Skip to main content

Introduction

  • Chapter
  • First Online:
Atlas of Fine Needle Aspiration Cytology

Abstract

Fine-needle aspiration cytology (FNAC) has been widely used as a tool to obtain specimens for the morphological diagnosis of numerous lesions in a variety of locations for more than 80 years. In many clinical situations, FNAC can render a definitive diagnosis either from aspiration smears alone using well-defined cytological criteria or from smears combined with clinical data, radiological findings, and the results of ancillary studies. Today, this technique is more powerful than ever in making rapid preliminary diagnoses in neoplastic and nonneoplastic conditions, guiding further work-up of the patient or even allowing the initiation of definitive treatment. The use of ever more sophisticated ancillary methods on aspiration specimens, such as molecular/genetic analysis and immunocytochemistry, allows a diagnosis of tumors that can be used for predicting prognosis and tailoring individualized “targeted” oncological therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 219.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 279.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Webb AJ. Through a glass darkly. (The development of needle aspiration biopsy). Bristol Med Chir J. 1974;89(332):59–68.

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Frable WJ. Fine-needle aspiration biopsy: a review. Hum Pathol. 1983;14(1):9–28.

    Article  CAS  PubMed  Google Scholar 

  3. Martin HE, Ellis EB. Biopsy by needle puncture and aspiration. Ann Surg. 1930;92(2):169–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Stewart FW. The diagnosis of tumors by aspiration. Am J Pathol. 1933;9(Suppl):801–12. 3.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Soderstrom N. Puncture of goiters for aspiration biopsy. Acta Med Scand. 1952;144(3):237–44.

    Article  CAS  PubMed  Google Scholar 

  6. Linsk JA. Aspiration cytology in Sweden: the Karolinska group. Diagn Cytopathol. 1985;1(4):332–5.

    Article  CAS  PubMed  Google Scholar 

  7. Stimmer L, Dehay S, Nemati F, Massonnet G, Richon S, Decaudin D, et al. Human breast cancer and lymph node metastases express Gb3 and can be targeted by STxB-vectorized chemotherapeutic compounds. BMC Cancer. 2014;14:916.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  8. Treece AL, Montgomery ND, Patel NM, Civalier CJ, Dodd LG, Gulley ML, et al. FNA smears as a potential source of DNA for targeted next-generation sequencing of lung adenocarcinomas. Cancer Cytopathol. 2016;124(6):406–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Li W, Zhang Z, Guo L, Qiu T, Ling Y, Cao J, et al. Assessment of cytology based molecular analysis to guide targeted therapy in advanced non-small-cell lung cancer. Oncotarget. 2016;7(7):8332–40.

    PubMed  Google Scholar 

  10. Karnes HE, Duncavage EJ, Bernadt CT. Targeted next-generation sequencing using fine-needle aspirates from adenocarcinomas of the lung. Cancer Cytopathol. 2014;122(2):104–13.

    Article  CAS  PubMed  Google Scholar 

  11. Young G, Wang K, He J, Otto G, Hawryluk M, Zwirco Z, et al. Clinical next-generation sequencing successfully applied to fine-needle aspirations of pulmonary and pancreatic neoplasms. Cancer Cytopathol. 2013;121(12):688–94.

    Article  CAS  PubMed  Google Scholar 

  12. Acs B, Szekely N, Szasz AM, Lotz G, Szekely T, Istok R, et al. Reliability of immunocytochemistry and fluorescence in situ hybridization on fine-needle aspiration cytology samples of breast cancers: a comparative study. Diagn Cytopathol. 2016;44(6):466–71.

    Article  PubMed  Google Scholar 

  13. Lundberg M, Munsterhjelm B, Makitie A, Leivo I. Immunohistochemical staining of histological fragments derived from salivary gland tumour fine-needle biopsy aspirates. Acta Cytol. 2017;61(1):17–20.

    Article  CAS  PubMed  Google Scholar 

  14. Travis WD, Wold LE. Immunoperoxidase staining of fine needle aspiration specimens previously stained by the Papanicolaou technique. Acta Cytol. 1987;31(4):517–20.

    CAS  PubMed  Google Scholar 

  15. Bhatia P, Dey P, Uppal R, Shifa R, Srinivasan R, Nijhawan R. Cell blocks from scraping of cytology smear: comparison with conventional cell block. Acta Cytol. 2008;52(3):329–33.

    Article  PubMed  Google Scholar 

  16. Domanski HA. Fine-needle aspiration cytology of soft tissue lesions: diagnostic challenges. Diagn Cytopathol. 2007;35(12):768–73.

    Article  PubMed  Google Scholar 

  17. Weynand B, Borbath I, Galant C, Piessevaux H, Deprez PH. Optimizing specimen collection and laboratory procedures reduces the non-diagnostic rate for endoscopic ultrasound-guided fine-needle aspiration of solid lesions of the pancreas. Cytopathol Off J Br Soc Clin Cytol. 2013;24(3):177–84.

    Article  CAS  Google Scholar 

  18. Robitschek J, Straub M, Wirtz E, Klem C, Sniezek J. Diagnostic efficacy of surgeon-performed ultrasound-guided fine needle aspiration: a randomized controlled trial. Otolaryngol Head Neck Surg Off J Am Acad Otolaryngol Head Neck Surg. 2010;142(3):306–9.

    Article  Google Scholar 

  19. De Fiori E, Rampinelli C, Turco F, Bonello L, Bellomi M. Role of operator experience in ultrasound-guided fine-needle aspiration biopsy of the thyroid. Radiol Med. 2010;115(4):612–8.

    Article  PubMed  Google Scholar 

  20. Rafael OC, Klein M, Serbonich MM, Vadalia B, Das K, Gimenez CE. Young investigator challenge: building an ultrasound-guided FNA clinic-our 5-year experience: from project to practice. Cancer. 2017;125(3):161–8.

    Google Scholar 

  21. Klapman JB, Logrono R, Dye CE, Waxman I. Clinical impact of on-site cytopathology interpretation on endoscopic ultrasound-guided fine needle aspiration. Am J Gastroenterol. 2003;98(6):1289–94.

