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Treponema pallidum Hemagglutination (TPHA) Test

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Abstract

Syphilis is a sexually transmitted disease caused by bacteria called Treponema pallidum, and range from asymptomatic to severe. Patients with suspected syphilis should undergo a non-treponemal screening test (rapid plasma reagin) and positive results must be confirmed with a test specific to T. pallidum antigen. Here, the treponemal test, T. pallidum hemagglutination test, principle, reagents required, steps, and results interpretation in addition to examples to clarify the results interpretation process are described.

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References

  1. Peeling RW, Mabey DC. Syphilis. Nat Rev Microbiol. 2004;2(6):448–9.

    Article  CAS  PubMed  Google Scholar 

  2. Sena AC, White BL, Sparling PF. Novel Treponema pallidum serologic tests: a paradigm shift in syphilis screening for the 21st century. Clin Infect Dis. 2010;51(6):700–8.

    Article  PubMed  Google Scholar 

  3. Baker-Zander SA, Roddy RE, Handsfield HH, Lukehart SA. IgG and IgM antibody reactivity to antigens of Treponema pallidum after treatment of syphilis. Sex Transm Dis. 1986;13(4):214–20.

    Article  CAS  PubMed  Google Scholar 

  4. Baughn RE, Jorizzo JL, Adams CB, Musher DM. Ig class and IgG subclass responses to Treponema pallidum in patients with syphilis. J Clin Immunol. 1988;8(2):128–39.

    Article  CAS  PubMed  Google Scholar 

  5. Merlin S, Andre J, Alacoque B, Paris-Hamelin A. Importance of specific IgM antibodies in 116 patients with various stages of syphilis. Genitourin Med. 1985;61(2):82–7.

    PubMed  PubMed Central  CAS  Google Scholar 

  6. BIOLABO. Haemagglutination assay for qualitative and semi-quantitative determination of antibodies to Treponema pallidum in human serum or plasma. BIOLABO. 2014. Accessed 2 September 2017. Available from: http://www.biolabo.fr/biolabo/pdfs/noticesE/syphilisE/AT-45-00-100-TPHA.

  7. Human Diagnostics Worldwide. Syphilis TPHA liquid. Human-de. 2014. Accessed 2 September 2017. Available from: http://www.human-de.com/data/gb/vr/lx-tpha.pdf.

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Alhabbab, R.Y. (2018). Treponema pallidum Hemagglutination (TPHA) Test. In: Basic Serological Testing. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-319-77694-1_5

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