Abstract
The holm oak is an evergreen tree species representative of Mediterranean forests. The largest populations of this species are in the Iberian Peninsula, forming the tree cover of an agro-silvo-pastoral system called “dehesa” in Spain and “montado” in Portugal. The high ecological and economic value of the species is of interest for rural development. The main current product are the acorns used for feeding Iberian black pigs, which are the basis of an important gastronomic industry. In addition, several bioactive compounds with new potential industrial applications in nutrition, pharmacology and cosmetics have been identified in acorns of oak species. Plantations with holm oak seedlings mycorrhized with Tuber melanosporum are been carried out for the production of the highly demanded black truffle. Vegetative propagation can be a main tool in the domestication of this forest species. Cloning plants from tissues of adult donor trees is desirable because selection is more reliable at mature stages. The present chapter deals with plant regeneration protocols by somatic embryogenesis from tissues of adult trees. The induction in male catkins, developing ovules and apexes and expanding leaves from in vitro cultured shoots is described. A description of methods for the cryopreservation of embryogenic lines is also provided.
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Acknowledgements
This work is being supported by research projects co-financed by the Spanish Government and the EU (AGL2013-47400-C4 and AGL2016-76143-C4).
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Corredoira, E. et al. (2018). Holm Oak Quercus ilex L.. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_14
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