Abstract
Cathepsins are proteolytic enzymes with a broad spectrum of substrates. They are known to reside within endo-lysosomes where they acquire optimal conditions for proteolytic activity and substrate cleavage. However, cathepsins have been detected in locations other than the canonical compartments of the endocytotic pathway. They are often secreted from cells in either proteolytically inactive proform or as mature and active enzyme; this may happen in both physiological and pathological conditions. Moreover, cytosolic and nuclear forms of cathepsins have been described and are currently an emerging field of research aiming at understanding their functions in such unexpected cellular locations. This chapter summarizes the canonical pathways of biosynthesis and transport of cathepsins in healthy cells. We further describe how cathepsins can reach unexpected locations such as the extracellular space or the cytosol and the nuclear matrix. No matter where viruses and cathepsins encounter, several outcomes can be perceived. Thus, scenarios are discussed on how cathepsins may support virus entry into host cells, involve in viral fusion factor and polyprotein processing in different host cell compartments, or help in packaging of viral particles during maturation. It is of note to mention that this review is not meant to comprehensively cover the present literature on viruses encountering cathepsins but rather illustrates, on some representative examples, the possible roles of cathepsins in replication of viruses and in the course of disease.
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Keywords
- Aspartic cathepsins
- Cysteine cathepsins
- Serine cathepsins
- Nuclear cathepsins
- Trafficking
- Activity-based probes
- Green fluorescent protein
- Substrate cleavage
- Protease inhibition
1 Biosynthesis and Default Trafficking Pathway
Cathepsins are synthesized as preproenzymes, whereby the signal peptide targets for entry of the nascent chains into the lumen of the rough endoplasmic reticulum (rER). The signal peptide (pre) is co-translationally cleaved off by signal peptidase upon entry into the ER lumen. The propeptides (pro) keep p rocathepsins in the zymogen form, that is, proteolytically inactive during their transport from the rER via the Golgi apparatus and the trans-Golgi network (TGN) to late endosomes (Fig. 10.1) (Brix et al. 2008, 2015; Erickson et al. 2013). Procathepsins need to pass the quality control of the rER before they become posttranslationally modified while traveling to the TGN. Interestingly, proper folding of cathepsins can be conveyed by their propeptides as some of these bear chaperone functions. The most important and best-studied posttranslational modification of cathepsins is mannose 6-phosphorylation. It is believed to occur in the cis-Golgi and to be required for recognition by cation-dependent mannose 6-phosphate receptors (CD-MPR) of the TGN, which sort the zymogens into clathrin-coated transport vesicles (Fig. 10.1) (De Duve and Wattiaux 1966; Kornfeld and Mellman 1989; von Figura 1991; Kornfeld 1992; Erickson et al. 2013; Brix et al. 2015). Upon arrival in late endosomes, the pH drops such that procathepsins dissociate from the sorting CD-MPR, which is recycled back to the TGN via retromer-coated vesicles. In late endosomes, procathepsins are proteolytically processed to acquire the mature and proteolytically active state (Fig. 10.1). Therefore, either asparaginyl endo peptidase (AEP), which is better known as legumain, or cathepsins themselves cleave procathepsins in trans such that the propeptides are removed and the mature forms are delivered for further functioning as soluble enzymes in the compartments of the endocytic pathway. Thus, proteolytic processing for maturation and activation of procathepsins takes place in the late endosome.
As indicated above, the molecul ar architecture of cathepsins features N-terminal signal peptides that are typically followed by inhibitory propeptides and the peptidase domains (Tables 10.1, 10.2 and 10.3). However, the mature, single-chain form of cathepsin B, in particular, can be processed further, thereby yielding a two-chain form that consists of a light and a heavy chain, which remain bound to each other by disulfide bonds (Mort and Buttle 1997). Both, single- and two-chain forms of cathepsin B are proteolytically active as hydrolases. The molecular architecture of procathepsin-activating AEP/legumain differs from that of cathepsins, in that the peptidase domain directly follows the signal peptide and the pro-domain is found at the C-terminus (Table 10.2). Legumain is further exceptional, because it acts not only as a peptidase but features also peptide lig ase activity depending on the conditions it is exposed to (Dall and Brandstetter 2016).
2 Proteolytic Activity and Substrate Cleavage Preferences of Aspartic, Cysteine, and Serine Cathepsins
Cathepsins belong to either of three classes of proteolytic enzymes, namely, aspartic, cysteine, or serine proteases (Rawlings 2013; Rawlings et al. 2016). Hence, cathepsins are classified according to the amino acids of their active sites that are responsible for catalytic hydrolysis of peptide bonds. Aspartic acid or cysteine residues are part of the catalytic dyad of the aspartic and cysteine cathepsins , respectively, while serine is part of a catalytic triad in which an additional residue is required to stabilize the oxyanion hole in the acyl intermediate upon interaction between the enzyme and its substrate. Aspartic cathepsins are acting as water nucleophiles, whereas cysteine and serine cathepsin s act as protein nucleophiles (Baici et al. 2013). Hence, the nucleophile is provided by a side chain of an amino acid in the active site, namely, the sulfhydryl or the hydroxyl group of either cysteine or serine, respectively, in cysteine and serine cathepsins. For example, serine bears the nucleophilic hydroxyl group of serine cathepsins, while histidine acts as the general base. In addition, aspartate helps to orient the imidazolium ring of histidine such that it activates serine to perform the nucleophilic attack on the peptide bond of the substrate, whereby a temporary complex between the enzyme and its substrate forms, i.e., the acyl intermediate. This breaks down rapidly, resulting in protonation of the general base histidine. Subsequent hydrolysis of the scissile bond occurs when a water molecule enters. The reaction mechanism of peptide bond hydrolysis catalyzed by cathepsins therefore involves two substrates, the protein or peptide substrate and a water molecule, and two products are generated, na mely, an N- and a C-terminal peptide product.
Most of the cathepsins act as endopeptidases (Fig. 10.2). However, the cysteine cathepsins B and X, the serine cathepsin A, and legumain are also acting as carboxypeptidases, whereas cysteine cathepsin H acts as an aminopeptidase and cysteine cathepsin C forms dimers acting as dipeptidyl peptidase (Fig. 10.2). Thus, cathepsins are mostly acting on their peptide or protein substrates as monomers, but some can dimerize or even multimerize, thereby eventually altering substrate specificity (see also below).
Proteolytic activity and substrate specificity are typically determined with recombinant enzyme preparations in well-defined in vitro assays. According to the Schechter and Berger nomenclature (Schechter and Berger 1968), the amino acids next to the scissile bond toward the N-terminus of the substrate are named P1, P2, P3, and P4, while the C-terminal amino acid next to the scissile bond is named P1′, and the following amino acids of the substrate are referred to as P2′, P3′, and P4′, respectively. The substrate-binding pockets in the cathepsins are named accordingly as S1–S4 and S1′–S4′, respectively, as for every other peptidase. Standard assays to determine the activity of cathepsins are available, as are synthetic substrates, which are preferentially or specifically cleaved by the enzymes. Standard cathepsin activity assays mainly consider peptide cleavage at optimal conditions, whereby a reducing environment of acidic to slightly acidic pH—mimicking the conditions in the endo-lysosomal compartments—is considered as the main determinant. The reader is referred to the Handbook of Proteolytic Enzymes (Barrett 2004) and to the “MEROPS database” (www.merops.sanger.ac.uk) (Rawlings et al. 2016), which provides a comprehensive and excellent compendium of proteases, their substrates, and inhibitors, for further up-to-date information.
It should be noted that cathepsins vary in their substrate specificities, but many exhibit overlapping substrate cleavage preferen ces, making it sometimes difficult to distinguish cathepsin activities. This ambiguity in substrate specificity often complicates their specific inhibition. In addition, cathepsins are redundantly expressed (Brix et al. 2008; Reiser et al. 2010; Gansz et al. 2013; Sloane et al. 2013). Thus, a cathepsin can be upregulated to take over the function of a related enzyme, when the latter is eliminated by gene knockout or inhibited by pharmacological interventions. This phenomenon of redundant regulation of cathepsin expression is observed especially when covalent and irreversible instead of transient reversible inhibition is applied.