    Article  PubMed  Google Scholar 

  22. Mazza E, Maddau C, Ricciardi A, Falchini M, Matucci M, Ciarpallini T. On-site evaluation of percutaneous CT-guided fine needle aspiration of pulmonary lesions. A study of 321 cases. Radiol Med. 2005;110(3):141–8.

    PubMed  Google Scholar 

  23. Campisi P, Accinelli G, De Angelis C, Pacchioni D, Bussolati G. On-site evaluation and triage for endoscopic ultrasound-guided fine needle aspiration cytology. The Turin experience. Minerva Med. 2007;98(4):395–400.

    CAS  PubMed  Google Scholar 

  24. Zhu W, Michael CW. How important is on-site adequacy assessment for thyroid FNA? An evaluation of 883 cases. Diagn Cytopathol. 2007;35(3):183–6.

    Article  PubMed  Google Scholar 

  25. Nguyen YP, Maple JT, Zhang Q, Ylagan LR, Zhai J, Kohlmeier C, et al. Reliability of gross visual assessment of specimen adequacy during EUS-guided FNA of pancreatic masses. Gastrointest Endosc. 2009;69(7):1264–70.

    Article  PubMed  Google Scholar 

  26. Dhillon I, Pitman MB, Demay RM, Archuletta P, Shidham VB. Compensation crisis related to the onsite adequacy evaluation during FNA procedures-urgent proactive input from cytopathology community is critical to establish appropriate reimbursement for CPT code 88172 (or its new counterpart if introduced in the future). CytoJournal. 2010;7:23.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Ganguly A, Giles TE, Smith PA, White FE, Nixon PP. The benefits of on-site cytology with ultrasound-guided fine needle aspiration in a one-stop neck lump clinic. Ann R Coll Surg Engl. 2010;92(8):660–4.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Fassina A, Corradin M, Zardo D, Cappellesso R, Corbetti F, Fassan M. Role and accuracy of rapid on-site evaluation of CT-guided fine needle aspiration cytology of lung nodules. Cytopathol Off J Br Soc Clin Cytol. 2011;22(5):306–12.

    Article  CAS  Google Scholar 

  29. Iglesias-Garcia J, Dominguez-Munoz JE, Abdulkader I, Larino-Noia J, Eugenyeva E, Lozano-Leon A, et al. Influence of on-site cytopathology evaluation on the diagnostic accuracy of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) of solid pancreatic masses. Am J Gastroenterol. 2011;106(9):1705–10.

    Article  PubMed  Google Scholar 

  30. Raymond SLT, Yugawa D, Chang KHF, Ena B, Tauchi-Nishi PS. Metastatic neoplasms to the pancreas diagnosed by fine-needle aspiration/biopsy cytology: a 15-year retrospective analysis. Diagn Cytopathol. 2017;45(9):771–83.

    Article  PubMed  Google Scholar 

  31. Yu GH, Maisel J, Frank R, Pukenas BA, Sebro R, Weber K. Diagnostic utility of fine-needle aspiration cytology of lesions involving bone. Diagn Cytopathol. 2017;45(7):608–13.

    Article  PubMed  Google Scholar 

  32. Wyse J, Rubino M, Iglesias Garcia J, Sahai AV. Onsite evaluation of endoscopic ultrasound fine needle aspiration: the endosonographer, the cytotechnologist and the cytopathologist. Rev Esp Enferm Dig. 2017;109(4):279–83.

    PubMed  Google Scholar 

  33. Silverman JF, Finley JL, O'Brien KF, Dabbs DJ, Park HK, Larkin EW, et al. Diagnostic accuracy and role of immediate interpretation of fine needle aspiration biopsy specimens from various sites. Acta Cytol. 1989;33(6):791–6.

    CAS  PubMed  Google Scholar 

  34. Burlingame OO, Kesse KO, Silverman SG, Cibas ES. On-site adequacy evaluations performed by cytotechnologists: correlation with final interpretations of 5241 image-guided fine-needle aspiration biopsies. Cancer Cytopathol. 2012;120(3):177–84.

    Article  PubMed  Google Scholar 

  35. Stelow EB. Who should perform rapid or on-site assessment of thyroid fine-needle aspirations? Am J Clin Pathol. 2012;138(1):8–9.

    Article  PubMed  Google Scholar 

  36. Wangsiricharoen S, Lekawanvijit S, Rangdaeng S. Agreement between rapid on-site evaluation and the final cytological diagnosis of salivary gland specimens. Cytopathol Off J Br Soc Clin Cytol. 2017;28(4):321–8.

    Article  CAS  Google Scholar 

  37. Tamura T, Yamashita Y, Ueda K, Kawaji Y, Itonaga M, Murata SI, et al. Rapid on-site evaluation by endosonographers during endoscopic ultrasonography-guided fine-needle aspiration for diagnosis of gastrointestinal stromal tumors. Clin Endosc. 2017;50(4):372–8.

    Article  PubMed  PubMed Central  Google Scholar 

  38. Ravaioli S, Bravaccini S, Tumedei MM, Pironi F, Candoli P, Puccetti M. Easily detectable cytomorphological features to evaluate during ROSE for rapid lung cancer diagnosis: from cytology to histology. Oncotarget. 2017;8(7):11199–205.

    Article  PubMed  Google Scholar 

  39. Mfokazi A, Wright CA, Louw M, Von Groote-Bidlingmaier F, Schubert PT, Koegelenberg CF, et al. Direct comparison of liquid-based and smear-based cytology with and without rapid on-site evaluation for fine needle aspirates of thoracic tumors. Diagn Cytopathol. 2016;44(5):363–8.

    Article  CAS  PubMed  Google Scholar 

  40. Kong F, Zhu J, Kong X, Sun T, Deng X, Du Y, et al. Rapid on-site evaluation does not improve endoscopic ultrasound-guided fine needle aspiration adequacy in pancreatic masses: a meta-analysis and systematic review. PLoS One. 2016;11(9):e0163056.

