Abstract
The commercial importance of coconut products has been growing very rapidly in whereas the production of nuts is decreasing because of old age and disease such as those caused by phytoplasmas. In order to maintain the growing coconut industry it is very important that most coconut cultivation surface is renewed worldwide in a short time. This cannot be achieved by seed propagation and the alternative is micropropagation through somatic embryogenesis. A protocol for such a process is described and it is based on the use of rachilla explants from immature inflorescences. The use of this type of explant allows us to clone mature palms of known performance. This protocol involves the use of different media based on the formulation of Y3 medium and additional components. These media are useful for the induction of embryogenic callus from the explants, the formation of somatic embryos on these calluses, germination of somatic embryos and their conversion into plantlets.
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References
Batugal P, Rao RV, Oliver J (2005) Coconut genetic resources. International Plant Genetic Resources Institute—Regional Office for Asia, the Pacific and Oceania (IPGRI-APO). Serdang, Selangor DE, Malaysia, pp XII–XIV
Blake J (1990) Coconut (Cocos nucifera L.): micropropagation. In: Bajaj YPS (ed) Legumes and oilseed crops I. Biotechnology in agriculture and forestry, vol 10. Springer, Berlin, pp 538–554
Blake J, Hornung R (1995) Somatic embryogenesis in coconut (Cocos nucifera L.). In: Jain S, Gupta P, Newton R (eds) Somatic embryogenesis in woody plants, vol 2. Kluwer Academic Publishers, Dordrecht, pp 327–340
Blake J, Robert M, Taylor F, Navarro C, Hornung R, Oropeza C (1994) Studies on the in vitro propagation and cloning of elite and disease resistant coconut palms. Final Report (The European Commission, Project C11.0764.M)
Chan JL, Sáenz L, Talavera C, Hornung R, Robert M, Oropeza C (1998) Regeneration of coconut (Cocos nucifera L.) from plumule explants through somatic embryogenesis. Plant Cell Rep 17:515–521
Eeuwens CJ (1976) Mineral requirements for growth and callus initiation of tissue explants excised from mature coconut (Cocos nucifera) and date (Phoenix dactylifera) palms cultured in vitro. Physiol Plant 36:23–28
Hornung R (1995) Micropropagation of Cocos nucifera (L.) from plumular tissue excised from mature zygotic embryos. Plant Rech Dev 2:38–41
Hornung R, Verdeil JL (1999) Somatic embryogenesis in coconut from immature inflorescence plumular explants. In: Oropeza C, Verdeil JL, Ashburner GR, Cardeña R, Santamaría J (eds) Current advances in coconut biotechnology. Kluwer Academic Publishers, The Netherlands, pp 297–308
Lao DA (2009) Coco-biodiesel in the Philippines. In: Coconut Philippines published by Asia Outsourcing
Oropeza C, Sáenz L, Chan L, Sandoval G, Pérez-Núñez G, Narvaez M, Rodríguez G, Borroto C (2016) Coconut micropropagation in México using plumule and floral explants. Cord 32:21–26
Perera PIP, Hocher V, Verdeil JL, Doulbeau S, Yakandawala DMD, Weerakoon LK (2007) Unfertilized ovary: a novel explant for coconut (Cocos nucifera L.) somatic embryogenesis. Plant Cell Rep 26:21–28
Perera PIP, Perera L, Hocher V, Verdeil JL, Yakandawala DMD, Weerakoon LK (2008) Use of SSR markers to determine the anther-derived homozygous lines in coconut. Plant Cell Rep 27:1697–1703
Perera PIP, Hocher V, Weerakoon LK, Yakandawala DMD, Fernando SC, Verdeil JL (2010) Early inflorescence and floral development in Cocos nucifera L. (Arecaceae: Arecoideae). S Afr J Bot 76(3):482–492
Pérez-Núñez MT, Chan JL, Sáenz L, González T, Verdeil JL, Oropeza C (2006) Improved somatic embryogenesis from Cocos nucifera (L.) plumule explants cultured in vitro. In Vitro Cell Develop Biol—Plant 42:37–43
Roolant L (2014) https://transferwise.com/blog/2014-05/why-coconut-water-is-now-a-1-billion-industry/[02/11/2015]. Why coconut water is now a one billion industry
Sandoval-Cancino G, Sáenz L, Chan JL, Oropeza C (2016) Improved formation of embryogenic callus from coconut immature inflorescence explants. In vitro Cell Develop Biol—Plant 52:367–378
Sáenz L, Oropeza C, Chan JL, Hornung R, Rillo E, Verdeil JL (1999) The use of plumular explants for the regeneration of coconut. In: Oropeza C, Verdeil JL, Ashburner GR, Cardeña R, Santamaría J (eds) Current advances in coconut biotechnology. Kluwer Academic Publishers, The Netherlands, pp 309–319
Sáenz L, Azpeitia A, Chuc-Armendariz B, Chan JL, Verdeil JL, Hocher V, Oropeza C (2006) Morphological and histological changes during somatic embryo formation from coconut plumule explants. In vitro Cell Develop Biol—Plant 42:19–25
Sáenz-Carbonell L, Montero-Cortés M, Pérez-Nuñez T, Azpeitia-Morales A, Andrade-Torres A, Córdova-Lara I, Chan-Rodríguez JL, Sandoval-Cancino G, Rivera-Solis G, Oropeza-Salín C (2016) Somatic Embryogenesis in Cocos nucifera L. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Somatic embryogenesis: fundamental aspects and applications. Springer International Publishing, Switzerland, pp 297–318
Verdeil JL, Huet C, Grosdemange F, Buffard-Morel J (1994) Plant regeneration from cultured immature inflorescences of coconut (Cocos nucifera L.): evidence for somatic embryogenesis. Plant Cell Rep 13:218–221
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Oropeza, C., Sandoval-Cancino, G., Sáenz, L., Narváez, M., Rodríguez, G., Chan, J.L. (2018). Coconut (Cocos nucifera L.) Somatic Embryogenesis Using Immature Inflorescence Explants. In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-319-79087-9_8
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