3 Endogenous Inhibitors of Cathepsins
Cathepsin activities are counterbalanced by endogenous inhibitors (Tables 10.1, 10.2 and 10.3). The aspartic cathepsins D an d E, which are mostly detected in lysosomes, are inhibited by pepstatin (Dean 1979) or by a natural product isolated from the sea anemone Actinia equina, equistatin. This latter inhibitor interestingly also interacts with cysteine peptidases (Lenarcic et al. 1997; Lenarcic and Turk 1999) including the cysteine cathepsins that are found preferentially in endo-lysosomes but also frequently in extra- and pericellular locations. Furthermore, aberrant or alternative forms of cysteine cathepsins are detected in the cytosol, as well as the nuclear and mitochondrial matrices (Brix et al. 2015). Cytosolic cystatins A and B, also known as stefins A and B (Machleidt et al. 1983; Brzin et al. 1983), as well as secreted cystatins C, D, E/M, F, and SN (Barrett 1986; Sloane et al. 1990; Turk and Bode 1991; Alvarez-Fernandez et al. 1999; Abrahamson et al. 2003; Turk et al. 2008; Zeeuwen et al. 2009) serve as endogenous inhibitors of the cysteine cathepsins. In addition, alpha-2-macroglobulin has been identified as an inhibitor of cysteine cathepsins (Fritz 1979; Travis 1988). Cysteine catheps ins, in principle, can also be inhibited by serpins, which are cross-class inhibitors of serine proteases (Silverman et al. 2001). Like the cystatins, serpins are present intra- and extracellularly. Serpins have a reactive center loop that serves as a bait for the attacking serine protease and, upon cleavage, remains bound by covalent linkage to the enzyme (Huntington 2006; Whisstock et al. 2010).
4 Tools to Study Expression, Trafficking, and Activities of Cathepsins
Numerous tools are available to study the cathepsins. Specific antibodies are used in immunostaining experiments for subsequent inspection by light and electron microscopy. Such strategies in using antibodies for the detection of cathepsins often bear specificity problems (Weber et al. 2015), because cathepsins are evolutionary conserved and show extensive sequence homologies.
Synthetic substrates, like chromogenic or fluorogenic peptides , are available for in vitro activity assays (see, MEROPS database for further details). Likewise, cyto- or histochemical approaches have been described, which employ synthetic cathepsin substrates that are converted into products less diffusible and readily detected by means of microscopy (Spiess et al. 1994; Brix et al. 1996). Enzymography in polyacrylamide gels is another means of determining multiple cathepsin activities at a time in cell or tissue lysates (Chen and Platt 2011; Platt et al. 2016).
A number of chimeric proteins consisting of cathepsins and fluorescent proteins have been generated which can be expressed in mammalian cells (for reviews, see Brix et al. 2008; Arampatzidou et al. 2011). Tagged cathepsins follow the regular transport pathways like the endogenous enzymes (Naganawa et al. 1999; Linke et al. 2002a, b; Arampatzidou et al. 2011; Frizler et al. 2013; Tamhane et al. 2015; Tamhane et al. 2016). Trafficking studies in living cells have been combined with co-localization experiments in which compartment-specific markers have been used to spot full-length or N-terminally truncated specific forms of cathepsins (see below) while they are on the move through the cell (Linke et al. 2002a; Zwicky et al. 2003; Müntener et al. 2004; Mayer et al. 2008). Intervention by either permanently blocking or transiently interfering with specific transport routes has been applied in order to investigate distinct enzyme trafficking in a variety of eukaryotic cells.
The most elegant way to visualize cathepsin activities “on the spot” and in a quantitative manner is by using so-called activity-based probes (ABPs) (Greenbaum et al. 2002; Jessani et al. 2004; Blum et al. 2005; Blum 2008; Edgington et al. 2011; Blais et al. 2012; Zou et al. 2012; Grzywa and Sieńczyk 2013; Sanman and Bogyo 2014). ABPs consist of a reactive functional group (so-called warhead) that binds to the target protease, a linker region that resembles a peptide substrate, and additional tagging groups. The tagging groups are typically fluorogenic, but biotin or iodinatable moieties have also been chemically engineered onto ABPs . Hence, a family or subfamilies of cathepsins are functionally addressed and become covalently tagged upon cleavage of the ABP’s linker. ABPs are available as broad-spectrum probes used in approaching the proteolytic activities of aspartic, cysteine, and serine cathepsin s. In addition, specific ABPs have been synthesized that can be cleaved by one or only few cathepsins. Moreover, ABPs with propeptide-mimicking features in the peptide backbone have been used (Schaschke et al. 1998; Diederich et al. 2012). Such highly specific ABPs are, in principle, able to distinguish even closely related cathepsins.
The one-to-one binding principle of ABPs renders them powerful tools to quantify cathepsin activities. This is to say that most ABPs act as inhibitors that are attacked and cleaved in a substrate-like fashion, but remain bound to the targeted enzyme, thus forming an irreversible, covalent complex with the target protease. The concept of ABP labeling of cathepsins can be compared with the suicidal inhibition mechanism of the serpins on their target proteases (see above). The principle of specific and irreversible binding bears the advantage of rendering ABPs into quantitative probes that allow to detect only mature, proteolytically active cathepsins. However, the disadvantage of many ABPs is equally intrinsic, namely, they act as protease inhibitors and may eventually lead to pharmacological knockdown of proteolytic activities when applied to living cells.
The above sketched labeling options to visualize, localize, and quantify cathepsins can be and have been combined in all possible variations (Baruch et al. 2004; Blum et al. 2005, 2007; Brix and Jordans 2005; Blum 2008; Sadaghiani et al. 2007; Brix et al. 2008; Arampatzidou et al. 2011; Salpeter and Blum 2013). Such experiments have helped to uncover the transport pathways that are followed by pro- and mature cathepsins in different eukaryotic cells. Importantly, it is now state of the art to distinguish proteolytically active from inactive cathepsins. Moreover, it became clear that each and every cell type may use cathepsins strategically in its own, distinct way in support of the specific cell’s function (Brix et al. 2008, 2015; Mohamed and Sloane 2006; Sloane et al. 2013; Weiss-Sadan et al. 2017). Hence, subcellular locations of cathepsin activities have been detected that encompass the expected canonical endo-lysosomal compartments. However, quite often locations of cathepsin activities were detected which were, by all means, unexpected. Thus, cathepsins exhibit an astonishing variety of transport pathways to reach numerous intra- and extracellular locations where the proteases may function in a spatially confined and temporally regulated manner (Mohamed and Sloane 2006; Gocheva and Joyce 2007; Brix et al. 2013, 2015; Akkari et al. 2016).
5 Unexpected Locations Reached by Cathepsins Are Explained by Noncanonical Trafficking
5.1 Alternative Pathways of Activation upon Secretion of Procathepsins
Procathepsins may skip recognition by the CD-MPR at the TGN and become further sorted and transported along the secretory pathway with destination to the cell surface for subsequent secretion into the extracellular space (Fig. 10.1, left transport route). Pericellularly, the procathepsins may become activated by soluble endopeptidases or through the action of plasma membrane-bound ectoenzymes. Yet another pathway is used in particular in cells of the innate immune system, like macrophages, that express the cation-independent mannose 6-phosphate receptor (CI-MPR, also referred to as IGFII/M6PR) (Mason et al. 1987; Pohlmann et al. 1995; Collette et al. 2004). This cell surface receptor acts as a scavenger receptor and functions in rescuing faulty or excessively secreted procathepsins for subsequent internalization by endocytosis and sorting along the endocytic pathway. Hence, during their transport from the extracellular space via early endosomes to late endosomes (Fig. 10.1, right transport route), such secreted forms of cathepsins eventually are activated in the same compartment as those following the default transport pathway but delayed in time.
5.2 Secretory Lysosomes
Distinct cell types feature so-called secretory lysosomes (Andrews 2000; Brix et al. 2008). These are endocytic compartments, which resemble late endosomes and lysosomes in their characteristic biochemical composition. Hence, secretory lysosomes or cathepsins recruited from within late endosomes and lysosomes are transported in a retrograde fashion, such that the vesicles fuse with the plasma membrane, typically upon signaling. This alternative route of cathepsin transport (Fig. 10.1, bottom right transport route) is observed in a number of specific cell types and allows for secretion of proteolytically active cathepsins into the extracellular space (Linke et al. 2002a, b; Büth et al. 2004).