    Article  PubMed  PubMed Central  Google Scholar 

  41. Kim B, Chhieng DC, Crowe DR, Jhala D, Jhala N, Winokur T, et al. Dynamic telecytopathology of on site rapid cytology diagnoses for pancreatic carcinoma. CytoJournal. 2006;3:27.

    Article  PubMed  PubMed Central  Google Scholar 

  42. Alsharif M, Carlo-Demovich J, Massey C, Madory JE, Lewin D, Medina AM, et al. Telecytopathology for immediate evaluation of fine-needle aspiration specimens. Cancer Cytopathol. 2010;118(3):119–26.

    Article  PubMed  Google Scholar 

  43. Khurana KK, Rong R, Wang D, Roy A. Dynamic telecytopathology for on-site preliminary diagnosis of endoscopic ultrasound-guided fine needle aspiration of pancreatic masses. J Telemed Telecare. 2012;18(5):253–9.

    Article  PubMed  Google Scholar 

  44. Khurana KK, Kovalovsky A, Masrani D. Feasibility of telecytopathology for rapid preliminary diagnosis of ultrasound-guided fine needle aspiration of axillary lymph nodes in a remote breast care center. J Pathol Inform. 2012;3:36.

    Article  PubMed  PubMed Central  Google Scholar 

  45. Yang YJ, Damron TA. Comparison of needle core biopsy and fine-needle aspiration for diagnostic accuracy in musculoskeletal lesions. Arch Pathol Lab Med. 2004;128(7):759–64.

    PubMed  Google Scholar 

  46. Joudeh AA, Shareef SQ, Al-Abbadi MA. Fine-needle aspiration followed by core-needle biopsy in the same setting: modifying our approach. Acta Cytol. 2016;60(1):1–13.

    Article  PubMed  Google Scholar 

  47. Domanski HA, Akerman M, Carlen B, Engellau J, Gustafson P, Jonsson K, et al. Core-needle biopsy performed by the cytopathologist: a technique to complement fine-needle aspiration of soft tissue and bone lesions. Cancer. 2005;105(4):229–39.

    Article  PubMed  Google Scholar 

  48. Ahn SH, Park SY, Choi SI. Comparison of consecutive results from fine needle aspiration and core needle biopsy in thyroid nodules. Endocr Pathol. 2017;28(4):332–8.

    Article  PubMed  Google Scholar 

  49. Saftoiu A, Vilmann P, Guldhammer Skov B, Georgescu CV. Endoscopic ultrasound (EUS)-guided Trucut biopsy adds significant information to EUS-guided fine-needle aspiration in selected patients: a prospective study. Scand J Gastroenterol. 2007;42(1):117–25.

    Article  PubMed  Google Scholar 

  50. Stewart CJ, Coldewey J, Stewart IS. Comparison of fine needle aspiration cytology and needle core biopsy in the diagnosis of radiologically detected abdominal lesions. J Clin Pathol. 2002;55(2):93–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Koscick RL, Petersilge CA, Makley JT, Abdul-Karim FW. CT-guided fine needle aspiration and needle core biopsy of skeletal lesions. Complementary diagnostic techniques. Acta Cytol. 1998;42(3):697–702.

    Article  CAS  PubMed  Google Scholar 

  52. Layfield LJ, Schmidt RL, Sangle N, Crim JR. Diagnostic accuracy and clinical utility of biopsy in musculoskeletal lesions: a comparison of fine-needle aspiration, core, and open biopsy techniques. Diagn Cytopathol. 2014;42(6):476–86.

    Article  PubMed  Google Scholar 

  53. Barwari K, Kummerlin IP, ten Kate FJ, Algaba F, Trias I, Wijkstra H, et al. What is the added value of combined core biopsy and fine needle aspiration in the diagnostic process of renal tumours? World J Urol. 2013;31(4):823–7.

    Article  CAS  PubMed  Google Scholar 

  54. Haseler LJ, Sibbitt RR, Sibbitt WL Jr, Michael AA, Gasparovic CM, Bankhurst AD. Syringe and needle size, syringe type, vacuum generation, and needle control in aspiration procedures. Cardiovasc Intervent Radiol. 2011;34(3):590–600.

    Article  PubMed  Google Scholar 

  55. Maurya AK, Mehta A, Mani NS, Nijhawan VS, Batra R. Comparison of aspiration vs non-aspiration techniques in fine-needle cytology of thyroid lesions. J Cytol Indian Acad Cytol. 2010;27(2):51–4.

    Article  Google Scholar 

  56. Braun H, Walch C, Beham A, Moinfar F. Fine needle capillary cytology versus fine needle aspiration cytology – a comparison of quality between puncture techniques in the ENT area. Laryngorhinootologie. 1997;76(6):358–63.

    Article  CAS  PubMed  Google Scholar 

  57. Zajdela A, Zillhardt P, Voillemot N. Cytological diagnosis by fine needle sampling without aspiration. Cancer. 1987;59(6):1201–5.

    Article  CAS  PubMed  Google Scholar 

  58. Lieske B, Ravichandran D, Wright D. Role of fine-needle aspiration cytology and core biopsy in the preoperative diagnosis of screen-detected breast carcinoma. Br J Cancer. 2006;95(1):62–6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. Field AS, Schmitt F, Vielh PIAC. Standardized reporting of breast fine-needle aspiration biopsy cytology. Acta Cytol. 2017;61(1):3–6.

    Article  PubMed  Google Scholar 

  60. Guidelines of the Papanicolaou Society of Cytopathology for fine-needle aspiration procedure and reporting. The Papanicolaou Society of Cytopathology Task Force on Standards of Practice. Diagn Cytopathol. 1997;17(4):239–47.

    Google Scholar 

  61. Choi YI, Jakhongir M, Choi SJ, Kim L, Park IS, Han JY, et al. High-quality cell block preparation from scraping of conventional cytology slide: a technical report on a modified cytoscrape cell block technique. Malays J Pathol. 2016;38(3):295–304.

