Abstract
Doublecortin (DCX)-immunoreactive (-ir) cells play important roles in adult cortical remodeling. We previously reported that DCX-ir cells decrease after transient global brain ischemia (GBI) in the cingulate cortex (Cg) of rats. In the present study, we examined the changes of DCX-ir cells from the acute to the chronic phase after GBI in rats. Transient GBI was induced by a four-vessel occlusion model as described previously. Thirty-six rats were divided into six groups: day 7 after sham operation (Group Sham+A), day 7 after 3 min GBI (Group GBI3+A), day 7 after 10 min GBI (Group GBI10+A), day 90 after sham operation (Group Sham+C), day 90 after 3 min GBI (Group GBI3+C), and day 90 after 10 min GBI (Group GBI10+C). The numbers of DCX-ir cells per unit area (mm2) were investigated in the anterior cingulate cortex (ACC) and retrosplenial cortex (RS). A two-way factorial analysis of variance regarding the time of GBI (sham, GBI3, GBI10) or the period after GBI (day 7, day 90) was employed in each area. Regarding the time of GBI, there were significant differences in both the ACC and the RS (p < 0.001, respectively). Regarding the period after GBI, there was no significant difference in the ACC, whereas a significant difference was found in the RS (p = 0.005). In each area and in each phase, the numbers did not change in GBI3 (one-way ANOVA followed by a Tukey test) and decreased in GBI10 (p < 0.005). The numbers in the RS from the acute phase to chronic phase did not change in the sham and GBI3, and decreased in GBI10 (independent t-test, p < 0.001). However, histochemical staining with Fluoro-Jade B suggested that neuronal cell death did not occur in both the ACC and the RS in all groups. The present findings indicate that the cortical remodeling potential in the Cg decreases in the acute phase after GBI, and continues to decrease until the chronic phase.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Francis F, Koulakoff A, Boucher D, Chafey P, Schaar B, Vinet MC, Friocourt G, McDonnell N, Reiner O, Kahn A, McConnell SK, Berwald-Netter Y, Denoulet P, Chelly J (1999) Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons. Neuron 23:247–256
Brown JP, Couillard-Despres S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG (2003) Transient expression of doublecortin during adult neurogenesis. J Comp Neurol 467:1–10
Friocourt G, Liu JS, Antypa M, Rakic S, Walsh CA, Parnavelas JG (2007) Both doublecortin and doublecortin-like kinase play a role in cortical interneuron migration. J Neurosci 27:3875–3883
Kronenberg G, Reuter K, Steiner B, Brabdt MD, Jessberger S, Yamaguchi M, Kempermann G (2003) Subpopulations of proliferating cells of the adult hippocampus respond differently to physiologic neurogenic stimuli. J Comp Neurol 467:455–463
Cai Y, Xiong K, Chu Y, Luo DW, Luo XG, Yuan XY, Struble RG, Clough RW, Spencer DD, Williamson A, Kordower JH, Patrylo PR, Yan XX (2009) Doublecortin expression in adult cat and primate cerebral cortex relates to immature neurons that develop into GABAergic subgroups. Exp Neurol 216:342–356
Kutsuna N, Suma T, Takada Y, Yamashita A, Oshima H, Sakatani K, Yamamoto T, Katayama Y (2012) Decrease in doublecortin expression without neuronal cell death in rat retrosplenial cortex after stress exposure. Neuroreport 23:211–215
Kutsuna N, Murata Y, Eriguchi T, Takada Y, Oshima H, Sakatani K, Katayama Y (2013) DCX-expressing neurons decrease in the retrosplenial cortex after global brain ischemia. Adv Exp Med Biol 765:115–121
Kutsuna N, Yamashita A, Eriguchi T, Oshima H, Suma T, Sakatani K, Yamamoto T, Yoshino A, Katayama Y (2014) Acute stress exposure preceding transient global brain ischemia exacerbates the decrease in cortical remodeling potential in the rat retrosplenial cortex. Neurosci Res 78:65–71
Nicholson BD (2004) Evaluation and treatment of cerebral pain syndromes. Neurology 62:S30–S36
Tahta K, Cirak B, Pakdemiri E, Suzer T, Tahta F (2007) Postoperative mutism after removal of an anterior falcine meningioma. J Clin Neurosci 14:793–796
Yagita Y, Kitagawa K, Otsuki T, Takasawa K, Miyata T, Okano H, Hori M, Matsumoto M (2001) Neurogenesis by progenitor cells in the ischemic adult rat hippocampus. Stroke 32:1890–1896
Paxinos G, Eatson C (2008) The rat brain in stereotaxic coordinators: compact, 6th edn. Academic, New York
Schmued LC, Hopkins KJ (2000) Fluoro-jade B: a high affinity fluorescent marker for the localization of neuronal degeneration. Brain Res 874:123–130
Zhou AM, Li WB, Li QJ, Liu HQ, Feng RF, Zhao HG (2004) A short cerebral ischemic preconditioning up-regulates adenosine receptors in the hippocampal CA1 region of rats. Neurosci Res 48:397–404
Von Bohlen H (2007) Immunohistological markers for staging neurogenesis in adult hippocampus. Cell Tissue Res 329:409–420
Kawaguchi Y, Kondo S (2002) Parvalbumin, somatostatin and cholecystokinin as chemical markers for specific GABAergic interneuron types in the rat frontal cortex. J Neurocytol 31:277–287
Kubota Y (2014) Untangling GABAergic wiring in the cortical microcircuit. Curr Opin Neurobiol 26:7–14
Xie Y, Chen S, Wu Y, Murphy TH (2014) Prolonged deficits in parvalbumin neuron stimulation-evoked network activity despite recovery of dendritic structure and excitability in mice. J Neuro-Oncol 34:14890–14900
Acknowledgments
We express our thanks to the Animal Housing Facility, Nihon University School of Medicine, the Division of Applied System Neuroscience, Department of Advanced Medical Science, Nihon University School of Medicine, and the Division of Anatomical Science, Department of Functional Morphology, Nihon University School of Medicine.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this chapter
Cite this chapter
Goto, K., Kutsuna, N., Yamashita, A., Oshima, H., Suma, T., Yoshino, A. (2018). Changes of Doublecortin-Immunoreactive Cells from the Acute Phase to Chronic Phase After Transient Global Brain Ischemia in Rat Cingulate Cortex. In: Thews, O., LaManna, J., Harrison, D. (eds) Oxygen Transport to Tissue XL. Advances in Experimental Medicine and Biology, vol 1072. Springer, Cham. https://doi.org/10.1007/978-3-319-91287-5_12
Download citation
DOI: https://doi.org/10.1007/978-3-319-91287-5_12
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-91285-1
Online ISBN: 978-3-319-91287-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)