Skip to main content

Enzymology and Nanoparticles

  • Chapter
  • First Online:
Nanobotany
  • 450 Accesses

Abstract

Enzymes are the natural biocatalysts which are being used now in a number of industries such as food industry, laundry and medicine etc. Now their use has also been established in the production of biosensors, polymerase chain reactions and biofuel cells etc. Activity of enzymes has been increased by using nanotechnology in collaboration by using nanoparticles as immobilizing agents. Immobilized enzymes have been proved to be a better system in terms of purity of product, feasibility of reaction, control of system etc. Enzymes are used again and again by this process. Enzyme nanoparticles have also been produced which increase the efficiency of the system. There are different methods to produce ENPs which are described in detail. Nanoparticles can also be used as co-factors for the commercially exploited important enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Beck JS, Vertuli JC, Roth WJ, Leonowicz ME, Kresge CT, Schmitt KD, Chu CTW, Olson DH, Sheppard EW, Mccullen SB, Higgins JB, Schelker JL (1992) A new family of mesoporous molecular sieves prepared with liquid crystal templates. J Am Chem Soc 114:10834–10843

    Article  CAS  Google Scholar 

  • Blanchette C, Lacayo CI, Fischer NO, Hawang M, Thelen MP (2012) Enhanced cellulose degradation using cellulose nanospheres. PLoS One 7:e42116

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Celestino KRS, Cunha RB, Felix CR (2006) Characterization of a β-glucanase produced by rhizopus microsporus var. Microsporus, and its potential for application in the brewing industry. BMC Biochem 7(1):1

    Article  CAS  Google Scholar 

  • Chauhan N, Kumar A, Pundir CS (2014) Construction of an uricase nanoparticles modified Au electrode for amperometric determination of uric acid. App Biochem Biotechnol 174:1683–1694

    Article  CAS  Google Scholar 

  • Chawla S, Rawal R, Sonia R, Pundir CS (2013) Preparation of cholesterol oxidase nanoparticles and their application in amperometric determination of cholesterol. J Nanopart Res 15:1934–1943

    Article  Google Scholar 

  • Choct M (2006) Enzymes for the feed industry: past, present and future. Worlds Poult Sci J 62(01):5–16

    Article  Google Scholar 

  • D’Souza SF (1999) Immobilized enzymes in bioprocess. Curr Sci 77:69–79

    Google Scholar 

  • Datta S, Christena LR, Rajaram YRS (2013) Enzyme immobilization: an overview on techniques and support materials. Biotech 3:1–9

    Google Scholar 

  • Dyal A, Loos K, Noto M, Chang SW, Spagnoli C, Shafi K, Ulman A, Cowman M, Gross RA (2003) Activity of Candida regusa lipase immobilized on gamma Fe2O3 magnetic nanoparticles. J Am Chem Soc 125:1684–1685

    Article  CAS  PubMed  Google Scholar 

  • Fetzner S (2002) Oxygenases without requirement for cofactors or metal ions. Appl Microbiol Biotech 60(3):243–257

    Article  CAS  Google Scholar 

  • Haard N, Simpson B (1994) Proteases from aquatic organisms and their uses in the seafood industry. Fisheries processing. Springer, pp 132–154

    Chapter  Google Scholar 

  • Haq I, Ali S, Javed M, Hameed U, Saleem A, Adnan F, Qadeer M (2010) Production of alpha amylase from a randomly induced mutant strain of Bacillus amyloliquefaciens and its application as a desizer in textile industry. Pak J Bot 42(1):473–484

    CAS  Google Scholar 

  • Houde A, Kademi A, Leblanc D (2004) Lipases and their industrial applications. Appl Biochem Biotech 118(1–3):155–170

    Article  CAS  Google Scholar 

  • Hvolbæk B, Janssens TVW, Bjerne SC, Hanne F, Claus HC, Jens KN (2007) Catalytic activity of Au nanoparticles. NanoToday 2(4):14–19

    Article  Google Scholar 

  • Jia HF, Zhu GY, Vugrinovich B, Kataphinan W, Reneker DH, Wang P (2002) Enzyme carrying polymeric nanofibers prepared via electrospinning for use as unique biocatalysts. Bitech Prog 18:1027–1032

    Article  CAS  Google Scholar 

  • Kim J, Grate JW (2003) Single enzyme nanoparticles armored by a nanometer scale organic/inorganic network. Nano Lett 3:1219–1222