    CAS  PubMed  Google Scholar 

  62. Lindsey KG, Houser PM, Shotsberger-Gray W, Chajewski OS, Yang J. Young investigator challenge: a novel, simple method for cell block preparation, implementation, and use over 2 years. Cancer. 2016;124(12):885–92.

    CAS  Google Scholar 

  63. Kossakowski CA, Morresi-Hauf A, Schnabel PA, Eberhardt R, Herth FJ, Warth A. Preparation of cell blocks for lung cancer diagnosis and prediction: protocol and experience of a high-volume center. Respir Int Rev Thorac Dis. 2014;87(5):432–8.

    CAS  Google Scholar 

  64. Koss LG. Diagnostic cytology and its histopathologic bases. 3d ed. Philadelphia: Lippincott; 1979.

    Google Scholar 

  65. Ren S, Solomides C, Draganova-Tacheva R, Bibbo M. Overview of nongynecological samples prepared with liquid-based cytology medium. Acta Cytol. 2014;58(6):522–32.

    Article  PubMed  Google Scholar 

  66. Kim SY, Kim EK, Moon HJ, Yoon JH, Kwon HJ, Song MK, et al. Combined use of conventional smear and liquid-based preparation versus conventional smear for thyroid fine-needle aspiration. Endocrine. 2016;53(1):157–65.

    Article  CAS  PubMed  Google Scholar 

  67. Qiu T, Zhu H, Cai M, Han Q, Shi J, Wang K. Liquid-based cytology preparation can improve cytological assessment of endobronchial ultrasound-guided transbronchial needle aspiration. Acta Cytol. 2015;59(2):139–43.

    Article  CAS  PubMed  Google Scholar 

  68. Guiter GE, Gatscha RM, Zakowski MF. ThinPrep vs. conventional smears in fine-needle aspirations of sarcomas: a morphological and immunocytochemical study. Diagn Cytopathol. 1999;21(5):351–4.

    Article  CAS  PubMed  Google Scholar 

  69. Arul P. Utility of manual liquid-based cytology and conventional smears in the evaluation of various fine-needle aspiration samples. J Cytol Indian Acad Cytol. 2016;33(4):177–81.

    Article  CAS  Google Scholar 

  70. Park GS, Lee SH, Jung SL, Jung CK. Liquid-based cytology in the fine needle aspiration of parathyroid lesions: a comparison study with the conventional smear, ThinPrep, and SurePath. Int J Clin Exp Pathol. 2015;8(10):12160–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  71. Xiong S, Liu N, Han Q, Jiang Y, Su X. Diagnostic value of endobronchial ultrasound-guided transbronchial needle aspiration combined with Surepath liquid-based cytology test for lung lesions and mediastinal lymphadenopathy. Zhonghua bing li xue za zhi Chin Journal Pathol. 2015;44(9):633–8.

    Google Scholar 

  72. Nagarajan N, Schneider EB, Ali SZ, Zeiger MA, Olson MT. How do liquid-based preparations of thyroid fine-needle aspiration compare with conventional smears? An analysis of 5475 specimens. Thyroid Off J Am Thyroid Assoc. 2015;25(3):308–13.

    Article  Google Scholar 

  73. Gerhard R, Schmitt FC. Liquid-based cytology in fine-needle aspiration of breast lesions: a review. Acta Cytol. 2014;58(6):533–42.

    CAS  PubMed  Google Scholar 

  74. Vigliar E, Malapelle U, Bellevicine C, de Luca C, Troncone G. Outsourcing cytological samples to a referral laboratory for EGFR testing in non-small cell lung cancer: does theory meet practice? Cytopathol Off J Br Soc Clin Cytol. 2015;26(5):312–7.

    Article  CAS  Google Scholar 

  75. Minca EC, Lanigan CP, Reynolds JP, Wang Z, Ma PC, Cicenia J, et al. ALK status testing in non-small-cell lung carcinoma by FISH on ThinPrep slides with cytology material. J Thorac Oncol. 2014;9(4):464–8.

    Article  CAS  PubMed  Google Scholar 

  76. Krane JF, Cibas ES, Alexander EK, Paschke R, Eszlinger M. Molecular analysis of residual ThinPrep material from thyroid FNAs increases diagnostic sensitivity. Cancer Cytopathol. 2015;123(6):356–61.

    Article  CAS  PubMed  Google Scholar 

  77. Konofaos P, Kontzoglou K, Parakeva P, Kittas C, Margari N, Giaxnaki E, et al. The role of ThinPrep cytology in the investigation of ki-67 index, p53 and HER-2 detection in fine-needle aspirates of breast tumors. J BUON. 2013;18(2):352–8.

    CAS  PubMed  Google Scholar 

  78. Scarpa Carniello JV, Pareja F, Santos-Zabala ML, Edelweiss M. Diagnostic dilemmas and pitfalls in ThinPrep(R) cytology of breast fine needle aspiration biopsy:: report of six cases with histological correlates. Diagn Cytopathol. 2017;45(7):655–61.

    Article  PubMed  PubMed Central  Google Scholar 

  79. da Cunha Santos G, Saieg MA. Preanalytic specimen triage: smears, cell blocks, cytospin preparations, transport media, and cytobanking. Cancer. 2017;125(S6):455–64.

    Google Scholar 

  80. Walther C, Domanski HA, von Steyern FV, Mandahl N, Mertens F. Chromosome banding analysis of cells from fine-needle aspiration biopsy samples from soft tissue and bone tumors: is it clinically meaningful? Cancer Genet. 2011;204(4):203–6.

    Article  CAS  PubMed  Google Scholar 

  81. Gleeson FC, Kerr SE, Kipp BR, Voss JS, Minot DM, Tu ZJ, et al. Targeted next generation sequencing of endoscopic ultrasound acquired cytology from ampullary and pancreatic adenocarcinoma has the potential to aid patient stratification for optimal therapy selection. Oncotarget. 2016;7(34):54526–36.

