Abstract
This chapter provides an overview of value-added production using extremophiles as well as the advantages and challenges for process development in a biorefinery concept. The chapter then shows a modeling framework that includes metabolic flux modeling, growth kinetics, and bioreactor models as well as process simulation. The model results are the basis for optimization, economic analysis, and life cycle assessment. The tools are applied to the production of poly-3-hydroxybutyrate (PHB) by using the halophilic bacteria Halomonas sp. The results highlighted the importance of relating models at the various scales and to look at the whole process picture to optimize the economic and environmental performances of the resulting biorefinery process. In the optimized process, the minimum PHB selling price was $7.05 per kg and the reduction in greenhouse gas emissions was 90%, with 0.708 kg CO-eq per kg of PHB. These results showed the potential for using halophilic bacteria to make PHB production competitive in terms of economics and environmental impacts. This also shows how extremophile processing will play a key role in making biorefineries more profitable and sustainable.
Keywords
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- ATP:
-
Adenosine triphosphate
- CHP:
-
Combined heat and power
- CSTR:
-
Continuously stirred tank reactor
- GHG:
-
Greenhouse gas
- GWP:
-
Global warming potential
- LCA:
-
Life cycle assessment
- MFA:
-
Metabolic flux analysis
- NADH:
-
Nicotinamide adenine dinucleotide
- NADPH:
-
Nicotinamide adenosine dinucleotide phosphate
- PHB:
-
Poly-3-hydroxybutyrate
- PLA:
-
Polylactic acid
- SDS:
-
Sodium dodecyl sulfonate
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Financial support provided by the National Science Foundation in the form of BuG ReMeDEE initiative (Award # 1736255) is gratefully acknowledged by the editors.
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Martinez-Hernandez, E., Ng, K.S., Amezcua Allieri, M.A., Aburto Anell, J.A., Sadhukhan, J. (2018). Value-Added Products from Wastes Using Extremophiles in Biorefineries: Process Modeling, Simulation, and Optimization Tools. In: Sani, R., Krishnaraj Rathinam, N. (eds) Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power. Springer, Cham. https://doi.org/10.1007/978-3-319-74459-9_14
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DOI: https://doi.org/10.1007/978-3-319-74459-9_14
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