    Article  CAS  Google Scholar 

  • Kuhad RC, Gupta R, Singh A (2011) Microbial cellulases and their industrial applications. Enzyme Res Article ID 280696, 10 pages

    Google Scholar 

  • Kundu N, Yadav S, Pundir CS (2012) Preparation and characterization of glucose oxidase nanoparticles and their application in DO metric determination of serum glucose. J Nanosci Nanotechnol 13:1710–1716

    Article  CAS  Google Scholar 

  • Liu G, Lin Y, Ostatna V, Wang J (2005) Enzyme nanoparticles based electronic biosensor. Chem Commun (27):3481–3483

    Google Scholar 

  • Maurer KH (2004) Detergent proteases. Curr Opin Biotech 15(4):330–334

    Article  CAS  PubMed  Google Scholar 

  • Naseeb S, Sohail M, Ahmad A, Khan SA (2015) Production of xylanases and cellulases by Aspergillus fumigatus ms16 using crude lignocellulosic substrates. Pak J Bot 47:779–784

    CAS  Google Scholar 

  • Nilius N, Ganduglia-Pirovano MV, Brazdova V, Kulawik M, Sauer J, Freund HJ (2008) Counting electrons transferred through a thin alumina film into Au chains. Phys Rev Lett 100

    Google Scholar 

  • Ota Y, Yamada K (1966) Lipase from candida paralipolytica: Part ii. Alkaline earth metal ions as the cofactor in the shaken system containing no emulsifier. Agr Biol Chem 30(10):1030–1038

    CAS  Google Scholar 

  • Pundir C (2015) Enzyme nanoparticles. Preparation, characterisation, properties and applications. eBook ISBN: 9780323389297, Elsevier

    Google Scholar 

  • Schauermann S, Nilius N, Shaikhutdinov S, and Freund HJ (2013) Nanoparticles for Heterogeneous Catalysis: New Mechanistic Insights. Acc Chem Res 46 (8): 1673–1681

    Article  CAS  PubMed  Google Scholar 

  • Scheffel U, Rhodes BA, Natrajan TK, Wagner HN (1972) Albumin microspheres for study of reticuloendothelial system. J Nucl Med 13:498–503

    PubMed  CAS  Google Scholar 

  • Schwarz G, Mendel RR, Ribbe MW (2009) Molybdenum cofactors, enzymes and pathways. Nature 460(7257):839–847

    Article  CAS  PubMed  Google Scholar 

  • Shao X, Prada S, Giordano L, Pacchioni G, Nilius N, Freund HJ (2011) Tailoring the shape of metal ad-particles by doping the oxide support. Angew Chem Int Ed 50:11525–11527

    Article  CAS  Google Scholar 

  • Sharma S, Shrivastav A, Gupta N, Srivastava S (2011) Amperometric biosensor: increased sensitivity using enzyme nanoparticles. In: 2010 International conference on nanotechnology and biosensors. IPCBEE 2:2426

    Google Scholar 

  • Souza PMD (2010) Application of microbial α-amylase in industry-a review. Braz J Microbiol 41(4):850–861

    Article  PubMed  PubMed Central  Google Scholar 

  • Sterrer M, Risse T, Heyde M, Rust HP, Freund HJ (2007) Crossover from three dimensional to two-dimensional geometries of Au nanostructures on thin MgO (001) films: a confirmation of theoretical predictions. Phys Rev Lett 98:206103–206101

    Article  CAS  PubMed  Google Scholar 

  • Wang J, Sheppard GS, Lou P, Kawai M, Park C, Egan DA, Schneider A, Bouska J, Lesniewski R, Henkin J (2003) Physiologically relevant metal cofactor for methionine aminopeptidase-2 is manganese. Biochemistry 42(17):5035–5042

    Article  CAS  PubMed  Google Scholar 

  • Wu C, Jim TF, Gan Z, Zhao Y, Wang S (2000) A heterogeneous catalytic kinetics for enzymatic biodegradation of poly(ϵ-caprolactone) nanoparticles in aqueous solution. Polymer 41(10):3593–3597

    Article  CAS  Google Scholar 

  • Yan M, Jun G, Pingkai O (2006) Encapsulation of single enzyme in nanogel with enhanced biocatalytic activity and stability. J Am Chem Soc 128:11008–11009

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Javad, S., Jabeen, K. (2018). Enzymology and Nanoparticles. In: Javad, S., Butt, A. (eds) Nanobotany. Springer, Cham. https://doi.org/10.1007/978-3-319-77119-9_8

Download citation

Publish with us

Policies and ethics