Abstract
Syphilis is a sexually transmitted disease caused by bacteria called Treponema pallidum, and range from asymptomatic to severe. Patients with suspected syphilis should undergo a non-treponemal screening test (rapid plasma reagin) and positive results must be confirmed with a test specific to T. pallidum antigen. Here, the treponemal test, T. pallidum hemagglutination test, principle, reagents required, steps, and results interpretation in addition to examples to clarify the results interpretation process are described.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Peeling RW, Mabey DC. Syphilis. Nat Rev Microbiol. 2004;2(6):448–9.
Sena AC, White BL, Sparling PF. Novel Treponema pallidum serologic tests: a paradigm shift in syphilis screening for the 21st century. Clin Infect Dis. 2010;51(6):700–8.
Baker-Zander SA, Roddy RE, Handsfield HH, Lukehart SA. IgG and IgM antibody reactivity to antigens of Treponema pallidum after treatment of syphilis. Sex Transm Dis. 1986;13(4):214–20.
Baughn RE, Jorizzo JL, Adams CB, Musher DM. Ig class and IgG subclass responses to Treponema pallidum in patients with syphilis. J Clin Immunol. 1988;8(2):128–39.
Merlin S, Andre J, Alacoque B, Paris-Hamelin A. Importance of specific IgM antibodies in 116 patients with various stages of syphilis. Genitourin Med. 1985;61(2):82–7.
BIOLABO. Haemagglutination assay for qualitative and semi-quantitative determination of antibodies to Treponema pallidum in human serum or plasma. BIOLABO. 2014. Accessed 2 September 2017. Available from: http://www.biolabo.fr/biolabo/pdfs/noticesE/syphilisE/AT-45-00-100-TPHA.
Human Diagnostics Worldwide. Syphilis TPHA liquid. Human-de. 2014. Accessed 2 September 2017. Available from: http://www.human-de.com/data/gb/vr/lx-tpha.pdf.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this chapter
Cite this chapter
Alhabbab, R.Y. (2018). Treponema pallidum Hemagglutination (TPHA) Test. In: Basic Serological Testing. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-319-77694-1_5
Download citation
DOI: https://doi.org/10.1007/978-3-319-77694-1_5
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-77693-4
Online ISBN: 978-3-319-77694-1
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)