Normative Retinal Thicknesses in Common Animal Models of Eye Disease Using Spectral Domain Optical Coherence Tomography

Carpenter, Christy L.; Kim, Alice Y.; Kashani, Amir H.

doi:10.1007/978-3-319-75402-4_20

Christy L. Carpenter¹²,
Alice Y. Kim¹² &
Amir H. Kashani^12,13

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1074))

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Abstract

Purpose

This study demonstrates a standardized approach to measuring retinal thickness (RT) using spectral domain optical coherence tomography (SD-OCT) in commonly used animal models of disease and reports a normative data set for future use.

Materials and Methods

Twenty normal eyes of 4 adult animal models (5 rats, 5 rabbits, 5 canines, and 5 mini-pigs) were used. Manual measurements were made on the commercially available Heidelberg Spectralis™ SD-OCT to determine the total, inner, and outer retinal thickness (RT) at fixed distances from the optic nerve head (ONH) (1, 2, 3, 4, 5, and 6 mm away) in order to control for normal variation in retinal thickness. Analysis of variance (ANOVA) with P value <0.05 indicated statistical significance.

Results

Total RT significantly decreased with increasing distance from the ONH for the canine, mini-pig, and rabbit vascular models. Inner RT significantly decreased for the canine, mini-pig, rabbit vascular, and rabbit avascular models; and outer RT significantly decreased for only the canine model. Among the animal models, RT at similar distances from the ONH were significantly different for total, inner, and outer RT.

Conclusion

There are significant differences in the total, inner, and outer RT of normal canine, mini-pig, rabbit, and rat retinas with SD-OCT using a standardized approach. These measurements provide a normative reference for future studies and illustrate a standardized method of assessing RT.

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Authors and Affiliations

Department of Ophthalmology, USC Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, California, USA
Christy L. Carpenter, Alice Y. Kim & Amir H. Kashani
USC Roski Eye Institute, Los Angeles, CA, USA
Amir H. Kashani

Authors

Christy L. Carpenter
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Alice Y. Kim
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Amir H. Kashani
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Corresponding author

Correspondence to Amir H. Kashani .

Editor information

Editors and Affiliations

Department of Ophthalmology, University of Florida, Gainesville, Florida, USA
John D. Ash
Ophthalmology and Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
Robert E. Anderson
School of Medicine Beckman Vision Center, University of California San Francisco, San Francisco, California, USA
Matthew M. LaVail
Departments of Ophthalmology and Cell Biology, Duke Eye Center, Duke University Medical Center, Durham, North Carolina, USA
Catherine Bowes Rickman
Division of Ophthalmology, Case Western Reserve University, Cleveland, Ohio, USA
Joe G. Hollyfield
Department of Ophthalmology, University Hospital Zurich, Zurich, Switzerland
Christian Grimm

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Carpenter, C.L., Kim, A.Y., Kashani, A.H. (2018). Normative Retinal Thicknesses in Common Animal Models of Eye Disease Using Spectral Domain Optical Coherence Tomography. In: Ash, J., Anderson, R., LaVail, M., Bowes Rickman, C., Hollyfield, J., Grimm, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 1074. Springer, Cham. https://doi.org/10.1007/978-3-319-75402-4_20

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DOI: https://doi.org/10.1007/978-3-319-75402-4_20
Published: 03 May 2018
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-75401-7
Online ISBN: 978-3-319-75402-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)

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