5.3 Cathepsins in Extracellular Locations and Bound to the Cell Surface
Cathepsins are present in the extracellular space as mature and active enzymes or as inactive proforms. While procathepsins that underw ent mannose 6-phosphorylation bind to the CI-MPR (see above) (von Figura 1991; Erickson et al. 2013; Brix et al. 2015), mature cathepsins and procathepsins can also become bound by alpha-2-macroglobulin (Arkona and Wiederanders 1996; Peloille et al. 1997). Moreover, this protein serves as a regulator of a number of proteases, namely, it is an inhibitor of matrix metalloproteinases and cysteine cathepsins alike. Other receptors known to interact with secreted cathepsins are those of the diverse low-density lipoprotein (LDL) receptor-like protein (LRP) family of transmembrane proteins present at the cell surface of almost all cell types (Poller et al. 1995; Willnow et al. 1996; Herz and Strickland 2001). Megalin/gp330 is one of the LRPs that is supposed to mediate internalization and endo-lysosomal delivery of cathepsins, if previously secreted by mistake (Nielsen et al. 2007). Thus, LRPs function in a way similar to the CI-MPR but interact also with pro- and mature cathepsins lacking m annose 6-phosphorylation (Fig. 10.1, right transport route).
5.4 Regulation of Cathepsin Activities in the Extracellular Space
Pericellular and extra cellular localization of procathepsins and mature cathepsins has been observed under both physiological and pathological conditions. For instance, cathepsin-mediated extracellular proteolysis has been shown to promote cancer cell invasion and tumor progression through extracellular matrix (ECM) degradation (Rochefort et al. 2000; Joyce and Hanahan 2004; Liaudet-Coopman et al. 2006; Mohamed and Sloane 2006; Gocheva and Joyce 2007; Sloane et al. 2013). It remains to be seen, however, if the enhanced cathepsin secretion responsible for excessive ECM degradation is triggered by the acidic environment in tumor tissue or by other mechanisms (Cavallo-Medved and Sloane 2003).
Depending on the extracellular conditions, cathepsins may acquire altered or additional substrate preferences. For exam ple, the cysteine cathepsin K is secreted under physiological conditions as an active enzyme and acts in the acidic resorption lacuna formed by osteoclasts during bone turnover (Gelb et al. 1996; Saftig et al. 1998; Rachner et al. 2011; Fonović and Turk 2014; Brömme et al. 2016). However, cathepsin K is also responsible for degradation of thyroglobulin in the extracellular thyroid follicle lumen, namely, at neutral pH and in oxidizing conditions (Tepel et al. 2000; Friedrichs et al. 2003; Jordans et al. 2009). Moreover, secreted cathepsin K monomers can interact with each other, thereby forming polymeric ringlike structures, depending on the molecular composition of the extracellular space (Li et al. 2002). It has been shown that ECM components like glycosaminoglycans and other constituents are essential as scaffolding factors supporting extracellular interactions of cathepsin K molecules (Li et al. 2004). Moreover, cathepsin K can be stabilized extracellularly by binding to cell surface molecules like clusterin (Novinec et al. 2012), which also interacts with protease receptors of the LRP family, e.g., megalin/gp330 (Lemansky et al. 1999). Importantly, the activity of this particular enzyme—cysteine cathepsin K—differs in monomeric and dimeric as well as in soluble and ECM- or membrane-bound form (Aguda et al. 2014). Hence, different substrates can be cleaved by the same cathepsin, and a given substrate can be processed differently by that enzyme, depending on whether the cathepsin is secreted as an active monomer or dimer, scaffolded by ECM constituents, or bound by cell surface receptors complexed with allosteric cofactors. It is important to note that cysteine cathepsin K is well-studied, in particu lar, because of its significance as an anti-osteoporotic drug target.
These examples highlight that more research is required to understand how the enzymatic activities of cathepsins are regulated, in particular, in unexpected locations. Thus, the well-accepted concept that cathepsins optimally cleave protein and peptide substrates at acidic pH in reducing environments, as found in endo-lysosomes, must be broadened. This is all the more important when cathepsin activities are investigated under conditions of cellular stress as is the case with viral infection.
5.5 Cytosolic and Nuclear Cathepsins
As detailed above, cat hepsins belong to the obligate constituencies of the compartments of the endocytic pathway where they exert their functions most optimally. However, the phenomenon of leaky lysosomes has also been known for long. Cellular stress as is the case with cancer cells under prolonged drug treatment or UV irradiation can cause leakiness of endo-lysosomal membranes. Mature cathepsins may then be released into the cytosol, where proteolytic activity is controlled by different means, such as endogenous inhibitors that specifically bind to the enzymes or other biochemical properties of the cytosol that are not optimal for cathepsin-mediated cleavage. However, when escaping these safeguarding measures by, for instance, inhibitor downregulation, proteolytically active cathepsins may be present in the cytosol for prolonged time intervals and induce apoptotic, necrotic, or pyroptotic cascades resulting in cell death (Turk et al. 2000; Luke et al. 2007; Turk and Turk 2009; Aits and Jäättelä 2013; Flütsch and Grütter 2013). Fu rthermore, cytosolic and nuclear cathepsins may modulate cell cycle progression (Goulet and Nepveu 2004; Brix et al. 2015; Tamhane et al. 2016).
5.6 Alternative Cathepsin Forms
N-terminally truncated for ms of the canonical preprocathepsins translated from, e.g., alternative transcripts are believed to lack the signal peptide and parts of the propeptide (Mehtani et al. 1998; Zwicky et al. 2003; Müntener et al. 2004; Baici et al. 2006; Schilling et al. 2009; Tholen et al. 2014; Brix et al. 2015). They are therefore not targeted for entry into the ER lumen and will not follow the secretory pathway. Instead, N-terminally truncated cathepsins are retained in the cytosol and can even fold properly as they acquire a proteolytically active state in this unexpected location (Goulet and Nepveu 2004; Luke et al. 2007; Reiser et al. 2010; Tedelind et al. 2010). Some of these alternative and aberrant forms of the cathepsins occur even in the nuclear matrix (Fig. 10.1, center at bottom). The mechanism by which they are transported through the nuclear pore complexes is not known, because most endo-lysosomal enzymes (except AEP/legumain) lack a nuclear localization sequence (NLS) . It is reasonable to assume, however, that such nuclear cathepsins, as well as the cytosolic forms, are involved in processing of transcription factors, core, and/or linker histones. In vitro experiments further revealed an important role of DNA as a potential scaffolding factor that interferes with serpin-mediated control of cathepsin activities in environments that mimic unexpected cellular locations like the nuclear matrix (Ong et al. 2007).
6 Cathepsins Meeting Viruses, Viruses Meeting Cathepsins
6.1 Transient Encounters when Traveling Along the Endocytic Pathway
Cathepsins are well known to pr ocess the spike proteins of SARS and MERS coronaviruses, thereby activating viral fusogens and enabling host cell entry from within endosomes (Millet and Whittaker 2015; Simmons et al. 2013; Heald-Sargent and Gallagher 2012). Thus, treatment options of preventing host cell infection with SARS-CoV have been proposed that involve cysteine cathepsin L inhibitors (Tong 2006).
A very complex mechanism of proteolytic activation of the fusion proteins of henipaviruses (HNV) has been described as an essential prerequisite for infectivity and pathogenicity of these highly pathogenic paramyxoviruses (Weis and Maisner 2015). The non-fusogenic F0 protein o f HNV is translated in host cells and is subsequently transported along the secretory pathway in its inactive form to the plasma membrane. Upon re-internalization and processing in recycling endosomes by cathepsins B and L (Pager and Dutch 2005; Meulendyke et al. 2005; Vogt et al. 2005; Pager et al. 2006; Diederich et al. 2005, 2012), activated F1/F2 complex travels back to the plasma membrane, where it is incorporated into budding virus particles, or mediates fusion of an infected cell with a neighboring cell. Hence, endosomal cysteine cathepsins B and L play an essential role in promoting spread of infection and formation of syncytia.
Thus, there are significant dif ferences in cathepsin-mediated activation of viral fusion proteins: with HNV it occurs at a late stage of replication, whereas SARS and MERS coronaviruses are activated upon virus entry into host cells. Endosomal cysteine cathepsins B and L are also involved in the processing of the envelope glycoproteins of Marburg and Ebola viruses. Cathepsin cleavage enables the glycoprotein to interact with the Niemann-Pick disease type C1 (NDC-1) protein of the host cell, which is an essential step in filovirus entry (Hunt et al. 2012). Likewise, endosomal cathepsins are utilized by non-enveloped reoviruses for host cell entry. After removal of the outer capsid protein σ3 by cathepsins, the viral protein μ1 is exposed, which is a fusion protein, promoting endos omal membrane rupture (Danthi et al. 2010).
6.2 Altered Cathepsin Expression in Virus-Infected Cells
Virus infection may affect transcriptional regulation of cathepsin genes leading to disbalanced cell functions. In addition to the N-terminally truncated forms of cathepsins and those reaching the cytosol as full-length enzymes due to release from endo-lysosomes, it is conceivable that alternate cathepsin forms may derive from altered genes. These may result from gene mutations or chromosomal aberrations, as occurring in cancer cells, or due to upregulated translation of alternative transcripts. Viral oncogenes may affect amplification of cathepsin genes (Mohamed and Sloane 2006), and it was proposed that endogenous retroviruses or elements thereof may activate placenta-specific genes encoding cysteine cathepsins of mice (Rawn and Cross 2008).
Moreover, HIV-infected macrophages have been reported to upregulate both cytosolic cysteine protease inhibitor cystatin B (stefin B) and cysteine cathepsin B, believed to trigger neuronal cell death in HIV-1-associated neurocognitive disorder (HAND) (Rivera et al. 2014). Similarly, virus transformation of cultured cells in vitro is known to cause upregulation and secretion of the so-called major excreted protein (MEP) , which was found in the secretion media of transformed fibroblasts and identified as proteolytically active cathepsin L (Mason et al. 1987; Rubin 2005). Thus, viruses may not only affect transcriptional regulation of cathepsin genes but may thereby also cause mis-trafficking of the proteases. In hepatocellular carcinoma, caused by infection with hepatitis B and C viruses, altered cathepsin trafficking also results in their secretion. In this particular case of virus-induced liver cancer, the over-secretion of cathepsins is further complicated by the defective functioning of the IGFII/M6P receptor (CI-MPR) pathway, normally acting as a recapture mechanism for internalizati on of faulty and excessively secreted cathepsins (see above) (Scharf and Braulke 2003). Thus, in the absence of re-internalization cues, the cathepsins may be present in enhanced amounts at the cell surface of hepatocellular carcinoma cells.
The findings summarized above show that another, so far only rarely considered scenario may be likely. Namely, viruses may interact with cathepsins present in the extracellular space of, e.g., cancer cells, even before entering the host cell’s endocytic compartments. In acknowledging that cathepsins may well act as proteolytic enzymes already in the pericellular space (see above), it becomes clear at this point that some viruses and their constituents can be processed, in principle, by extracellular cathepsins, that is, before actually entering the host cell by endocyto sis (Fig. 10.1, top, center).
It has also to be mentioned in this context that extracellular cathepsin B-mediated shedding of constituents of the glycocalyx of endothelial cells was proposed as a process in support of infection with viruses causing hemorrhagic fevers (Becker et al. 2015). Likewise, cysteine cathepsins B-, L-, and S-mediated shedding of E-cadherin, an important cell-cell adhesion molecule, was suggested to cause epit helial cell damage, thereby promoting disease progression in patients with viral infections (Grabowska and Day 2012).
Taken together, these observations illustrate different mechanisms by which viruses may upregulate expression and stimulate secretion of cathepsins. They also suggest that cathepsins activate viruses not only in endocytotic compartments but also at the cell surface. Finally, these findings support the c oncept that cathepsins contribute to pathogenesis not only by activating the fusion capacity of viruses but also by other mechanisms promoting cell and tissue damage.
6.3 Endo-Lysosomal Cathepsins and the Immune Response to Viral Infections
Viruses entering host cell s by endocytosis are known to trigger a Toll-like receptor (TLR)-mediated immune response which eventually leads to interferon-alpha production (Sun et al. 2010). Hence, viral nucleic acids are recognized as PAMPs (pathogen-associated molecular patterns) by proteolytically processed transmembrane pattern recognition receptors like TLR9. Proteolytic activation of TLR9 is catalyzed by endosomal AEP/legumain and cathepsins (Bauer 2013). Thus, cathepsins are also involved in the immune response to viral infection.
Cathepsin-mediated processing of viral proteins—typically protein fusogens—happens in early and recycling endosomes (see above), which are connected with other compartments of the endocytic pathway, namely, also with late endosomes or multivesicular bodies (MVBs). MVBs are believed to serve as a place for generation of exosomes (Cocucci and Meldolesi 2015; Hurley 2015). In this regard, it is interesting to note that infection with the filoviruses EBOV and MARV involves the molecular machinery of the ESCRT pathway, which is required for MVB formation and which is i mportant for virus replication, nucleocapsid formation, and maturation in a compartment positive for late endosomal markers (Dolnik et al. 2015).
Another vicious cycle is working in cross-presentation, which follows from initial phagocytosis of portions or entire virus-infected cells by dendritic cells (Rock and Shen 2005). These professional antigen-presenting cells depend on cysteine cathepsin S-mediated endosomal processing of internalized proteins, which then results in antigen presentation in the context of MHC class II. In the unfortunate case of endosomal processing of viral proteins, the resulting viral antigens may t herefore be presented on the surface of dendritic cells via MHC class II, instead of MHC class I. When antigen presentation happens in the context of MHC class I, alerted cytotoxic T cells eliminate the virus-infected cells. Upon cross-presentation, however, tolerance may be a nonproductive outcome.
Concluding Remarks
Finally, a chapter on cathepsins in a book on viruses cannot end without placing a special note in the context of therapeutic approaches aiming at eliminating viruses from the host. In particular, the retroviral HIV protease is important for maturation of the virus particles and, hence, targeted therapeutically (Moyle and Gazzard 1996; Cooper 2002). HIV proteinase structurally resembles the ubiquitously expressed aspartic cathepsin D, denoting the similarities between host cell’s aspartic cathepsins and retroviral aspartic proteases, which are—in the case of HIV—successfully inhibited when approached by transition-state inhibitors. Therefore, and in conclusion, basic science researchers, virologists, and clinicians have learned a lot from the structural similarities of host cell cathepsins and viral proteases. We deduce that interactions between cell biologists and virologists bear more interesting facts to be gathered in the future. Moreover, many more potential therapeutic answers are to be developed from the encounters of cathepsins and the various viruses utilizing the proteases in their own favor. Hence, some of the comments in this chapter are meant in support of stimulating future discussions in the spirit of thinking “out of the box,” and, like the cathepsins, following paths beyond the canonical pathways. In our opinion, this is an endeavor worth to be undertaken and continued in the future.
References
Abrahamson M, Alvarez-Fernandez M, Nathanson CM. Cystatins. Biochem Soc Symp. 2003;70:179–99.
Aguda AH, Panwar P, Du X, Nguyen NT, Brayer GD, Brömme D. Structural basis of collagen fiber degradation by cathepsin K. Proc Natl Acad Sci U S A. 2014;111(49):17474–9.
Aits S, Jäättelä M. Lysosomal cell death at a glance. J Cell Sci. 2013;126(Pt 9):1905–12.
Akkari L, Gocheva V, Quick ML, Kester JC, Spencer AK, Garfall AL, Bowman RL, Joyce JA. Combined deletion of cathepsin protease family members reveals compensatory mechanisms in cancer. Genes Dev. 2016;30(2):220–32.
Alvarez-Fernandez M, Barrett AJ, Gerhartz B, Dando PM, Ni J, Abrahamson M. Inhibition of mammalian legumain by some cystatins is due to a novel second reactive site. J Biol Chem. 1999;274(27):19195–203.
Andrews NW. Regulated secretion of conventional lysosomes. Trends Cell Biol. 2000;10(8):316–21.
Arampatzidou M, Rehders M, Dauth S, Yu DMT, Tedelind S, Brix K. Imaging of protease functions—current guide to spotting cysteine cathepsins in classical and novel scenes of action in mammalian epithelial cells and tissues. Ital J Anat Embryol. 2011;116(1):1–19.
Arkona C, Wiederanders B. Expression, subcellular distribution and plasma membrane binding of cathepsin B and gelatinases in bone metastatic tissue. Biol Chem. 1996;377(11):695–702.
Baici A, Müntener K, Willimann A, Zwicky R. Regulation of human cathepsin B by alternative mRNA splicing: homeostasis, fatal errors and cell death. Biol Chem. 2006;387(8):1017–21.
Baici A, Novinec M, Lenarčič B. Kinetics of the interaction of peptidases with substrates and modifiers. In: Brix K, Stöcker W, editors. Proteases: structure and function. Berlin: Life Sciences, springer.com; 2013. p. 37–84.
Barrett AJ. The cystatins: a diverse superfamily of cysteine peptidase inhibitors. Biomed Biochim Acta. 1986;45(11–12):1363–74.
Barrett AJ. Bioinformatics of proteases in the MEROPS database. Curr Opin Drug Discov Dev. 2004;7(3):334–41.
Baruch A, Jeffery DA, Bogyo M. Enzyme activity—it’s all about image. Trends Cell Biol. 2004;14(1):29–35.
Bauer S. Toll-like receptor 9 processing: the key event in Toll-like receptor 9 activation? Immunol Lett. 2013;149(1–2):85–7.
Becker BF, Jacob M, Leipert S, Salmon AH, Chappell D. Degradation of the endothelial glycocalyx in clinical settings: searching for the sheddases. Br J Clin Pharmacol. 2015;80(3):389–402.
Blais DR, Nasheri N, McKay CS, Legault MC, Pezacki JP. Activity-based protein profiling of host-virus interactions. Trends Biotechnol. 2012;30(2):89–99.
Blum G. Use of fluorescent imaging to investigate pathological protease activity. Curr Opin Drug Discov Dev. 2008;11(5):708–16.
Blum G, Mullins SR, Keren K, Fonovic M, Jedeszko C, Rice MJ, Sloane BF, Bogyo M. Dynamic imaging of protease activity with fluorescently quenched activity-based probes. Nat Chem Biol. 2005;1(4):203–9.
Blum G, von Degenfeld G, Merchant MJ, Blau HM, Bogyo M. Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes. Nat Chem Biol. 2007;3(10):668–77.
Brix K, Jordans S. Watching proteases in action. Nat Chem Biol. 2005;1(4):186–7.
Brix K, Lemansky P, Herzog V. Evidence for extracellularly acting cathepsins mediating thyroid hormone liberation in thyroid epithelial cells. Endocrinology. 1996;137(5):1963–74.
Brix K, Dunkhorst A, Mayer K, Jordans S. Cysteine cathepsins: cellular roadmap to different functions. Biochimie. 2008;90(2):194–207.
Brix K, Scott CJ, Heck MMS. Compartmentalisation of proteolysis. In: Brix K, Stöcker W, editors. Proteases: structure and function. Berlin: Life Sciences, springer.com; 2013. p. 85–125.
Brix K, McInnes J, Al-Hashimi A, Rehders M, Tamhane T, Haugen MH. Proteolysis mediated by cysteine cathepsins and legumain-recent advances and cell biological challenges. Protoplasma. 2015;252(3):755–74.
Bromme D. Cathepsin F. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004a. p. 1087–8.
Bromme D. Cathepsin K. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004b. p. 1092–7.
Brömme D, Li Z, Barnes M, Mehler E. Human cathepsin V functional expression, tissue distribution, electrostatic surface potential, enzymatic characterization, and chromosomal localization. Biochemistry. 1999;38(8):2377–85.
Brömme D, Panwar P, Turan S. Cathepsin K osteoporosis trials, pycnodysostosis and mouse deficiency models: commonalities and differences. Expert Opin Drug Discovery. 2016;11(5):457–72.
Brzin J, Kopitar M, Turk V, Machleidt W. Protein inhibitors of cysteine proteinases. I. Isolation and characterization of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes. Hoppe Seylers Z Physiol Chem. 1983;364(11):1475–80.
Büth H, Wolters B, Hartwig B, Meier-Bornheim R, Veith H, Hansen M, Sommerhoff CP, Schaschke N, Machleidt W, Fusenig NE, Boukamp P, Brix K. HaCaT keratinocytes secrete lysosomal cysteine proteinases during migration. Eur J Cell Biol. 2004;83(11–12):781–95.
Cavallo-Medved D, Sloane BF. Cell-surface cathepsin B: understanding its functional significance. Curr Top Dev Biol. 2003;54:313–41.
Chen B, Platt MO. Multiplex zymography captures stage-specific activity profiles of cathepsins K, L, and S in human breast, lung, and cervical cancer. J Transl Med. 2011;9:109.
Chen JM, Dando PM, Rawlings ND, Brown MA, Young NE, Stevens RA, Hewitt E, Watts C, Barrett AJ. Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase. J Biol Chem. 1997;272:8090–8.
Cocucci E, Meldolesi J. Ectosomes and exosomes: shedding the confusion between extracellular vesicles. Trends Cell Biol. 2015;25(6):364–72.
Collette J, Bocock JP, Ahn K, Chapman RL, Godbold G, Yeyeodu S, Erickson AH. Biosynthesis and alternate targeting of the lysosomal cysteine protease cathepsin L. Int Rev Cytol. 2004;241:1–51.
Conner GE. Cathepsin D. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 43–52.
Cooper JB. Aspartic proteinases in disease: a structural perspective. Curr Drug Targets. 2002;3(2):155–73.
Cury VF, Gomez RS, Costa JE, Friedman E, Boson W, De Marco L. A homozygous cathepsin C mutation associated with Haim-Munk syndrome. Br J Dermatol. 2005;152:353–6.
Dall E, Brandstetter H. Structure and function of legumain in health and disease. Biochimie. 2016;122:126–50.
Dalton JP, Brindley PJ. Cathepsin W. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. 2004. p. 1109–12.
Danthi P, Guglielmi KM, Kirchner E, Mainou B, Stehle T, Dermody TS. From touchdown to transcription: the reovirus cell entry pathway. Curr Top Microbiol Immunol. 2010;343:91–119.
Davidson Y, Gibbons L, Pritchard A, Hardicre J, Wren J, Tian J, Shi J, Stopford C, Julien C, Thompson J, Payton A, Thaker U, Hayes AJ, Iwatsubo T, Pickering-Brown SM, Pendleton N, Horan MA, Burns A, Purandare N, Lendon CL, Neary D, Snowden JS, Mann DM. Genetic associations between cathepsin D exon 2 C-->T polymorphism and Alzheimer’s disease, and pathological correlations with genotype. J Neurol Neurosurg Psychiatry. 2006;77(4):515–7.
De Duve C, Wattiaux R. Functions of lysosomes. Annu Rev Physiol. 1966;28:435–92.
Dean RT. Lysosomes and protein degradation. Ciba Found Symp. 1979;75:139–49.
Diederich S, Moll M, Klenk HD, Maisner A. The Nipah virus fusion protein is cleaved within the endosomal compartment. J Biol Chem. 2005;280(33):29899–903.
Diederich S, Sauerhering L, Weis M, Altmeppen H, Schaschke N, Reinheckel T, Erbar S, Maisner A. Activation of the Nipah virus fusion protein in MDCK cells is mediated by cathepsin B within the endosome-recycling compartment. J Virol. 2012;86(7):3736–45.
Dolnik O, Stevermann L, Kolesnikova L, Becker S. Marburg virus inclusions: a virus-induced microcompartment and interface to multivesicular bodies and the late endosomal compartment. Eur J Cell Biol. 2015;94(7–9):323–31.
Driessen C, Bryant RA, Lennon-Dumenil AM, Villadangos JA, Bryant PW, Shi GP, Chapman HA, Ploegh HL. Cathepsin S controls the trafficking and maturation of MHC class II molecules in dendritic cells. J Cell Biol. 1999;147:775–90.
Edgington LE, Verdoes M, Bogyo M. Functional imaging of proteases: recent advances in the design and application of substrate-based and activity-based probes. Curr Opin Chem Biol. 2011;15(6):798–805.
Erickson AH, Isidoro C, Mach L, Mort JS. Cathepsins: getting in shape for lysosomal proteolysis. In: Brix K, Stöcker W, editors. Proteases: structure and function. Berlin: Life Sciences, springer.com; 2013. p. 127–73.
Flütsch A, Grütter MG. Proteases in death pathways. In: Brix K, Stöcker W, editors. Proteases: structure and function. Berlin: Life Sciences, springer.com; 2013. p. 265–318.
Fonović M, Turk B. Cysteine cathepsins and extracellular matrix degradation. Biochim Biophys Acta. 2014;1840(8):2560–70.
Friedrichs B, Tepel C, Reinheckel T, Deussing J, von Figura K, Herzog V, Peters C, Saftig P, Brix K. Thyroid functions of mouse cathepsins B, K, and L. J Clin Invest. 2003;111(11):1733–45.
Fritz H. Proteinase inhibitors in severe inflammatory processes (septic shock and experimental endotoxaemia): biochemical, pathophysiological and therapeutic aspects. Ciba Found Symp. 1979;75:351–79.
Frizler M, Yampolsky IV, Baranov MS, Stirnberg M, Gütschow M. Chemical introduction of the green fluorescence: imaging of cysteine cathepsins by an irreversibly locked GFP fluorophore. Org Biomol Chem. 2013;11(35):5913–21.
Gansz M, Kern U, Peters C, Reinheckel T. Exploring systemic functions of lysosomal cysteine proteases: the perspective of genetically modified mouse models. In: Brix K, Stöcker W, editors. Proteases: structure and function. Berlin: Life Sciences, springer.com; 2013. p. 217–33.
Gelb BD, Shi GP, Chapman HA, Desnick RJ. Pycnodysostosis, a lysosomal disease caused by cathepsin K deficiency. Science. 1996;273(5279):1236–8.
Gocheva V, Joyce JA. Cysteine cathepsins and the cutting edge of cancer invasion. Cell Cycle. 2007;6(1):60–4.
Goulet B, Nepveu A. Complete and limited proteolysis in cell cycle progression. Cell Cycle. 2004;3(8):986–9.
Grabowska MM, Day ML. Soluble E-cadherin: more than a symptom of disease. Front Biosci (Landmark Ed). 2012;17:1948–64.
Greenbaum D, Baruch A, Hayrapetian L, Darula Z, Burlingame A, Medzihradszky KF, Bogyo M. Chemical approaches for functionally probing the proteome. Mol Cell Proteomics. 2002;1(1):60–8.
Grzywa R, Sieńczyk M. Phosphonic esters and their application of protease control. Curr Pharm Des. 2013;19(6):1154–78.
Hart TC, Hart PS, Bowden DW, Michalec MD, Callison SA, Walker SJ, Zhang Y, Firatli E. Mutations of the cathepsin C gene are responsible for Papillon-Lefevre syndrome. J Med Genet. 1999;36:881–7.
Hart PS, Zhang Y, Firatli E, Uygur C, Lotfazar M, Michalec MD, Marks JJ, Lu X, Coates BJ, Seow WK, MarshaIl R, Williams D, Reed JB, Wright JT, Hart TC. Identification of cathepsin C mutations in ethnically diverse Papillon-Lefevre syndrome patients. J Med Genet. 2000;37:927–32.
Heald-Sargent T, Gallagher T. Ready, set, fuse! The coronavirus spike protein and acquisition of fusion competence. Virus. 2012;4(4):557–80.
Herz J, Strickland DK. LRP: a multifunctional scavenger and signaling receptor. J Clin Invest. 2001;108(6):779–84.
Hou WS, Bromme D, Zhao Y, Mehler E, Dushey C, Weinstein H, Miranda CS, Fraga C, Greig F, Carey J, Rimoin DL, Desnick RJ, Gelb BD. Characterization of novel cathepsin K mutations in the pro and mature polypeptide regions causing pycnodysostosis. J Clin Invest. 1999;103:731–8.
Hunt CL, Lennemann NJ, Maury W. Filovirus entry: a novelty in the viral fusion world. Virus. 2012;4(2):258–75.
Huntington JA. Shape-shifting serpins--advantages of a mobile mechanism. Trends Biochem Sci. 2006;31(8):427–35.
Hurley JH. ESCRTs are everywhere. EMBO J. 2015;34(19):2398–23407.
Jessani N, Humphrey M, McDonald WH, Niessen S, Masuda K, Gangadharan B, Yates JR 3rd, Mueller BM, Cravatt BF. Carcinoma and stromal enzyme activity profiles associated with breast tumor growth in vivo. Proc Natl Acad Sci U S A. 2004;101(38):13756–61.
Johnson MR, Polymeropoulos MH, Vos HL, Ortiz de Luna RI, Francomano CA. A nonsense mutation in the cathepsin K gene observed in a family with pycnodysostosis. Genome Res. 1996;6:1050–5.
Jordans S, Jenko-Kokalj S, Kuhl NM, Tedelind S, Sendt W, Bromme D, Turk D, Brix K. Monitoring compartment-specific substrate cleavage by cathepsins B, K, L, and S at physiological pH and redox conditions. BMC Biochem. 2009;10:23.
Joyce JA, Hanahan D. Multiple roles for cysteine cathepsins in cancer. Cell Cycle. 2004;3(12):1516–619.
Kay J, Tatnell PJ. Cathepsin E. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 33–8.
Kirschke H. Cathepsin H. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004a. p. 1089–92.
Kirschke H. Cathepsin L. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004b. p. 1097–102.
Kirschke H. Cathepsin S. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004c. p. 1104–7.
Kornfeld S. Structure and function of the mannose 6-phosphate/insulinlike growth factor II receptors. Annu Rev Biochem. 1992;61:307–30.
Kornfeld S, Mellman I. The biogenesis of lysosomes. Annu Rev Cell Biol. 1989;5:483–525.
Krueger S, Kalinski T, Hundertmark T, Wex T, Kuster D, Peitz U, Ebert M, Nagler DK, Kellner U, Malfertheiner P, Naumann M, Rocken C, Roessner A. Up-regulation of cathepsin X in Helicobacter pylori gastritis and gastric cancer. J Pathol. 2005;207:32–42.
Lemansky P, Brix K, Herzog V. Subcellular distribution, secretion, and posttranslational modifications of clusterin in thyrocytes. Exp Cell Res. 1999;251(1):147–55.
Lenarcic B, Turk V. Thyroglobulin type-1 domains in equistatin inhibit both papain-like cysteine proteinases and cathepsin D. J Biol Chem. 1999;274(2):563–6.
Lenarcic B, Ritonja A, Strukelj B, Turk B, Turk V. Equistatin, a new inhibitor of cysteine proteinases from Actinia equina, is structurally related to thyroglobulin type-1 domain. J Biol Chem. 1997;272(21):13899–903.
Li Z, Hou WS, Escalante-Torres CR, Gelb BD, Bromme D. Collagenase activity of cathepsin K depends on complex formation with chondroitin sulfate. J Biol Chem. 2002;277(32):28669–76.
Li Z, Yasuda Y, Li W, Bogyo M, Katz N, Gordon RE, Fields GB, Brömme D. Regulation of collagenase activities of human cathepsins by glycosaminoglycans. J Biol Chem. 2004;279(7):5470–9.
Liaudet-Coopman E, Beaujouin M, Derocq D, Garcia M, Glondu-Lassis M, Laurent-Matha V, Prebois C, Rochefort H, Vignon F. Cathepsin D: newly discovered functions of a long-standing aspartic protease in cancer and apoptosis. Cancer Lett. 2006;237(2):167–79.
Linke M, Herzog V, Brix K. Trafficking of lysosomal cathepsin B-green fluorescent protein to the surface of thyroid epithelial cells involves the endosomal/lysosomal compartment. J Cell Sci. 2002a;115(Pt 24):4877–89.
Linke M, Jordans S, Mach L, Herzog V, Brix K. Thyroid stimulating hormone upregulates secretion of cathepsin B from thyroid epithelial cells. Biol Chem. 2002b;383(5):773–84.
Luke CJ, Pak SC, Askew YS, Naviglia TL, Askew DJ, Nobar SM, Vetica AC, Long OS, Watkins SC, Stolz DB, Barstead RJ, Moulder GL, Brömme D, Silverman GA. An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury. Cell. 2007;130(6):1108–19.
Machleidt W, Borchart U, Fritz H, Brzin J, Ritonja A, Turk V. Protein inhibitors of cysteine proteinases. II. Primary structure of stefin, a cytosolic protein inhibitor of cysteine proteinases from human polymorphonuclear granulocytes. Hoppe Seylers Z Physiol Chem. 1983;364(11):1481–6.
Mason RW, Gal S, Gottesman MM. The identification of the major excreted protein (MEP) from a transformed mouse fibroblast cell line as a catalytically active precursor form of cathepsin L. Biochem J. 1987;248(2):449–54.
Mayer K, Iolyeva ME, Meyer-Grahle U, Brix K. Intestine-specific expression of green fluorescent protein-tagged cathepsin B: proof-of-principle experiments. Biol Chem. 2008;389(8):1085–96.
Mehtani S, Gong Q, Panella J, Subbiah S, Peffley DM, Frankfater A. In vivo expression of an alternatively spliced human tumor message that encodes a truncated form of cathepsin B. Subcellular distribution of the truncated enzyme in COS cells. J Biol Chem. 1998;273(21):13236–44.
Menard R, Sulea T. Cathepsin X. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 1113–6.
Meulendyke KA, Wurth MA, McCann RO, Dutch RE. Endocytosis plays a critical role in proteolytic processing of the Hendra virus fusion protein. J Virol. 2005;79(20):12643–9.
Millet JK, Whittaker GR. Host cell proteases: critical determinants of coronavirus tropism and pathogenesis. Virus Res. 2015;202:120–34.
Mohamed MM, Sloane BF. Cysteine cathepsins: multifunctional enzymes in cancer. Nat Rev Cancer. 2006;6(10):764–75.
Mort JS. Cathepsin B. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 1079–86.
Mort JS, Buttle DJ. Cathepsin B. Int J Biochem Cell Biol. 1997;29(5):715–20.
Moyle G, Gazzard B. Current knowledge and future prospects for the use of HIV protease inhibitors. Drugs. 1996;51(5):701–12.
Müntener K, Zwicky R, Csucs G, Rohrer J, Baici A. Exon skipping of cathepsin B: mitochondrial targeting of a lysosomal peptidase provokes cell death. J Biol Chem. 2004;279(39):41012–7.
Naganawa Y, Itoh K, Shimmoto M, Kamei S, Takiguchi K, Doi H, Sakuraba H. Stable expression of protective protein/cathepsin A-green fluorescent protein fusion genes in a fibroblastic cell line from a galactosialidosis patient. Model system for revealing the intracellular transport of normal and mutated lysosomal enzymes. Biochem J. 1999;340(Pt 2):467–74.
Nielsen R, Courtoy PJ, Jacobsen C, Dom G, Lima WR, Jadot M, Willnow TE, Devuyst O, Christensen EI. Endocytosis provides a major alternative pathway for lysosomal biogenesis in kidney proximal tubular cells. Proc Natl Acad Sci U S A. 2007;104(13):5407–12.
Novinec M, Lenarčič B, Baici A. Clusterin is a specific stabilizer and liberator of extracellular cathepsin K. FEBS Lett. 2012;586(7):1062–6.
Obermajer N, Magister S, Kopitar AN, Tepes B, Ihan A, Kos J. Cathepsin X prevents an effective immune response against Helicobacter pylori infection. Eur J Cell Biol. 2009;88:461–71.
Ong PC, McGowan S, Pearce MC, Irving JA, Kan WT, Grigoryev SA, Turk B, Silverman GA, Brix K, Bottomley SP, Whisstock JC, Pike RN. DNA accelerates the inhibition of human cathepsin V by serpins. J Biol Chem. 2007;282(51):36980–6.
Pager CT, Dutch RE. Cathepsin L is involved in proteolytic processing of the Hendra virus fusion protein. J Virol. 2005;79(20):12714–20.
Pager CT, Craft WWJ, Patch J, Dutch RE. A mature and fusogenic form of the Nipah virus fusion protein requires proteolytic processing by cathepsin L. Virology. 2006;346(2):251–7.
Peloille S, Esnard A, Dacheux JL, Guillou F, Gauthier F, Esnard F. Interactions between ovine cathepsin L, cystatin C and alpha 2-macroglobulin. Potential role in the genital tract. Eur J Biochem. 1997;244(1):140–6.
Platt MO, Evans D, Keegan PM, McNamara L, Parker IK, Roberts LM, Caulk AW, Gleason RLJ, Seifu D, Amogne W, Penny C. Low-cost method to monitor patient adherence to HIV antiretroviral therapy using multiplex Cathepsin zymography. Mol Biotechnol. 2016;58(1):56–64.
Pohlmann R, Boeker MW, von Figura K. The two mannose 6-phosphate receptors transport distinct complements of lysosomal proteins. J Biol Chem. 1995;270(45):27311–8.
Poller W, Willnow TE, Hilpert J, Herz J. Differential recognition of alpha 1-antitrypsin-elastase and alpha 1-antichymotrypsin-cathepsin G complexes by the low density lipoprotein receptor-related protein. J Biol Chem. 1995;270(6):2841–5.
Pshezhetsky AV. Lysosomal carboxypeptidase A. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 1923–9.
Rachner TD, Khosla S, Hofbauer LC. Osteoporosis: now and the future. Lancet. 2011;377(9773):1276–87.
Rawlings ND. Protease families, evolution and mechanism of action. In: Brix K, Stöcker W, editors. Proteases: structure and function. Berlin: Life Sciences, springer.com; 2013. p. 1–36.
Rawlings ND, Barrett AJ, Finn R. Twenty years of the MEROPS database of proteolytic enzymes, their substrates and inhibitors. Nucleic Acids Res. 2016;44(D1):D343–50.
Rawn SM, Cross JC. The evolution, regulation, and function of placenta-specific genes. Annu Rev Cell Dev Biol. 2008;24:159–81.
Reiser J, Adair B, Reinheckel T. Specialized roles for cysteine cathepsins in health and disease. J Clin Invest. 2010;120(10):3421–31.
Rivera LE, Colon K, Cantres-Rosario YM, Zenon FM, Melendez LM. Macrophage derived cystatin B/cathepsin B in HIV replication and neuropathogenesis. Curr HIV Res. 2014;12(2):111–20.
Rochefort H, Garcia M, Glondu M, Laurent V, Liaudet E, Rey JM, Roger P. Cathepsin D in breast cancer: mechanisms and clinical applications, a 1999 overview. Clin Chim Acta. 2000;291(2):157–70.
Rock KL, Shen L. Cross-presentation: underlying mechanisms and role in immune surveillance. Immunol Rev. 2005;207:166–83.
Rubin H. Systemic effects of cancer: role of multiple proteases and their toxic peptide products. Med Sci Monit. 2005;11(7):RA221–8.
Sadaghiani AM, Verhelst SH, Gocheva V, Hill K, Majerova E, Stinson S, Joyce JA, Bogyo M. Design, synthesis, and evaluation of in vivo potency and selectivity of epoxysuccinyl-based inhibitors of papain-family cysteine proteases. Chem Biol. 2007;14(5):499–511.
Saftig P, Hunziker E, Wehmeyer O, Jones S, Boyde A, Rommerskirch W, Moritz JD, Schu P, von Figura K. Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-K-deficient mice. Proc Natl Acad Sci U S A. 1998;95(23):13453–8.
Salpeter SJ, Blum G. Ready, set, cleave: proteases in action. Chem Biol. 2013;20(2):137–8.
Salvesen GS. Cathepsin G. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 1524–6.
Sanman LE, Bogyo M. Activity-based profiling of proteases. Annu Rev Biochem. 2014;83:249–73.
Scharf JG, Braulke T. The role of the IGF axis in hepatocarcinogenesis. Horm Metab Res. 2003;35(11–12):685–93.
Schaschke N, Assfalg-Machleidt I, Machleidt W, Moroder L. Substrate/propeptide-derived endo-epoxysuccinyl peptides as highly potent and selective cathepsin B inhibitors. FEBS Lett. 1998;421(1):80–2.
Schechter I, Berger A. On the active site of proteases. 3. Mapping the active site of papain; specific peptide inhibitors of papain. Biochem Biophys Res Commun. 1968;32(5):898–902.
