Skip to main content

Head and Neck: Thyroid

  • Chapter
  • First Online:
Atlas of Fine Needle Aspiration Cytology

Abstract

Thyroid nodules are a frequent finding in the general population, with palpable nodules found in 4–7% of adults and subclinical nodules identified in up to 70% of adults, although the vast majority (90–95%) of these nodules are benign. Since its introduction in the early 1950s, fine-needle aspiration (FNA) of the thyroid has proven to be an integral and reliable method of evaluating patients presenting with a thyroid mass. The addition of thyroid FNA to clinical evaluation of a thyroid nodule has dramatically increased triage efficiency: not only determining which nodules are at greatest risk of malignancy and should undergo surgery but also indicating which nodules are benign, thus sparing the patient unnecessary surgery. As such, thyroid FNA acts as both a diagnostic test and a screening test.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 219.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 279.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Clarke DP, Faquin WC. Thyroid cytopathology. 2nd ed. New York: Springer; 2010.

    Book  Google Scholar 

  2. Soderstrom N. Puncture of goiters for aspiration biopsy. Acta Med Scand. 1952;144:237–44.

    Article  CAS  Google Scholar 

  3. Miller JM, Hamburger JI, Kini S. Diagnosis of thyroid nodules. Use of fine-needle aspiration and needle biopsy. JAMA. 1979;241:481–4.

    Article  CAS  Google Scholar 

  4. Haugen BR, Alexander EK, Bible KC, et al. 2015 American Thyroid Association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: the American Thyroid Association guidelines task force on thyroid nodules and differentiated thyroid cancer. Thyroid. 2016;26:1–133.

    Article  Google Scholar 

  5. Jung CK, Lee A, Jung ES, et al. Split sample comparison of a liquid-based method and conventional smears in thyroid fine needle aspiration. Acta Cytol. 2008;52:313–9.

    Article  Google Scholar 

  6. Fischer AH, Clayton AC, Bentz JS, et al. Performance differences between conventional smears and liquid-based preparations of thyroid fine-needle aspiration samples: analysis of 47,076 responses in the College of American Pathologists Interlaboratory Comparison Program in Non-Gynecologic Cytology. Arch Pathol Lab Med. 2013;137:26–31.

    Article  Google Scholar 

  7. Sanchez N, Selvaggi SM. Utility of cell blocks in the diagnosis of thyroid aspirates. Diagn Cytopathol. 2006;34:89–92.

    Article  Google Scholar 

  8. Na DG, Kim JH, Sung JY, et al. Core-needle biopsy is more useful than repeat fine-needle aspiration in thyroid nodules read as nondiagnostic or atypia of undetermined significance by the Bethesda system for reporting thyroid cytopathology. Thyroid. 2012;22:468–75.

    Article  Google Scholar 

  9. Wang HH. Reporting thyroid fine-needle aspiration: literature review and a proposal. Diagn Cytopathol. 2006;34:67–76.

    Article  Google Scholar 

  10. Cibas ES, Ali SZ. The Bethesda system for reporting thyroid cytopathology. Am J Clin Pathol. 2009;132:658–65.

    Article  Google Scholar 

  11. Ali SZ, Cibas ES (Eds.). The Bethesda system for reporting thyroid cytopathology: definitions, criteria and explanatory notes. 2nd edition. Cham, Switzerland: Springer International Publishing AG; 2018.

    Google Scholar 

  12. Cross PA, Chandra A, Giles T, et al. Guidance on the reporting of thyroid cytology specimens, version number 2. 2016. https://www.rcpath.org/resourceLibrary/g089-guidancereportingthyroidcytology-jan16.html. Accessed 20 Jul 2017.

  13. Nguyen GK, Lee MW, Ginsberg J, et al. Fine-needle aspiration of the thyroid: an overview. Cytojournal. 2005;2:12.

    Article  Google Scholar 

  14. Faquin WC, Cibas ES, Renshaw AA. “Atypical” cells in fine-needle aspiration biopsy specimens of benign thyroid cysts. Cancer. 2005;105:71–9.

