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Abstract

\(^{1}\)H NMR spectra were obtained at 300 K using Bruker DRX 300 or Bruker Ascend 400 and 500 spectrometers at frequencies of 300, 400 and 500 MHz, respectively. \(^{13}\)C NMR spectra were recorded on these spectrometers at frequencies of 75, 100, and 125 MHz. Deuterated solvents were obtained from Cambridge Isotope Laboratories were used, and internal references were made using the residual solvent peak. The \(^{1}\)H NMR data are reported as chemical shift (\(\delta \)) in ppm, and coupling constant (J) in Hz. The \(^{13}\)C NMR data are reported as chemical shift (\(\delta \)) in ppm downfield shift.

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References

  1. T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55–63 (1983)

    Article  CAS  Google Scholar 

  2. V.I. Antas, K.W. Brigden, A.J. Prudence, S.T. Fraser, Gastrokine-2 is transiently expressed in the endodermal and endothelial cells of the maturing mouse yolk sac. Gene Expr. Patterns 16, 69–74 (2014)

    Article  CAS  Google Scholar 

  3. S.T. Fraser, M. Ogawa, T. Yokomizo, Y. Ito, S. Nishikawa, S.-I. Nishikawa, Putative intermediate precursor between hematogenic endothelial cells and blood cells in the developing embryo. Dev. Growth Differ. 45, 63–75 (2003)

    Article  CAS  Google Scholar 

  4. J. Isern, S.T. Fraser, Z. He, M.H. Baron, The fetal liver is a niche for maturation of primitive erythroid cells. Proc. Natl. Acad. Sci. U S A 105, 6662–6667 (2008)

    Google Scholar 

  5. Y.-Y. Zhang, J.-L. Mi, C.-H. Zhou, X.-D. Zhou, Synthesis of novel fluconazoliums and their evaluation for antibacterial and antifungal activities. Eur. J. Med. Chem. 46, 4391–4402 (2011)

    Article  CAS  Google Scholar 

  6. J.-H. Jeon, T. Kakuta, K. Tanaka, Y. Chujo, Facile design of organicinorganic hybrid gels for molecular recognition of nucleoside triphosphates. Bioorgan. Med. Chem. Lett. 25, 2050–2055 (2015)

    Article  CAS  Google Scholar 

  7. F. Yoneda, Y. Sakuma, M. Ichiba, K. Shinomura, Syntheses of isoalloxazines and isoalloxazine 5-oxides. A new synthesis of riboflavin. J. Am. Chem. Soc. 98, 830–835 (1976)

    Article  CAS  Google Scholar 

  8. L.-S. Zhou, K.-W. Yang, L. Feng, J.-M. Xiao, C.-C. Liu, Y.-L. Zhang, M.W. Crowder, Novel fluorescent risedronates: synthesis, photodynamic inactivation and imaging of Bacillus subtilis. Bioorgan. Med. Chem. Lett. 23, 949–954 (2013)

    Article  CAS  Google Scholar 

  9. S. Ast, P.J. Rutledge, M.H. Todd, Reversing the triazole topology in a cyclam-triazole-dye ligand gives a 10-fold brighter signal response to Zn2+ in aqueous solution. Eur. J. Inorg. Chem. 2012, 5611–5615 (2012)

    Article  CAS  Google Scholar 

  10. T.-K. Kim, D.-N. Lee, H.-J. Kim, Highly selective fluorescent sensor for homocysteine and cysteine. Tetrahedron. Lett. 49, 4879–4881 (2008)

    Article  CAS  Google Scholar 

  11. L. Long, X. Li, D. Zhang, S. Meng, J. Zhang, X. Sun, C. Zhang, L. Zhou, L. Wang, Amino-coumarin based fluorescence ratiometric sensors for acidic pH and their application for living cells imaging. RSC Adv. 3, 12204–12209 (2013)

    Article  CAS  Google Scholar 

  12. J.I. Seeman, H.V. Secor, C.G. Chavdarian, E.B. Sanders, R.L. Bassfield, J.F. Whidby, Steric and conformational effects in nicotine chemistry. J. Organ. Chem. 46, 3040–3048 (1981)

    Article  CAS  Google Scholar 

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Correspondence to Amandeep Kaur .

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Kaur, A. (2018). Experimental Methods. In: Fluorescent Tools for Imaging Oxidative Stress in Biology. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-73405-7_10

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