Abstract
Fraser fir ( Abies fraseri [Pursh] Poir.) is a coniferous species native to the Southern Appalachian Mountains of the Eastern United States. Fraser fir has high economic and recreational value but is vulnerable to extinction due to introduced pests and global warming. Somatic embryogenesis may assist in the clonal production of Christmas trees and conservation of rare and valuable germplasm via cryopreservation. Improved protocols for embryogenic tissue initiation, culture capture and growth, somatic embryo development and maturation and cryogenic storage are presented based on the findings of Pullman (47:453–480, 2016).
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Acknowledgements
We thank the NC Christmas Tree Association and Institute of Paper Science and Technology at Georgia Tech (Renewable Bioproducts Institute) for providing funding, materials and supplies. We also thank Katie Olsen, Taylor Fischer, Ulrika Egertsdotter, Robert Thomas, Lilian Matallana, and Kylie Bucalo for help in protocol development.
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Pullman, G.S., Frampton, J. (2018). Fraser Fir (Abies fraseri [Pursh] Poir.). In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_3
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DOI: https://doi.org/10.1007/978-3-319-89483-6_3
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