    Article  Google Scholar 

  15. Tseleni-Balafouta S, Gakiopoulou H, Kavantzas N, et al. Parathyroid proliferations: a source of diagnostic pitfalls in FNA of thyroid. Cancer. 2007;111:130–6.

    Article  Google Scholar 

  16. Harvey AM, Truong LD, Mody DR. Diagnostic pitfalls of Hashimoto’s/lymphocytic thyroiditis on fine-needle aspirations and strategies to avoid overdiagnosis. Acta Cytol. 2012;56:352–60.

    Article  Google Scholar 

  17. Anderson SR, Mandel S, LiVolsi VA, et al. Can cytomorphology differentiate between benign nodules and tumors arising in Graves’ disease? Diagn Cytopathol. 2004;31:64–7.

    Article  Google Scholar 

  18. García Solano J, Giménez Bascuñana A, Sola Pérez J, et al. Fine-needle aspiration of subacute granulomatous thyroiditis (De Quervain’s thyroiditis): a clinico-cytologic review of 36 cases. Diagn Cytopathol. 1997;16:214–20.

    Article  Google Scholar 

  19. Wan SK, Chan JK, Tang SK. Paucicellular variant of anaplastic thyroid carcinoma. A mimic of Reidel’s thyroiditis. Am J Clin Pathol. 1996;105:388–93.

    Article  CAS  Google Scholar 

  20. Somma J, Schlecht NF, Fink D, et al. Thyroid fine needle aspiration cytology: follicular lesions and the gray zone. Acta Cytol. 2010;54:123–31.

    Article  Google Scholar 

  21. Renshaw AA. Hürthle cell carcinoma is a better gold standard than Hürthle cell neoplasm for fine-needle aspiration of the thyroid: defining more consistent and specific cytologic criteria. Cancer. 2002;96:261–6.

    Article  Google Scholar 

  22. Elliott DD, Pitman MB, Bloom L, Faquin WC. Fine-needle aspiration biopsy of Hurthle cell lesions of the thyroid gland: a cytomorphologic study of 139 cases with statistical analysis. Cancer. 2006;108:102–9.

    Article  Google Scholar 

  23. Wu HH, Clouse J, Ren R. Fine-needle aspiration cytology of Hürthle cell carcinoma of the thyroid. Diagn Cytopathol. 2008;36:149–54.

    Article  Google Scholar 

  24. Kaur A, Jayaram G. Thyroid tumors: cytomorphology of papillary carcinoma. Diagn Cytopathol. 1991;7:462–8.

    Article  CAS  Google Scholar 

  25. Kini SR, Miller JM, Hamburger JI, Smith MJ. Cytopathology of papillary carcinoma of the thyroid by fine needle aspiration. Acta Cytol. 1980;24:511–21.

    CAS  PubMed  Google Scholar 

  26. Akhtar M, Ali MA, Huq M, Bakry M. Fine-needle aspiration biopsy of papillary thyroid carcinoma: cytologic, histologic, and ultrastructural correlations. Diagn Cytopathol. 1991;7:373–9.

    Article  CAS  Google Scholar 

  27. Das DK, Sharma PN. Diagnosis of papillary thyroid carcinoma in fine needle aspiration smears: factors that affect decision making. Acta Cytol. 2009;53:497–506.

    Article  Google Scholar 

  28. Mijovic T, Gologan O, Rochon L, et al. Fine-needle aspiration biopsy of the thyroid: review of cytopathologic features predictive of malignancy. J Otolaryngol Head Neck Surg. 2009;38:348–54.

    PubMed  Google Scholar 

  29. Punthakee X, Palme CE, Franklin JH, et al. Fine-needle aspiration biopsy findings suspicious for papillary thyroid carcinoma: a review of cytopathological criteria. Laryngoscope. 2005;115:433–6.

    Article  Google Scholar 

  30. Miller TR, Bottles K, Holly EA, et al. A step-wise logistic regression analysis of papillary carcinoma of the thyroid. Acta Cytol. 1986;30:285–93.