    Article  PubMed  PubMed Central  Google Scholar 

  82. Sho S, Court CM, Kim S, Braxton DR, Hou S, Muthusamy VR, et al. Digital PCR improves mutation analysis in pancreas fine needle aspiration biopsy specimens. PLoS One. 2017;12(1):e0170897.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  83. Nikiforov YE. Role of molecular markers in thyroid nodule management: then and now. Endocr Pract Off J Am Coll Endocrinol Am Assoc Clin Endocrinologist. 2017;23(8):979–88.

    Google Scholar 

  84. Mosele N, Smith A, Galli M, Pagni F, Magni F. MALDI-MSI analysis of cytological smears: the study of thyroid cancer. Methods Mol Biol. 2017;1618:37–47.

    Article  CAS  PubMed  Google Scholar 

  85. Lithwick-Yanai G, Dromi N, Shtabsky A, Morgenstern S, Strenov Y, Feinmesser M, et al. Multicentre validation of a microRNA-based assay for diagnosing indeterminate thyroid nodules utilising fine needle aspirate smears. J Clin Pathol. 2017;70(6):500–7.

    Article  CAS  PubMed  Google Scholar 

  86. Ko HM, Saieg MA, da Cunha Santos G, Kamel-Reid S, Boerner SL, Geddie WR. Use of cytological samples of metastatic melanoma for ancillary studies. Cytopathol Off J Br Soc Clin Cytol. 2017;28(3):221–7.

    Article  CAS  Google Scholar 

  87. Fabbri C, Gibiino G, Fornelli A, Cennamo V, Grifoni D, Visani M, et al. Team work and cytopathology molecular diagnosis of solid pancreatic lesions. Dig Endosc Off J Japan Gastroenterol Endosc Soc. 2017;87. 2017;29(6):657–66.

    Article  PubMed  Google Scholar 

  88. Lee C, Guichet PL, Abtin F. Percutaneous lung biopsy in the molecular profiling era: a survey of current practices. J Thorac Imaging. 2017;32(1):63–7.

    Article  PubMed  Google Scholar 

  89. Eszlinger M, Lau L, Ghaznavi S, Symonds C, Chandarana SP, Khalil M, et al. Molecular profiling of thyroid nodule fine-needle aspiration cytology. Nat Rev Endocrinol. 2017;13(7):415–24.

    Article  CAS  PubMed  Google Scholar 

  90. Zheng G, Lin MT, Lokhandwala PM, Beierl K, Netto GJ, Gocke CD, et al. Clinical mutational profiling of bone metastases of lung and colon carcinoma and malignant melanoma using next-generation sequencing. Cancer. 2016;124(10):744–53.

    CAS  Google Scholar 

  91. Wei S, Lieberman D, Morrissette JJ, Baloch ZW, Roth DB, McGrath C. Using “residual” FNA rinse and body fluid specimens for next-generation sequencing: an institutional experience. Cancer Cytopathol. 2016;124(5):324–9.

    Article  CAS  PubMed  Google Scholar 

  92. Tian HY, Xu D, Liu JP, Mao WM, Chen LY, Yang C, et al. Contribution of ultrasound-guided fine-needle aspiration cell blocks of metastatic supraclavicular lymph nodes to the diagnosis of lung cancer. J Cancer Res Ther. 2015;11:C234–8.

    CAS  PubMed  Google Scholar 

  93. Akhtar M, Iqbal MA, Mourad W, Ali MA. Fine-needle aspiration biopsy diagnosis of small round cell tumors of childhood: a comprehensive approach. Diagn Cytopathol. 1999;21(2):81–91.

    Article  CAS  PubMed  Google Scholar 

  94. Ravinsky E, Quinonez GE, Paraskevas M, Diocee MS, Kutryk EM. Processing fine needle aspiration biopsies for electron microscopic examination. Experience implementing a procedure. Acta Cytol. 1993;37(5):661–6.

    CAS  PubMed  Google Scholar 

  95. Akhtar M, Bakry M, al-Jeaid AS, McClintock JA. Electron microscopy of fine-needle aspiration biopsy specimens: a brief review. Diagn Cytopathol. 1992;8(3):278–82.

    Article  CAS  PubMed  Google Scholar 

  96. Sapi Z, Bodo M, Megyesi J, Rahoty P. Fine needle aspiration cytology of biphasic synovial sarcoma of soft tissue. Report of a case with ultrastructural, immunohistologic and cytophotometric studies. Acta Cytol. 1990;34(1):69–73.

    CAS  PubMed  Google Scholar 

  97. Boyd JD, Smith GD, Hong H, Mageau R, Juskevicius R. Fine-needle aspiration is superior to needle core biopsy as a sample acquisition method for flow cytometric analysis in suspected hematologic neoplasms. Cytometry B Clin Cytom. 2015;88(1):64–8.

    Article  PubMed  Google Scholar 

  98. Shetuni B, Lakey M, Kulesza P. Optimal specimen processing of fine needle aspirates of non-Hodgkin lymphoma. Diagn Cytopathol. 2012;40(11):984–6.

    Article  PubMed  Google Scholar 

  99. Brahimi M, Arabi A, Soltan BE, Osmani S, Benradouane H, Bey M, et al. How we assess adequacy of fine-needle aspiration materials intended for flow cytometric analysis. Hematol Oncol Stem Cell Ther. 2011;4(1):37–40.

    Article  CAS  PubMed  Google Scholar 

  100. Pugh JL, Jhala NC, Eloubeidi MA, Chhieng DC, Eltoum IA, Crowe DR, et al. Diagnosis of deep-seated lymphoma and leukemia by endoscopic ultrasound-guided fine-needle aspiration biopsy. Am J Clin Pathol. 2006;125(5):703–9.

    Article  PubMed  Google Scholar 

  101. Sigstad E, Dong HP, Davidson B, Berner A, Tierens A, Risberg B. The role of flow cytometric immunophenotyping in improving the diagnostic accuracy in referred fine-needle aspiration specimens. Diagn Cytopathol. 2004;31(3):159–63.

