitation after cardiac arrest in rats is associated with failure of long-term survival. Brain Res 1258:59–64
Xu K, Puchowicz MA, Sun X et al (2010) Decreased brainstem function following cardiac arrest and resuscitation in aged rat. Brain Res 1328:181–189
Xu K, LaManna JC (2006) Chronic hypoxia and the cerebral circulation. J Appl Physiol 100:725–730
Chavez JC, LaManna JC (2002) Activation of hypoxia-inducible factor-1 in the rat cerebral cortex after transient global ischemia: potential role of insulin-like growth factor-1. J Neurosci 22:8922–8931
Xu K, Puchowicz MA, Lust WD et al (2006) Adenosine treatment delays postischemic hippocampal CA1 loss after cardiac arrest and resuscitation in rats. Brain Res 1071:208–217
Palkovits M, Brownstein MJ (1988) Maps and guide to microdissection of rat brain. Elsevier, New York
Benderro GF, LaManna JC (2014) HIF-1α/COX-2 expression and mouse brain capillary remodeling during prolonged moderate hypoxia and subsequent re-oxygenation. Brain Res 1569:41–47
Tsipis CP, Sun X, Xu K et al (2014) Hypoxia-induced angiogenesis and capillary density determination. Methods Mol Biol 1135:69–80
Hossmann V, Hossmann KA (1973) Return of neuronal functions after prolonged cardiac arrest. Brain Res 60(2):423–438
Brucken A, Derwall M, Bleilevens C et al (2015) Brief inhalation of nitric oxide increases resuscitation success and improves 7-day-survival after cardiac arrest in rats: a randomized controlled animal study. Crit Care 19:408
Safar P, Stezoski W, Nemoto EM (1976) Amelioration of brain damage after 12 minutes’ cardiac arrest in dogs. Arch Neurol 33(2):91–95
Ameloot K, De Deyne C, Ferdinande B et al (2017) Mean arterial pressure of 65 mm hg versus 85–100 mm Hg in comatose survivors after cardiac arrest: rationale and study design of the Neuroprotect post-cardiac arrest trial. Am Heart J 191:91–98
Acknowledgments
This study was supported by NIH grant NINDS 1 R01 NS46074.
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Xu, K., Puchowicz, M.A., LaManna, J.C. (2018). Post-resuscitation Arterial Blood Pressure on Survival and Change of Capillary Density Following Cardiac Arrest and Resuscitation in Rats. In: Thews, O., LaManna, J., Harrison, D. (eds) Oxygen Transport to Tissue XL. Advances in Experimental Medicine and Biology, vol 1072. Springer, Cham. https://doi.org/10.1007/978-3-319-91287-5_13
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DOI: https://doi.org/10.1007/978-3-319-91287-5_13
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