    CAS  PubMed  Google Scholar 

  31. Zhang Y, Fraser JL, Wang HH. Morphologic predictors of papillary carcinoma on fine-needle aspiration of thyroid with ThinPrep preparations. Diagn Cytopathol. 2001;24:378–83.

    Article  CAS  Google Scholar 

  32. Nikiforov YE, Seethala RR, Tallini G, et al. Nomenclature revision for encapsulated follicular variant of papillary thyroid carcinoma: a paradigm shift to reduce overtreatment of indolent tumors. JAMA Oncol. 2016;2:1023–9.

    Article  Google Scholar 

  33. Strickland KC, Howitt BE, Marqusee E, et al. The impact of noninvasive follicular variant of papillary thyroid carcinoma on rates of malignancy for fine-needle aspiration diagnostic categories. Thyroid. 2015;25:987–92.

    Article  Google Scholar 

  34. Faquin WC, Wong LQ, Afrogheh AH, et al. Impact of reclassifying noninvasive follicular variant of papillary thyroid carcinoma on the risk of malignancy in The Bethesda System for Reporting Thyroid Cytopathology. Cancer Cytopathol. 2016;124:181–7.

    Article  Google Scholar 

  35. Strickland KC, Vivero M, Jo VY, et al. Preoperative cytologic diagnosis of noninvasive follicular thyroid neoplasm with papillary-like nuclear features: a prospective analysis. Thyroid. 2016;26:1466–71.

    Article  Google Scholar 

  36. Krane JF, Alexander EK, Cibas ES, Barletta JA. Coming to terms with NIFTP: a provisional approach for cytologists. Cancer Cytopathol. 2016;124:767–72.

    Article  Google Scholar 

  37. Howitt BE, Chang S, Eszlinger M, et al. Fine-needle aspiration diagnoses of noninvasive follicular variant of papillary thyroid carcinoma. Am J Clin Pathol. 2015;144:850–7.

    Article  CAS  Google Scholar 

  38. Brandler TC, Zhou F, Liu CZ, et al. Can noninvasive follicular thyroid neoplasm with papillary-like nuclear features be distinguished from classic papillary thyroid carcinoma and follicular adenomas by fine-needle aspiration? Cancer Cytopathol. 2017;125:378–88.

    Article  CAS  Google Scholar 

  39. Maletta F, Massa F, Torregrossa L, et al. Cytological features of “noninvasive follicular thyroid neoplasm with papillary-like nuclear features” and their correlation with tumor histology. Hum Pathol. 2016;54:134–42.

    Article  CAS  Google Scholar 

  40. Aron M, Mallik A, Verma K. Fine needle aspiration cytology of follicular variant of papillary carcinoma of the thyroid: morphologic pointers to its diagnosis. Acta Cytol. 2006;50:663–8.

    Article  Google Scholar 

  41. Zhao L, Dias-Santagata D, Sadow PM, Faquin WC. Cytological, molecular, and clinical features of noninvasive follicular thyroid neoplasm with papillary-like nuclear features versus invasive forms of follicular variant of papillary thyroid carcinoma. Cancer. 2017;125:323–31.

    CAS  Google Scholar 

  42. Chung D, Ghossein RA, Lin O. Macrofollicular variant of papillary carcinoma: a potential thyroid FNA pitfall. Diagn Cytopathol. 2007;35:560–4.

    Article  Google Scholar 

  43. Mesonero CE, Jugle JE, Wilbur DC, Nayar R. Fine-needle aspiration of the macrofollicular and microfollicular subtypes of the follicular variant of papillary carcinoma of the thyroid. Cancer. 1998;84:235–44.

    Article  CAS  Google Scholar 

  44. Castro-Gómez L, Córdova-Ramírez S, Duarte-Torres R, et al. Cytologic criteria of cystic papillary carcinoma of the thyroid. Acta Cytol. 2003;47:590–4.

