The Analytical Evaluation of Acrylamide in Foods as a Maillard Reaction Product

  • Rajeev K. SinglaEmail author
  • Ashok K. Dubey
  • Sara M. Ameen
  • Shana Montalto
  • Salvatore Parisi
Part of the SpringerBriefs in Molecular Science book series (BRIEFSMOLECULAR)


From the sanitary viewpoint, acrylamide has been repeatedly considered as a toxic compound in foods. Actually, the toxicological importance of acrylamide cannot be discussed without reference to food browning phenomena and ‘Maillard reaction’, a series of cascade reactions. The most visible effect of Maillard reaction in thermally treated foods and beverages in certain processed foods is undoubtedly the non-enzymatic browning effect. In addition, certain questioned products can be obtained, including acrylamide. This molecule has been found in bakes, fried, and toasted products with notable amounts. The most probable reason for acrylamide production in these situations is the reaction of certain amino acids such as asparagine with reducing sugars. Because of the toxicological importance ascribed to acrylamide, this chapter is completely dedicated to this molecule and related analytical methods concerning food matrices.


Acrylamide Amino compound Browning effect Chromatography Maillard reaction Melanoidins Reducing sugar 



Capillary zone electrophoresis


European Food Safety Authority


EFSA Panel on Contaminants in the Food Chain


Gas chromatography


Gas chromatography/mass spectrometry


High-performance liquid chromatography


High-performance liquid chromatography/mass spectrometry


HPLC-tandem mass spectrometry




Maillard reaction product


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Copyright information

© The Author(s) 2018

Authors and Affiliations

  • Rajeev K. Singla
    • 1
    Email author
  • Ashok K. Dubey
    • 1
  • Sara M. Ameen
    • 2
  • Shana Montalto
    • 3
  • Salvatore Parisi
    • 4
  1. 1.Division of Biological Sciences and EngineeringNetaji Subhas Institute of TechnologyDwarkaIndia
  2. 2.Medical Research Laboratories, Faculty of ScienceHelwan UniversityCairoEgypt
  3. 3.Food Safety ConsultantFloridiaItaly
  4. 4.Industrial ConsultantPalermoItaly

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