    Article  PubMed  Google Scholar 

  102. Juco J, Holden JT, Mann KP, Kelley LG, Li S. Immunophenotypic analysis of anaplastic large cell lymphoma by flow cytometry. Am J Clin Pathol. 2003;119(2):205–12.

    Article  PubMed  Google Scholar 

  103. Gong JZ, Williams DC Jr, Liu K, Jones C. Fine-needle aspiration in non-Hodgkin lymphoma: evaluation of cell size by cytomorphology and flow cytometry. Am J Clin Pathol. 2002;117(6):880–8.

    Article  PubMed  Google Scholar 

  104. Barrena S, Almeida J, Del Carmen Garcia-Macias M, Lopez A, Rasillo A, Sayagues JM, et al. Flow cytometry immunophenotyping of fine-needle aspiration specimens: utility in the diagnosis and classification of non-Hodgkin lymphomas. Histopathology. 2011;58(6):906–18.

    Article  PubMed  Google Scholar 

  105. Mancuso G, Bovio E, Rena O, Rrapaj E, Mercalli F, Veggiani C, et al. Prognostic impact of a 3-MicroRNA signature in cytological samples of small cell lung cancer. Cancer Cytopathol. 2016;124(9):621–9.

    Article  CAS  PubMed  Google Scholar 

  106. Rossi ED, Bizzarro T, Longatto-Filho A, Gerhard R, Schmitt F. The diagnostic and prognostic role of liquid-based cytology: are we ready to monitor therapy and resistance? Expert Rev Anticancer Ther. 2015;15(8):911–21.

    Article  CAS  PubMed  Google Scholar 

  107. Son C, Kang EJ, Roh MS. Strategic management of transthoracic needle aspirates for histological subtyping and EGFR testing in patients with peripheral lung cancer: an institutional experience. Diagn Cytopathol. 2015;43(7):532–8.

    Article  PubMed  Google Scholar 

  108. Wu HH, Jones KJ, Cramer HM. Immunocytochemistry performed on the cell-transferred direct smears of the fine-needle aspirates: a comparison study with the corresponding formalin-fixed paraffin-embedded tissue. Am J Clin Pathol. 2013;139(6):754–8.

    Article  CAS  PubMed  Google Scholar 

  109. Marshall AE, Cramer HM, Wu HH. The usefulness of the cell transfer technique for immunocytochemistry of fine-needle aspirates. Cancer Cytopathol. 2014;122(12):898–902.

    Article  PubMed  Google Scholar 

  110. Srebotnik Kirbis I, Us Krasovec M, Pogacnik A, Strojan Flezar M. Optimization and validation of immunocytochemical detection of oestrogen receptors on cytospins prepared from fine needle aspiration (FNA) samples of breast cancer. Cytopathol Off J Brit Soc Clin Cytol. 2015;26(2):88–98.

    Article  CAS  Google Scholar 

  111. Rossi ED, Fadda G, Schmitt F. The nightmare of indeterminate follicular proliferations: when liquid-based cytology and ancillary techniques are not a moon landing but a realistic plan. Acta Cytol. 2014;58(6):543–51.

    Article  CAS  PubMed  Google Scholar 

  112. Pegolo E, Machin P, Riosa F, Bassini A, Deroma L, Di Loreto C. Hormone receptor and human epidermal growth factor receptor 2 status evaluation on ThinPrep specimens from breast carcinoma: correlation with histologic sections determination. Cancer Cytopathol. 2012;120(3):196–205.

    Article  PubMed  Google Scholar 

  113. Domanski AM, Monsef N, Domanski HA, Grabau D, Ferno M. Comparison of the oestrogen and progesterone receptor status in primary breast carcinomas as evaluated by immunohistochemistry and immunocytochemistry: a consecutive series of 267 patients. Cytopathol Off J Brit Soc Clin Cytol. 2013;24(1):21–5.

    Article  CAS  Google Scholar 

  114. Ieni A, Barresi V, Reggiani Bonetti L, Branca G, Caruso RA, Tuccari G. Cytohistological and immunohistochemical characteristics of spindle-shaped mesenchymal neoplasms occurring in the gastrointestinal tract. Scand J Gastroenterol. 2017;52(3):291–9.

    Article  CAS  PubMed  Google Scholar 

  115. Vohra P, Buelow B, Chen YY, Serrano M, Vohra MS, Berry A, et al. Estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 expression in breast cancer FNA cell blocks and paired histologic specimens: a large retrospective study. Cancer Cytopathol. 2016;124(11):828–35.

    Article  CAS  PubMed  Google Scholar 

  116. Gailey MP, Bellizzi AM, Jensen CS. Differentiating small cell carcinoma from squamous cell carcinoma in cytologic specimens: a head-to-head comparison of p40 and p63 using cell block immunocytochemistry. Appl Immunohistochem Mol Morphol AIMM Off Publ Soc Appl Immunohistochem. 2016;24(1):11–5.

    Article  CAS  Google Scholar 

  117. Jalaly JB, Lewis JS Jr, Collins BT, Wu X, Ma XJ, Luo Y, et al. Correlation of p16 immunohistochemistry in FNA biopsies with corresponding tissue specimens in HPV-related squamous cell carcinomas of the oropharynx. Cancer Cytopathol. 2015;123(12):723–31.

    Article  CAS  PubMed  Google Scholar 

  118. Ieni A, Barresi V, Todaro P, Caruso RA, Tuccari G. Cell-block procedure in endoscopic ultrasound-guided-fine-needle-aspiration of gastrointestinal solid neoplastic lesions. World J Gastrointest Endosc. 2015;7(11):1014–22.

    Article  PubMed  PubMed Central  Google Scholar 

  119. Ibrahim TR, Abdel-Raouf SM. Immunohistochemical study of Glypican-3 and HepPar-1 in differentiating hepatocellular carcinoma from metastatic carcinomas in FNA of the liver. Pathol Oncol Res POR. 2015;21(2):379–87.

    Article  CAS  PubMed  Google Scholar 

  120. Collins BT, Garcia TC, Hudson JB. Effective clinical practices for improved FNA biopsy cell block outcomes. Cancer Cytopathol. 2015;123(9):540–7.