    Article  Google Scholar 

  45. Muller N, Cooperberg PL, Suen KC, Thorson SC. Needle aspiration biopsy in cystic papillary carcinoma of the thyroid. AJR Am J Roentgenol. 1985;144:251–3.

    Article  CAS  Google Scholar 

  46. Harshan M, Crapanzano JP, Aslan DL, et al. Papillary thyroid carcinoma with atypical histiocytoid cells on fine-needle aspiration. Diagn Cytopathol. 2009;37:244–50.

    Article  Google Scholar 

  47. Renshaw AA. Histiocytoid cells in fine needle aspirates of papillary carcinoma of the thyroid: frequency and significance of an under-recognized cytologic pattern. Cancer Cytopathol. 2002;96:240–3.

    Article  Google Scholar 

  48. Urano M, Kiriyama Y, Takakuwa Y, Kuroda M. Tall cell variant of papillary thyroid carcinoma: its characteristic features demonstrated by fine-needle aspiration cytology and immunohistochemical study. Diagn Cytopathol. 2009;37:732–7.

    Article  Google Scholar 

  49. Das DK, Mallik MK, Sharma P, et al. Papillary thyroid carcinoma and its variants in fine needle aspiration smears. A cytomorphologic study with special reference to tall cell variant. Acta Cytol. 2004;48:325–36.

    Article  Google Scholar 

  50. Solomon A, Gupta PK, LiVolsi VA, Baloch ZW. Distinguishing tall cell variant of papillary thyroid carcinoma from usual variant of papillary thyroid carcinoma in cytologic specimens. Diagn Cytopathol. 2002;27:143–8.

    Article  Google Scholar 

  51. Bocklage T, DiTomasso JP, Ramzy I, Ostrowski ML. Tall cell variant of papillary thyroid carcinoma: cytologic features and differential diagnostic considerations. Diagn Cytopathol. 1997;17:25–9.

    Article  CAS  Google Scholar 

  52. Leung AK, Chow SM, Law SC. Clinical features and outcome of the tall cell variant of papillary thyroid carcinoma. Laryngoscope. 2008;118:32–8.

    Article  Google Scholar 

  53. Montone KT, Baloch ZW, LiVolsi VA. The thyroid Hürthle (oncocytic) cell and its associated pathologic conditions: a surgical pathology and cytopathology review. Arch Pathol Lab Med. 2008;132:1241–50.

    PubMed  Google Scholar 

  54. Lee J, Hasteh F. Oncocytic variant of papillary thyroid carcinoma associated with Hashimoto’s thyroiditis: a case report and review of the literature. Diagn Cytopathol. 2009;37:600–6.

    Article  Google Scholar 

  55. Mai KT, Thomas J, Yazdi HM, et al. Pathologic study and clinical significance of Hürthle cell papillary thyroid carcinoma. Appl Immunohistochem Mol Morphol. 2004;12:329–37.

    Article  Google Scholar 

  56. Paker I, Kokenek TD, Yılmazer D, et al. Oncocytic variant of papillary thyroid carcinoma with lymphocytic stroma (Warthin-like variant): report of a case with fine needle aspiration cytology and review of the literature. Cytopathology. 2012;23:408–10.

    Article  CAS  Google Scholar 

  57. Baloch ZW, Livolsi VA. Warthin-like papillary carcinoma of the thyroid. Arch Pathol Lab Med. 2000;124:1192–5.

    CAS  PubMed  Google Scholar 

  58. Evans HL. Columnar-cell carcinoma of the thyroid. A report of two cases of an aggressive variant of thyroid carcinoma. Am J Clin Pathol. 1986;85:77–80.

    Article  CAS  Google Scholar 

  59. Jayaram G. Cytology of columnar-cell variant of papillary thyroid carcinoma. Diagn Cytopathol. 2000;22:227–9.

    Article  CAS  Google Scholar 

  60. Hirokawa M, Maekawa M, Kuma S, Miyauchi A. Cribriform-morular variant of papillary thyroid carcinoma––cytological and immunocytochemical findings of 18 cases. Diagn Cytopathol. 2010;38:890–6.

