Abstract
Auxin 2,4-dichlorophenoxyacetic acid (2,4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes as explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 μM 2,4-D. Somatic embryos were observed in embryonic axes explants cultured on MS medium enriched with 1 μM 2,4-D.
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References
Akula A, Becker D, Bateson M (2000) High-yielding repetitive somatic embryogenesis and plant recovery in a selected tea clone, ‘TRI-2025’, by temporary immersion. Plant Cell Rep 19(12):1140–1145
Begum A, Ahmad I, Prodhan SH, Azad AK, Sikder MBH, Ara MR (2015) Study on in vitro propagation of tea [Camellia sinensis (L.) O. Kuntze] through different explants. J Glob Biosci 4(7):2878–2887
Benelli C, Fabbri A, Grassi S, Lambardi M, Rugini E (2001) Histology of somatic embryogenesis in mature tissue of olive (Olea europaea L.). J Hortic Sci Biotechnol 76:112–119
Diettrich B, Schneider V, Luckner M (1991) High variation in cardenolide content of plants regenerated from protoplasts of the embryogenic cell strain VII of Digitalis lanata. J Plant Physiol 139:199–204
Faisal M, Alatar A, Ahmad N, Anis M, Hegazy AK (2012) Assessment of genetic fidelity in Rauvolfia serpentina plantlets grown from synthetic (encapsulated) seeds following in vitro storage at 4 °C. Mol 17:5050–5061
Germana MA, Chiancone B (2003) Improvement of Citrus clementine Hort. ex Tan. Microspore-derived embryoid production and regeneration. Plant Cell Rep 22:181–187
Ghanati F, Ragmati Ishka M (2009) Investigation of the interaction between abscisic acid (ABA) and excess benzy-adenine (BA) on the formation of shoot in tissue culture of tea (Camellia sinensis L.). J Plant Prod 3(4):735–743
Jain SM, Ochatt SJ (2010) Protocols for in vitro propagation of ornamental plants. Humana Press, Springer Protocols
Kothari SL, Joshi A, Kachhwaha S, Ochoa-Alejo N (2010) Chilli peppers—a review on tissue culture and transgenesis. Biotech Adv 28:35–48
Litz RE, Gray DJ (1995) Somatic embryogenesis for agricultural improvement. World J Microbiol Biotechnol 11:416–425
Merkle SA (1997) Somatic embryogenesis in ornamentals. In: Geneve RL et al (eds) Biotechnol ornam plants. CAB International, Wallingford, pp 13–33
Mondal TK, Bhattacharya A, Sood A, Ahuja PS (1998) Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using thidiazuron. Plant Growth Reg 26:57–61
Mondal TK, Bhattacharya A, Ahuja PS (2001) Induction of synchronous secondary somatic embryogenesis in Camellia sinensis (L.) O. Kuntze. J Plant Physiol 158(7):945–951
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:73–497
Pedroso CM, Pais MS (1995) Factors controlling somatic embryogenesis. Plant Cell Tiss Org Cult 43:147–154
Pierik RLM (1987) In vitro propagation of higher plants. Martinus Nizhoof Publishers, Boston
Stasolla C, Yeung EC (2003) Recent advances in conifer somatic embryogenesis: improving somatic embryo quality. Plant Cell Tiss Org Cult 74:15–35
Suganthi M, Arvinth S, Raj Kumar R (2012) Impact of osmotic and abscisic acid on direct somatic embryogenesis in tea. Int J Plant Res 2(2):22–27
Wang YH, Bhalla PL (2004) Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br Plant Cell Rep 22:408–414
Yaacob JS, Yussof AIM, Taha RM, Mohajer S (2012) Somatic embryogenesis and plant regeneration from bulb, leaf and root explants of African blue lily (Agapanthus praecox ssp. Minimus). Aus J Crop Sci 6(10):1462–1470
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Kaviani, B. (2018). Tea (Camellia sinensis L.). In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-319-79087-9_20
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DOI: https://doi.org/10.1007/978-3-319-79087-9_20
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