    Article  PubMed  Google Scholar 

  121. Hanley KZ, Birdsong GG, Cohen C, Siddiqui MT. Immunohistochemical detection of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 expression in breast carcinomas: comparison on cell block, needle-core, and tissue block preparations. Cancer. 2009;117(4):279–88.

    PubMed  Google Scholar 

  122. Wu M, Kafanas A, Gan L, Kohtz DS, Zhang L, Genden E, et al. Feasibility of immunocytochemical detection of tumor markers (XIAP, phosphohistone H1 and p63) in FNA cellblock samples from head and neck squamous cell carcinoma. Diagn Cytopathol. 2008;36(11):797–800.

    Article  CAS  PubMed  Google Scholar 

  123. Chandan VS, Faquin WC, Wilbur DC, Khurana KK. The role of immunolocalization of CD57 and GLUT-1 in cell blocks in fine-needle aspiration diagnosis of papillary thyroid carcinoma. Cancer. 2006;108(5):331–6.

    Article  PubMed  Google Scholar 

  124. Mitelman F, Johansson B, Mertens F. Mitelman database of chromosome aberrations and gene fusions in cancer 2017 [Available from: http://cgap.nci.nih.gov/Chromosomes/Mitelman].

  125. Heim S, Mitelman F. Nonrandom chromosome abnormalities in cancer: an overview. In: Heim S, Mitelman F, editors. Cancer cytogenetics: chromosomal and molecular genetic abberrations of tumor cells. 4th ed. Chichester: Wiley Blackwell; 2015. p. 26–41.

    Chapter  Google Scholar 

  126. Mitelman F, Johansson B, Mertens F. The impact of translocations and gene fusions on cancer causation. Nat Rev Cancer. 2007;7(4):233–45.

    Article  CAS  PubMed  Google Scholar 

  127. Antonescu CR. The GIST paradigm: lessons for other kinase-driven cancers. J Pathol. 2011;223(2):251–61.

    Article  CAS  PubMed  Google Scholar 

  128. Heim S, Mitelman F. Cancer cytogenetics : chromosomal and molecular genetic aberrations of tumor cells. 4th ed. Chichester: Wiley Blackwell; 2015. p. ix. 632 pages p.

    Book  Google Scholar 

  129. Swansbury J. Introduction. Cancer cytogenetics: methods and protocols. Methods Mol Biol. 2003;220:1–8.

    PubMed  Google Scholar 

  130. Bofin AM, Ytterhus B, Martin C, O’Leary JJ, Hagmar BM. Detection and quantitation of HER-2 gene amplification and protein expression in breast carcinoma. Am J Clin Pathol. 2004;122(1):110–9.

    Article  CAS  PubMed  Google Scholar 

  131. Lin F, Shen T, Prichard JW. Detection of Her-2/neu oncogene in breast carcinoma by chromogenic in situ hybridization in cytologic specimens. Diagn Cytopathol. 2005;33(6):376–80.

    Article  PubMed  Google Scholar 

  132. Zhang S, Abreo F, Lowery-Nordberg M, Veillon DM, Cotelingam JD. The role of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis and classification of lymphoproliferative disorders on fine-needle aspiration. Cancer Cytopathol. 2010;118(2):105–12.

    Article  PubMed  Google Scholar 

  133. Khalid A, Nodit L, Zahid M, Bauer K, Brody D, Finkelstein SD, et al. Endoscopic ultrasound fine needle aspirate DNA analysis to differentiate malignant and benign pancreatic masses. Am J Gastroenterol. 2006;101(11):2493–500.

    Article  CAS  PubMed  Google Scholar 

  134. Smith GD, Chadwick BE, Willmore-Payne C, Bentz JS. Detection of epidermal growth factor receptor gene mutations in cytology specimens from patients with non-small cell lung cancer utilising high-resolution melting amplicon analysis. J Clin Pathol. 2008;61(4):487–93.

    Article  CAS  PubMed  Google Scholar 

  135. Algeciras-Schimnich A, Milosevic D, McIver B, Flynn H, Reddi HV, Eberhardt NL, et al. Evaluation of the PAX8/PPARG translocation in follicular thyroid cancer with a 4-color reverse-transcription PCR assay and automated high-resolution fragment analysis. Clin Chem. 2010;56(3):391–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  136. Lappinga PJ, Kip NS, Jin L, Lloyd RV, Henry MR, Zhang J, et al. HMGA2 gene expression analysis performed on cytologic smears to distinguish benign from malignant thyroid nodules. Cancer Cytopathol. 2010;118(5):287–97.

    Article  CAS  PubMed  Google Scholar 

  137. De Biase I. Commentary. Clin Chem. 2016;62(8):1064.

    Article  PubMed  Google Scholar 

  138. Kameta E, Sugimori K, Kaneko T, Ishii T, Miwa H, Sato T, et al. Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing. Oncol Lett. 2016;12(5):3875–81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  139. Turbat-Herrera EA, Herrera GA. Electron microscopy renders the diagnostic capabilities of cytopathology more precise: an approach to everyday practice. Ultrastruct Pathol. 2005;29(6):475–82.

    Article  PubMed  Google Scholar 

  140. Taccagni G, Cantaboni A, Dell'Antonio G, Vanzulli A, Del Maschio A. Electron microscopy of fine needle aspiration biopsies of mediastinal and paramediastinal lesions. Acta Cytol. 1988;32(6):868–79.

    CAS  PubMed  Google Scholar 

  141. Davidson DD, Conces DJ, Goheen MP, Clark SA. Comparative ultrastructure of needle aspiration biopsy and surgical resection specimens of lung tumors. Ultrastruct Pathol. 1992;16(5):505–19.

    Article  CAS  PubMed  Google Scholar 

  142. Neill JS, Silverman JF. Electron microscopy of fine-needle aspiration biopsies of the mediastinum. Diagn Cytopathol. 1992;8(3):272–7.