    Article  Google Scholar 

  61. Koo JS, Jung W, Hong SW. Cytologic characteristics and β-catenin immunocytochemistry on smear slide of cribriform-morular variant of papillary thyroid carcinoma. Acta Cytol. 2011;55:13–8.

    Article  CAS  Google Scholar 

  62. Lee JY, Shin JH, Han BK, et al. Diffuse sclerosing variant of papillary carcinoma of the thyroid: imaging and cytologic findings. Thyroid. 2007;17:567–73.

    Article  CAS  Google Scholar 

  63. Kumarasinghe MP. Cytomorphologic features of diffuse sclerosing variant of papillary carcinoma of the thyroid. A report of two cases in children. Acta Cytol. 1998;42:983–6.

    Article  CAS  Google Scholar 

  64. Casey MB, Sebo TJ, Carney JA. Hyalinizing trabecular adenoma of the thyroid gland: cytologic features in 29 cases. Am J Surg Pathol. 2004;28:859–67.

    Article  Google Scholar 

  65. Bishop JA, Ali SZ. Hyalinizing trabecular adenoma of the thyroid gland. Diagn Cytopathol. 2011;39:306–10.

    Article  Google Scholar 

  66. Casey MB, Sebo TJ, Carney JA. Hyalinizing trabecular adenoma of the thyroid gland identification through MIB-1 staining of fine-needle aspiration biopsy smears. Am J Clin Pathol. 2004;122:506–10.

    Article  Google Scholar 

  67. Skalova A, Vanecek T, Sima R, et al. Mammary analogue secretory carcinoma of salivary glands, containing the ETV6-NTRK3 fusion gene: a hitherto undescribed salivary gland tumor entity. Am J Surg Pathol. 2010;34(5):599–608.

    PubMed  Google Scholar 

  68. Reynolds S, Shaheen M, Olson G, et al. A case of primary mammary analog secretory carcinoma (MASC) of the thyroid masquerading as papillary thyroid carcinoma: potentially more than a one off. Head Neck Pathol. 2016;10:405–13.

    Article  CAS  Google Scholar 

  69. Dogan S, Wang L, Ptashkin RN, et al. Mammary analog secretory carcinoma of the thyroid gland: a primary thyroid adenocarcinoma harboring ETV6-NTRK3 fusion. Mod Pathol. 2016;29:985–95.

    Article  CAS  Google Scholar 

  70. Dettloff J, Seethala RR, Stevens TM, et al. Mammary analog secretory carcinoma (MASC) involving the thyroid gland: a report of the first 3 cases. Head Neck Pathol Head Neck Pathol. 2017;11:124–30.

    Article  Google Scholar 

  71. Wu EY, Lebastchi J, Marqusee E, et al. A case of primary secretory carcinoma of the thyroid gland with high-grade features. Histopathology. 2017;71:665–9.

    Article  Google Scholar 

  72. Rupp A, Blockage T. Mammary analog secretory carcinoma of thyroid: a case report. Diagn Cytopathol. 2017;45:45–50.

    Article  Google Scholar 

  73. Farhat NA, Onenerk MA, Krane JF, et al. Primary benign and malignant thyroid neoplasms with signet ring cells. Am J Clin Pathol. 2017;148:251–8.

    Article  Google Scholar 

  74. Pietribiasi F, Sapino A, Papotti M, Bussolati G. Cytologic features of poorly differentiated “insular” carcinoma of the thyroid, as revealed by fine-needle aspiration biopsy. Am J Clin Pathol. 1990;94:687–92.

    Article  CAS  Google Scholar 

  75. Sironi M, Collini P, Cantaboni A. Fine needle aspiration cytology of insular thyroid carcinoma. A report of four cases. Acta Cytol. 1992;36:435–9.

