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Confocal Raman Microscopy pp 471–482Cite as

Raman Imaging of Plant Cell Walls

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Part of the book series: Springer Series in Surface Sciences ((SSSUR,volume 66))

Abstract

To gain a better understanding of plant cell walls, several microscopic and chemical methods have been used for their analysis in recent years. However, a knowledge gap exists about the location, quantity and structural arrangement of molecules on the micrometer scale within the native cell wall. Advances in confocal Raman microscopy and imaging have tackled this problem in a non-invasive way and provide chemical and structural information in-situ with a high spatial resolution (<0.5 \(\upmu {\mathrm m}\)). Examples of high resolution Raman imaging on wood cells are given, showing that changes in polymer chemistry and orientation can be followed within and between different cell wall layers. In horsetail (Equisetum hyemale) tissue, in addition to the mapping of plant cell wall polymers, the distribution of amorphous silica is investigated. Area scans from a cross section are included as well as a depth profiling within a silica-rich knob on the outer stem wall.

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Acknowledgements

R. Nöske (Potsdam University, Department of Chemistry) is thanked for providing the horsetail sample, B. Clair (Universite Montpellier) for the tropical wood sample and I. Burgert, O. Paris and P. Fratzl (Max Planck Institute of Colloids and Interfaces, Department Biomaterials, Potsdam) for enabling the work. The author acknowledges the APART program of the Austrian Academy of Sciences and the Max Planck Society for funding.

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Authors and Affiliations

  1. Department of Nanobiotechnology, Universität für Bodenkultur Wien, Muthgasse 11/II 69, 1190, Vienna, Austria

    Notburga Gierlinger

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  1. Notburga Gierlinger
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Correspondence to Notburga Gierlinger .

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Editors and Affiliations

  1. Wissenschaftliche Instrumente und Technologie GmbH, Ulm, Germany

    Jan Toporski

  2. Ulm, Germany

    Thomas Dieing

  3. Wissenschaftliche Instrumente und Technologie GmbH, Ulm, Germany

    Olaf Hollricher

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Gierlinger, N. (2018). Raman Imaging of Plant Cell Walls. In: Toporski, J., Dieing, T., Hollricher, O. (eds) Confocal Raman Microscopy. Springer Series in Surface Sciences, vol 66. Springer, Cham. https://doi.org/10.1007/978-3-319-75380-5_19

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