    Article  CAS  PubMed  Google Scholar 

  143. Domanski HA. Elastic fibers in elastofibroma dorsi by fine-needle aspiration. Diagn Cytopathol. 2014;42(7):609–11.

    Article  PubMed  Google Scholar 

  144. Domanski HA, Akerman M, Rissler P, Gustafson P. Fine-needle aspiration of soft tissue leiomyosarcoma: an analysis of the most common cytologic findings and the value of ancillary techniques. Diagn Cytopathol. 2006;34(9):597–604.

    Article  PubMed  Google Scholar 

  145. Kindblom LG, Walaas L, Widehn S. Ultrastructural studies in the preoperative cytologic diagnosis of soft tissue tumors. Semin Diagn Pathol. 1986;3(4):317–44.

    CAS  PubMed  Google Scholar 

  146. Shidham VB, Hunt B, Jardeh SS, Barboi AC, Devata S, Hari P. Performing and processing FNA of anterior fat pad for amyloid. J Vis Exp. 2010(44):1747.

    Google Scholar 

  147. Walaas L, Kindblom LG. Light and electron microscopic examination of fine-needle aspirates in the preoperative diagnosis of osteogenic tumors: a study of 21 osteosarcomas and two osteoblastomas. Diagn Cytopathol. 1990;6(1):27–38.

    Article  CAS  PubMed  Google Scholar 

  148. Kilpatrick SR, editor. Diagnostic musculoskeletal surgical pathology. Clinicoradiologic and cytologic correlations. Philadelphia: Saunders; 2004.

    Google Scholar 

  149. Douville NJ, Bradford CR. Comparison of ultrasound-guided core biopsy versus fine-needle aspiration biopsy in the evaluation of salivary gland lesions. Head Neck. 2013;35(11):1657–61.

    Article  PubMed  Google Scholar 

  150. Pavan C, Parisi A, Girelli D. Recurrent needle-tract metastases of hepatocellular carcinoma following fine-needle aspiration. Intern Med J. 2007;37(2):134–6.

    Article  CAS  PubMed  Google Scholar 

  151. Ito Y, Asahi S, Matsuzuka F, Nakamura Y, Amino N, Miyauchi A. Needle tract implantation of follicular neoplasm after fine-needle aspiration biopsy: report of a case. Thyroid Off J Am Thyroid Assoc. 2006;16(10):1059–62.

    Article  Google Scholar 

  152. Ito Y, Tomoda C, Uruno T, Takamura Y, Miya A, Kobayashi K, et al. Needle tract implantation of papillary thyroid carcinoma after fine-needle aspiration biopsy. World J Surg. 2005;29(12):1544–9.

    Article  PubMed  Google Scholar 

  153. Slywotzky C, Maya M. Needle tract seeding of transitional cell carcinoma following fine-needle aspiration of a renal mass. Abdom Imaging. 1994;19(2):174–6.

    Article  CAS  PubMed  Google Scholar 

  154. Yamada N, Shinzawa H, Ukai K, Wakabayashi H, Togashi H, Takahashi T, et al. Subcutaneous seeding of small hepatocellular carcinoma after fine needle aspiration biopsy. J Gastroenterol Hepatol. 1993;8(2):195–8.

    Article  CAS  PubMed  Google Scholar 

  155. Tyagi R, Dey P. Needle tract seeding: an avoidable complication. Diagn Cytopathol. 2014;42(7):636–40.

    Article  PubMed  Google Scholar 

  156. Shields CL, Manquez ME, Ehya H, Mashayekhi A, Danzig CJ, Shields JA. Fine-needle aspiration biopsy of iris tumors in 100 consecutive cases: technique and complications. Ophthalmology. 2006;113(11):2080–6.

    Article  PubMed  Google Scholar 

  157. Shah KS, Ethunandan M. Tumour seeding after fine-needle aspiration and core biopsy of the head and neck – a systematic review. Br J Oral Maxillofac Surg. 2016;54(3):260–5.

    Article  PubMed  Google Scholar 

  158. Roussel F, Dalion J, Benozio M. The risk of tumoral seeding in needle biopsies. Acta Cytol. 1989;33(6):936–9.

    CAS  PubMed  Google Scholar 

  159. Smith EH. Complications of percutaneous abdominal fine-needle biopsy. Rev Radiol. 1991;178(1):253–8.

    Article  CAS  Google Scholar 

  160. Gomez-Rubio M, Lopez-Cano A, Rendon P, Munoz-Benvenuty A, Macias M, Garre C, et al. Safety and diagnostic accuracy of percutaneous ultrasound-guided biopsy of the spleen: a multicenter study. J Clin Ultrasound JCU. 2009;37(8):445–50.

    Article  PubMed  Google Scholar 

  161. Virgilio E, Mercantini P, Ferri M, Cunsolo G, Tarantino G, Cavallini M, et al. Is EUS-FNA of solid-pseudopapillary neoplasms of the pancreas as a preoperative procedure really necessary and free of acceptable risks? Pancreatol Off J Int Assoc Pancreatol. 2014;14(6):536–8.

    Article  Google Scholar 

  162. Vadvala HV, Furtado VF, Kambadakone A, Frenk NE, Mueller PR, Arellano RS. Image-guided percutaneous omental and mesenteric biopsy: assessment of technical success rate and diagnostic yield. J Vasc Interv Radiol JVIR. 2017;28(11):1569–76.

    Article  PubMed  Google Scholar 

  163. Marconi L, Dabestani S, Lam TB, Hofmann F, Stewart F, Norrie J, et al. Systematic review and meta-analysis of diagnostic accuracy of percutaneous renal tumour biopsy. Eur Urol. 2016;69(4):660–73.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Henryk A. Domanski .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Domanski, H.A., Mertens, F. (2019). Introduction. In: Domanski, H. (eds) Atlas of Fine Needle Aspiration Cytology. Springer, Cham. https://doi.org/10.1007/978-3-319-76980-6_1

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-76980-6_1

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-76979-0

  • Online ISBN: 978-3-319-76980-6

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics