Abstract
In humans cone photoreceptors are responsible for high-resolution colour vision. A variety of retinal diseases can compromise cone viability, and, at present, no satisfactory treatment options are available. Here, we present data towards establishing a reliable, high-throughput assay system that will facilitate the search for cone neuroprotective compounds using the murine-photoreceptor cell line 661 W. To further characterize 661 W cells, a retinal marker study was performed, followed by the induction of cell death using paradigms over-activating cGMP-dependent protein kinase G (PKG). We found that 661 W cells may be used to mimic specific aspects of cone degeneration and may thus be valuable for future compound screening studies.
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Acknowledgments and Funding
We thank Bernd Wissinger and Peggy Reuter for helpful discussions and Sandra Bernhard-Kurz and Norman Rieger for skilful technical assistance. This work was supported by grants from the Tistou and Charlotte Kerstan Foundation, the European Union (DRUGSFORD; HEALTH-F2-2012-304963), and Deutsche Forschungsgemeinschaft (PA1751/1-1).
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Mencl, S., Trifunović, D., Zrenner, E., Paquet-Durand, F. (2018). PKG-Dependent Cell Death in 661W Cone Photoreceptor-like Cell Cultures (Experimental Study). In: Ash, J., Anderson, R., LaVail, M., Bowes Rickman, C., Hollyfield, J., Grimm, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 1074. Springer, Cham. https://doi.org/10.1007/978-3-319-75402-4_63
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DOI: https://doi.org/10.1007/978-3-319-75402-4_63
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