Abstract
As outlined in Chap. 6, cultured cells have become an indispensable technology in various branches of life sciences. However, there are some concerns associated with the study of cultured cells.
Parts of the text and figures of this chapter are reprinted with permission from Antioxidants and Redox Signalling, Volume 24, Issue 13, published by Mary Ann Leibert, Inc., New Rochelle, NY., and Organic and Biomolecular Chemistry, Issue 24, with permission from the Royal Society of Chemistry.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
G. Kaur, J.M. Dufour, Cell lines. Spermatogenesis 2, 1–5 (2012)
J.R. Masters, G.N. Stacey, Changing medium and passaging cell lines. Nat. Protocols 2, 2276–2284 (2007)
M. Lacroix, Persistent use of false cell lines. Int. J. Cancer 122, 1–4 (2008)
J.-P. Gillet, S. Varma, M.M. Gottesman, The clinical relevance of cancer cell lines. J. Natl. Cancer Inst. 105, 452–458 (2013)
I. Garitaonandia, H. Amir, F.S. Boscolo, G.K. Wambua, H.L. Schultheisz, K. Sabatini, R. Morey, S. Waltz, Y.-C. Wang, H. Tran, T.R. Leonardo, K. Nazor, I. Slavin, C. Lynch, Y. Li, R. Coleman, I. Gallego Romero, G. Altun, D. Reynolds, S. Dalton, M. Parast, J.F. Loring, L.C. Laurent, Increased risk of genetic and epigenetic instability in human embryonic stem cells associated with specific culture conditions. PLoS ONE 10, e0118307 (2015)
C. Pan, C. Kumar, S. Bohl, U. Klingmueller, M. Mann, Comparative proteomic phenotyping of cell lines and primary cells to assess preservation of cell type-specific functions. Mol. Cellular Proteomics 8, 443–450 (2009)
S. Wilkening, F. Stahl, A. Bader, comparison of primary human hepatocytes and hepatoma cell line HEPG2 with regard to their biotransformation properties. Drug Metab. Dispos. 31, 1035–1042 (2003)
V. Sanchez-Valle, N.C. Chavez-Tapia, M. Uribe, N. Mendez-Sanchez, Role of oxidative stress and molecular changes in liver fibrosis: a review. Curr. Med. Chem. 19, 4850–4860 (2012)
C. Guguen-Guillouzo, A. Guillouzo, General review on in vitro hepatocyte models and their applications, in Methods in Molecular Biology, ed. by N.J. Clifton, vol. 640 (2010), pp. 1–40
C. Garcia-Ruiz, J.C. Fernandez-Checa, Redox regulation of hepatocyte apoptosis. J. Gastroenterol. Hepatol. 22(Suppl 1), S38–42 (2007)
R. Singh, M.J. Czaja, Regulation of hepatocyte apoptosis by oxidative stress. J. Gastroenterol. Hepatol. 22(Suppl 1), S45–8 (2007)
I. Kurose, H. Higuchi, S. Miura, H. Saito, N. Watanabe, R. Hokari, M. Hirokawa, M. Takaishi, S. Zeki, T. Nakamura, H. Ebinuma, S. Kato, H. Ishii, Oxidative stress-mediated apoptosis of hepatocytes exposed to acute ethanol intoxication. Hepatology 25, 368–378 (1997)
Y. Sumida, E. Niki, Y. Naito, T. Yoshikawa, Involvement of free radicals and oxidative stress in NAFLD/NASH. Free Radic. Res. 47, 869–880 (2013)
M.D. Norenberg, A.R. Jayakumar, K.V. Rama, Rao, oxidative stress in the pathogenesis of hepatic encephalopathy. Metab. Brain Dis. 19, 313–329 (2004)
H. Tsukamoto, Oxidative stress, antioxidants, and alcoholic liver fibrogenesis, in Alcohol (Fayetteville, N.Y.), vol. 10 (1993), pp. 465–467
S. Pal, S.J. Polyak, N. Bano, W.C. Qiu, R.L. Carithers, M. Shuhart, D.R. Gretch, A. Das, Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1. J. Gastroenterol. Hepatol. 25, 627–634 (2010)
H. Cichoż-Lach, A. Michalak, Oxidative stress as a crucial factor in liver diseases. World J. Gastroenterol. WJG 20, 8082–8091 (2014)
B. Saberi, M. Shinohara, M.D. Ybanez, N. Hanawa, W.A. Gaarde, N. Kaplowitz, D. Han, Regulation of H(2)O(2)-induced necrosis by PKC and AMP-activated kinase signaling in primary cultured hepatocytes. Am. J. Physiol. Cell Physiol. 295, C50–63 (2008)
M.G. Cotticelli, A.M. Crabbe, R.B. Wilson, M.S. Shchepinov, Insights into the role of oxidative stress in the pathology of Friedreich ataxia using peroxidation resistant polyunsaturated fatty acids. Redox Biol. 1, 398–404 (2013)
S. Hill, C.R. Lamberson, L. Xu, R. To, H.S. Tsui, V.V. Shmanai, A.V. Bekish, A.M. Awad, B.N. Marbois, C.R. Cantor, N.A. Porter, C.F. Clarke, M.S. Shchepinov, Small amounts of isotope-reinforced polyunsaturated fatty acids suppress lipid autoxidation. Free Radic. Biol. Med. 53, 893–906 (2012)
L.A. Herzenberg, D. Parks, B. Sahaf, O. Perez, M. Roederer, L.A. Herzenberg, The history and future of the fluorescence activated cell sorter and flow cytometry: a view from stanford. Clin. Chem. 48, 1819–1827 (2002)
Regenerative Medicine, Technical report, Department of Health and Human Services
D. Levitt, R. Mertelsmann, Hematopoietic Stem Cells: Biology and Therapeutic Applications (Taylor & Francis, 1995)
C.J. Eaves, Hematopoietic stem cells: concepts, definitions, and the new reality. Blood 125, 2605–2613 (2015)
I. Godin, A. Cumano, Hematopoietic Stem Cell Development (Medical Intelligence Unit, Springer, US, 2010)
M. Kondo, Hematopoietic Stem Cell Biology. Stem Cell Biology and Regenerative Medicine (Humana Press, 2009)
D. Hernandez-Garcia, C.D. Wood, S. Castro-Obregon, L. Covarrubias, Reactive oxygen species: a radical role in development? Free Radic. Biol. Med. 49, 130–143 (2010)
H. Sandoval, P. Thiagarajan, S.K. Dasgupta, A. Schumacher, J.T. Prchal, M. Chen, J. Wang, Essential role for Nix in autophagic maturation of erythroid cells. Nature 454, 232–235 (2008)
N.A. Maianski, J. Geissler, S.M. Srinivasula, E.S. Alnemri, D. Roos, T.W. Kuijpers, Functional characterization of mitochondria in neutrophils: a role restricted to apoptosis. Cell Death Differ. 11, 143–153 (2004)
C. Nombela-Arrieta, G. Pivarnik, B. Winkel, K.J. Canty, B. Harley, J.E. Mahoney, S.-Y. Park, J. Lu, A. Protopopov, L.E. Silberstein, Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment. Nat. Cell Biol. 15, 533–543 (2013)
H.M. Shapiro, Practical Flow Cytometry (Wiley, 2005)
S.T. Fraser, R.G. Midwinter, B.S. Berger, R. Stocker, Heme oxygenase-1: a critical link between iron metabolism, erythropoiesis, and development. Adv. Hematol. 2011, 473709 (2011)
J. Isern, S.T. Fraser, Z. He, M.H. Baron, Developmental niches for embryonic erythroid cells. Blood Cells Molecules Dis. 44, 207–208 (2010)
K. McGrath, J. Palis, Ontogeny of erythropoiesis in the mammalian embryo. Curr. Top. Dev. Biol. 82, 1–22 (2008)
P.D. Kingsley, J. Malik, K.A. Fantauzzo, J. Palis, Yolk sac-derived primitive erythroblasts enucleate during mammalian embryogenesis. Blood 104, 19–25 (2004)
S.T. Fraser, J. Isern, M.H. Baron, Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression. Blood 109, 343–352 (2006)
M. Socolovsky, Molecular insights into stress erythropoiesis. Curr. Opin. Hematol. 14, 215–224 (2007)
M.H. Baron, Embryonic origins of mammalian hematopoiesis. Exp. Hematol. 31, 1160–1169 (2003)
K. Ito, A. Hirao, F. Arai, S. Matsuoka, K. Takubo, I. Hamaguchi, K. Nomiyama, K. Hosokawa, K. Sakurada, N. Nakagata, Y. Ikeda, T.W. Mak, T. Suda, Regulation of oxidative stress by ATM is required for self-renewal of haematopoietic stem cells. Nature 431, 997–1002 (2004)
Y.-Y. Jang, S.J. Sharkis, A low level of reactive oxygen species selects for primitive hematopoietic stem cells that may reside in the low-oxygenic niche. Blood 110, 3056–3063 (2007)
P. Rimmelé, C. Bigarella, R. Liang, B. Izac, R. Dieguez-Gonzalez, G. Barbet, M. Donovan, C. Brugnara, J. Blander, D. Sinclair, S. Ghaffari, Aging-like phenotype and defective lineage specification in SIRT1-deleted hematopoietic stem and progenitor cells. Stem Cell Rep. 3, 44–59 (2016)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG
About this chapter
Cite this chapter
Kaur, A. (2018). Ex Vivo Studies. In: Fluorescent Tools for Imaging Oxidative Stress in Biology. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-73405-7_7
Download citation
DOI: https://doi.org/10.1007/978-3-319-73405-7_7
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-73404-0
Online ISBN: 978-3-319-73405-7
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)