Schilling K, Körner A, Sehmisch S, Kreusch A, Kleint R, Benedix Y, Schlabrakowski A, Wiederanders B. Selectivity of propeptide-enzyme interaction in cathepsin L-like cysteine proteases. Biol Chem. 2009;390(2):167–74.
Siintola E, Partanen S, Strömme P, Haapanen A, Haltia M, Maehlen J, Lehesjoki AE, Tyynelä J. Cathepsin D deficiency underlies congenital human neuronal ceroid-lipofuscinosis. Brain. 2006;129(Pt 6):1438–45.
Silverman GA, Bird PI, Carrell RW, Church FC, Coughlin PB, Gettins PG, Irving JA, Lomas DA, Luke CJ, Moyer RW, Pemberton PA, Remold-O'Donnell E, Salvesen GS, Travis J, Whisstock JC. The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. Evolution, mechanism of inhibition, novel functions, and a revised nomenclature. J Biol Chem. 2001;276(36):33293–6.
Simmons G, Zmora P, Gierer S, Heurich A, Pöhlmann S. Proteolytic activation of the SARS-coronavirus spike protein: cutting enzymes at the cutting edge of antiviral research. Antiviral Res. 2013;100(3):605–14.
Sloane BF, Moin K, Krepela E, Rozhin J. Cathepsin B and its endogenous inhibitors: the role in tumor malignancy. Cancer Metastasis Rev. 1990;9(4):333–52.
Sloane BF, List K, Fingleton B, Matrisian L. Proteases in cancer—significance for invasion and metastasis. In: Brix K, Stöcker W, editors. Proteases: structure and function. Berlin: Life Sciences, springer.com; 2013. p. TBD.
Spiess E, Brüning A, Gack S, Ulbricht B, Spring H, Trefz G, Ebert W. Cathepsin B activity in human lung tumor cell lines: ultrastructural localization, pH sensitivity, and inhibitor status at the cellular level. J Histochem Cytochem. 1994;42(7):917–29.
Sun L, Liu S, Chen ZJ. SnapShot: pathways of antiviral innate immunity. Cell. 2010;140:436. https://doi.org/10.1016/j.cell.2010.01.041.
Tamhane T, Wolters BK, Illukkumbura R, Maelandsmo GM, Haugen MH, Brix K. Construction of a plasmid coding for green fluorescent protein tagged cathepsin L and data on expression in colorectal carcinoma cells. Data Brief. 2015;5:468–75.
Tamhane T, Lllukkumbura R, Lu S, Maelandsmo GM, Haugen MH, Brix K. Nuclear cathepsin L activity is required for cell cycle progression of colorectal carcinoma cells. Biochimie. 2016;122:208–18.
Tedelind S, Poliakova K, Valeta A, Hunegnaw R, Yemanaberhan EL, Heldin NE, Kurebayashi J, Weber E, Kopitar-Jerala N, Turk B, Bogyo M, Brix K. Nuclear cysteine cathepsin variants in thyroid carcinoma cells. Biol Chem. 2010;391(8):923–35.
Tepel C, Bromme D, Herzog V, Brix K. Cathepsin K in thyroid epithelial cells: sequence, localization and possible function in extracellular proteolysis of thyroglobulin. J Cell Sci. 2000;113(Pt 24):4487–98.
Tholen M, Hillebrand LE, Tholen S, Sedelmeier O, Arnold SJ, Reinheckel T. Out-of-frame start codons prevent translation of truncated nucleo-cytosolic cathepsin L in vivo. Nat Commun. 2014;5:4931.
Tolosa E, Li W, Yasuda Y, Wienhold W, Denzin LK, Lautwein A, Driessen C, Schnorrer P, Weber E, Stevanovic S, Kurek R, Melms A, Bromme D. Cathepsin V is involved in the degradation of invariant chain in human thymus and is overexpressed in myasthenia gravis. J Clin Invest. 2003;112:517–26.
Tong TR. SARS coronavirus anti-infectives. Recent Pat Antiinfect Drug Discov. 2006;1(3):297–308.
Toomes C, James J, Wood AJ, Wu CL, McCormick D, Lench N, Hewitt C, Moynihan L, Roberts E, Woods CG, Markham A, Wong M, Widmer R, Ghaffar KA, Pemberton M, Hussein IR, Temtamy SA, Davies R, Read AP, Sloan P, Dixon MJ, Thakker NS. Loss-of-function mutations in the cathepsin C gene result in periodontal disease and palmoplantar keratosis. Nat Genet. 1999;23:421–4.
Travis J. Structure, function, and control of neutrophil proteinases. Am J Med. 1988;84(6A):37–42.
Turk V, Bode W. The cystatins: protein inhibitors of cysteine proteinases. FEBS Lett. 1991;285(2):213–9.
Turk B, Turk V. Lysosomes as “suicide bags” in cell death: myth or reality? J Biol Chem. 2009;284(33):21783–7.
Turk B, Turk D, Turk V. Lysosomal cysteine proteases: more than scavengers. Biochim Biophys Acta. 2000;1477(1–2):98–111.
Turk B, Turk D, Dolenc I, Turk V. Dipeptidyl-peptidase I. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 1192–6.
Turk V, Stoka V, Turk D. Cystatins: biochemical and structural properties, and medical relevance. Front Biosci. 2008;(13):5406–20.
Velasco G, Lopez-Otin C. Cathepsin O. In: Barrett AJ, Rawlings ND, Woessner JF, editors. Handbook of proteolytic enzymes. 2nd ed. London: Elsevier; 2004. p. 1102–3.
Vogt C, Eickmann M, Diederich S, Moll M, Maisner A. Endocytosis of the Nipah virus glycoproteins. J Virol. 2005;79(6):3865–72.
von Figura K. Molecular recognition and targeting of lysosomal proteins. Curr Opin Cell Biol. 1991;3(4):642–6.
Weber E, Barbulescu E, Medek R, Reinheckel T, Sameni M, Anbalagan A, Moin K, Sloane BF. Cathepsin B-deficient mice as source of monoclonal anti-cathepsin B antibodies. Biol Chem. 2015;396(3):277–81.
Weis M, Maisner A. Nipah virus fusion protein: importance of the cytoplasmic tail for endosomal trafficking and bioactivity. Eur J Cell Biol. 2015;94(7–9):316–22.
Weiss-Sadan T, Gotsman I, Blum G. Cysteine proteases in atherosclerosis. FEBS J. 2017. https://doi.org/10.1111/febs.14043. (Epub ahead of print).
Whisstock JC, Silverman GA, Bird PI, Bottomley SP, Kaiserman D, Luke CJ, Pak SC, Reichhart JM, Huntington JA. Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions. J Biol Chem. 2010;285(32):24307–12.
Willnow TE, Moehring JM, Inocencio NM, Moehring TJ, Herz J. The low-density-lipoprotein receptor-related protein (LRP) is processed by furin in vivo and in vitro. Biochem J. 1996;313(Pt 1):71–6.
Zeeuwen PL, Cheng T, Schalkwijk J. The biology of cystatin M/E and its cognate target proteases. J Invest Dermatol. 2009;129(6):1327–38.
Zhou XY, Morreau H, Rottier R, Davis D, Bonten E, Gillemans N, Wenger D, Grosveld FG, Doherty P, Suzuki K, Grosveld GC, D'Azzo A. Mouse model for the lysosomal disorder galactosialidosis and correction of the phenotype with overexpressing erythroid precursor cells. Genes Dev. 1995;9:2623–34.
Zou F, Schmon M, Sienczyk M, Grzywa R, Palesch D, Boehm BO, Sun ZL, Watts C, Schirmbeck R, Burster T. Application of a novel highly sensitive activity-based probe for detection of cathepsin G. Anal Biochem. 2012;421(2):667–72.
Zwicky R, Müntener K, Csucs G, Goldring MB, Baici A. Exploring the role of 5′ alternative splicing and of the 3′-untranslated region of cathepsin B mRNA. Biol Chem. 2003;384(7):1007–18.
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Brix, K. (2018). Host Cell Proteases: Cathepsins. In: Böttcher-Friebertshäuser, E., Garten, W., Klenk, H. (eds) Activation of Viruses by Host Proteases. Springer, Cham. https://doi.org/10.1007/978-3-319-75474-1_10
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