    CAS  PubMed  Google Scholar 

  76. Bongiovanni M, Boom L, Krane JF, et al. Cytomorphologic features of poorly differentiated thyroid carcinoma: a multi-institutional study of 40 cases. Cancer Cytopathol. 2009;117:185–94.

    Article  Google Scholar 

  77. Barwad A, Dey P, Nahar Saikia U, et al. Fine needle aspiration cytology of insular carcinoma of thyroid. Diagn Cytopathol. 2012;40(Suppl 1):E43–7.

    Article  Google Scholar 

  78. Green I, Ali SZ, Allen EA, Zakowski MF. A spectrum of cytomorphologic variations in medullary thyroid carcinoma. Fine-needle aspiration findings in 19 cases. Cancer. 1997;81:40–4.

    Article  CAS  Google Scholar 

  79. Papaparaskeva K, Nagel H, Droese M. Cytologic diagnosis of medullary carcinoma of the thyroid gland. Diagn Cytopathol. 2000;22:351–8.

    Article  CAS  Google Scholar 

  80. Zeppa P, Vetrani A, Marino M, et al. Fine needle aspiration cytology of medullary thyroid carcinoma: a review of 18 cases. Cytopathology. 1990;1:35–44.

    Article  CAS  Google Scholar 

  81. Bose S, Kapila K, Verma K. Medullary carcinoma of the thyroid: a cytological, immunocytochemical, and ultrastructural study. Diagn Cytopathol. 1992;8:28–32.

    Article  CAS  Google Scholar 

  82. Rivera M, Sang C, Gerhard R, et al. Anaplastic thyroid carcinoma: morphologic findings and PAX-8 expression in cytology specimens. Acta Cytol. 2010;54:668–72.

    Article  Google Scholar 

  83. Brooke PK, Hameed M, Zakowski MF. Fine-needle aspiration of anaplastic thyroid carcinoma with varied cytologic and histologic patterns: a case report. Diagn Cytopathol. 1994;11:60–3.

    Article  CAS  Google Scholar 

  84. Guarda LA, Peterson CE, Hall W, Baskin HJ. Anaplastic thyroid carcinoma: cytomorphology and clinical implications of fine-needle aspiration. Diagn Cytopathol. 1991;7:63–7.

    Article  CAS  Google Scholar 

  85. Bishop JA, Sharma R, Westra WH. PAX8 immunostaining of anaplastic thyroid carcinoma: a reliable means of discerning thyroid origin for undifferentiated tumors of the head and neck. Hum Pathol. 2011;42:1873–7.

    Article  CAS  Google Scholar 

  86. Ha CS, Shadle KM, Medeiros LJ, et al. Localized non-Hodgkin lymphoma involving the thyroid gland. Cancer. 2001;91:629–35.

    Article  CAS  Google Scholar 

  87. Derringer GA, Thompson LD, Frommelt RA, et al. Malignant lymphoma of the thyroid gland: a clinicopathologic study of 108 cases. Am J Surg Pathol. 2000;24:623–39.

    Article  CAS  Google Scholar 

  88. Smith SA, Gharib H, Goellner JR. Fine-needle aspiration. Usefulness for diagnosis and management of metastatic carcinoma to the thyroid. Arch Intern Med. 1987;147:311–2.

    Article  CAS  Google Scholar 

  89. Lee MW, Batoroev YK, Odashiro AN, Nguyen GK. Solitary metastatic cancer to the thyroid: a report of five cases with fine-needle aspiration cytology. Cytojournal. 2007;4:5.

    Article  Google Scholar 

  90. Haugen BR, Nawaz S, Cohn A, et al. Secondary malignancy of the thyroid gland: a case report and review of the literature. Thyroid. 1994;4:297–300.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jeffrey F. Krane .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

VanderLaan, P.A., Krane, J.F. (2019). Head and Neck: Thyroid. In: Domanski, H. (eds) Atlas of Fine Needle Aspiration Cytology. Springer, Cham. https://doi.org/10.1007/978-3-319-76980-6_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-76980-6_5

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-76979-0

  • Online ISBN: 978-3-319-76980-6

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics