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All Optical Label-Free Detection, Imaging and Tracking of Single Proteins

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Investigation of Nanoscopic Dynamics and Potentials by Interferometric Scattering Microscopy

Part of the book series: Springer Theses ((Springer Theses))

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Abstract

This chapter details a proof of principle study with the molecular motor myosin 5a heavy meromyosin, which demonstrates that interferometric scattering microscopy can be used as a truly label-free all-optical bio-sensing platform operating at the single-molecule level.

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References

  1. Ortega Arroyo, J., et al.: Label-free, all-optical detection, imaging, and tracking of a single protein. Nano. Lett. 14, 2065–2070 (2014)

    Google Scholar 

  2. Nie, S., Emory, S.R.: Probing single molecules and single nanoparticles by surface-enhanced Raman scattering. Science 275, 1102–1106 (1997)

    Article  CAS  Google Scholar 

  3. Thacker, V.V., et al.: DNA origami based assembly of gold nanoparticle dimers for surface-enhanced Raman scattering. Nat. Commun. 5, 3448 (2014)

    Article  Google Scholar 

  4. Zijlstra, P., Paulo, P.M.R., Orrit, M.: Optical detection of single non-absorbing molecules using the surface plasmon resonance of a gold nanorod. Nat. Nanotechnol. 7, 379–382 (2012)

    Article  CAS  Google Scholar 

  5. Ament, I., Prasad, J., Henkel, A., Schmachtel, S., Sönnichsen, C.: Single unlabeled protein detection on individual plasmonic nanoparticles. Nano. Lett. 12, 1092–1095 (2012)

    Article  CAS  Google Scholar 

  6. Beuwer, M.A., Prins, M.W.J., Zijlstra, P.: Stochastic protein interactions monitored by hundreds of single-molecule plasmonic biosensors. Nano. Lett. 15, 3507–3511 (2015)

    Article  CAS  Google Scholar 

  7. Dantham, V.R., et al.: Label-free detection of single protein using a nanoplasmonic-photonic hybrid microcavitys. Nano. Lett. 13, 3347–3351 (2013)

    Article  CAS  Google Scholar 

  8. Baaske, M.D., Foreman, M.R., Vollmer, F.: Single-molecule nucleic acid interactions monitored on a label-free microcavity biosensor platform. Nat. Nanotechnol. 9, 933–939 (2014)

    Article  CAS  Google Scholar 

  9. Kukura, P., Celebrano, M., Renn, A., Sandoghdar, V.: Single-molecule sensitivity in optical absorption at room temperature. J. Phys. Chem. Lett. 1, 3323–3327 (2010)

    Article  CAS  Google Scholar 

  10. Chong, S., Min, W., Xie, X.S.: Ground-state depletion microscopy: detection sensitivity of single-molecule optical absorption at room temperature. J. Phys. Chem. Lett. 1, 3316–3322 (2010)

    Article  CAS  Google Scholar 

  11. Gaiduk, A., Yorulmaz, M., Ruijgrok, P.V., Orrit, M.: Room-temperature detection of a single molecule’s absorption by photothermal contrast. Science 330, 353–356 (2010)

    Article  CAS  Google Scholar 

  12. Sellers, J.R., Veigel, C.: Walking with myosin V. Curr. Opin. Cell. Biol. 18, 68–73 (2006)

    Article  CAS  Google Scholar 

  13. Spudich, J.A., Watt, S.: The regulation of rabbit skeletal muscle contraction I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J. Biol. Chem. 246, 4866–4871 (1971)

    CAS  Google Scholar 

  14. Wang, F., et al.: Effect of ADP and ionic strength on the kinetic and motile properties of recombinant mouse myosin V. J. Biol. Chem. 275, 4329–4335 (2000)

    Article  CAS  Google Scholar 

  15. Kukura, P., et al.: High-speed nanoscopic tracking of the position and orientation of a single virus. Nat. Methods 6, 923–927 (2009)

    Article  CAS  Google Scholar 

  16. Kubitscheck, U., Kückmann, O., Kues, T., Peters, R.: Imaging and tracking of single GFP molecules in solution. Biophys. J. 78, 2170–2179 (2000)

    Article  CAS  Google Scholar 

  17. Yildiz, A., et al.: Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science 300, 2061–2065 (2003)

    Article  CAS  Google Scholar 

  18. Rief, M., et al.: Myosin-V stepping kinetics: a molecular model for processivity. Proc. Natl. Acad. Sci. USA 97, 9482–9486 (2000)

    Article  CAS  Google Scholar 

  19. Snyder, G.E., Sakamoto, T., Hammer III, J.A., Sellers, J.R., Selvin, P.R.: Nanometer localization of single green fluorescent proteins: evidence that myosin V walks hand-over-hand via telemark configuration. Biophys. J. 87, 1776–1783 (2004)

    Article  CAS  Google Scholar 

  20. Baker, J.E., et al.: Myosin V processivity: multiple kinetic pathways for head-to-head coordination. Proc. Natl. Acad. Sci. USA 101, 5542–5546 (2004)

    Article  CAS  Google Scholar 

  21. Perkins, T.T.: Ångström-precision optical traps and applications*. Annu. Rev Biophys 43, 279–302 (2014)

    Article  CAS  Google Scholar 

  22. Piliarik, M., Sandoghdar, V.: Direct optical sensing of single unlabelled proteins and super-resolution imaging of their binding sites. Nat. Commun. 5, 4495 (2014)

    Article  CAS  Google Scholar 

  23. Andrecka, J., Spillane, K.M., Ortega Arroyo, J., Kukura, P.: Direct observation and control of supported lipid bilayer formation with interferometric scattering microscopy. ACS Nano. 7, 10662–10670 (2013)

    Google Scholar 

  24. Cohen, S.I.A., et al.: A molecular chaperone breaks the catalytic cycle that generates toxic A\(\beta \) oligomers. Nat. Struct. Mol. Biol. 22, 207–213 (2015)

    Article  CAS  Google Scholar 

  25. Dumont, E.L.P., Do, C., Hess, H.F.: Molecular wear of microtubules propelled by surface-adhered kinesins. Nat. Nanotechnol. 10, 166–169 (2015)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. ICFO—The Institute of Photonic Sciences, Barcelona, Spain

    Jaime Ortega Arroyo

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  1. Jaime Ortega Arroyo
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Correspondence to Jaime Ortega Arroyo .

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Ortega Arroyo, J. (2018). All Optical Label-Free Detection, Imaging and Tracking of Single Proteins. In: Investigation of Nanoscopic Dynamics and Potentials by Interferometric Scattering Microscopy. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-77095-6_6

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