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Amphipol-Assisted Cell-Free Expression of Membrane Proteins

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Membrane Proteins in Aqueous Solutions

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Summary

Cell-free expression of membrane proteins is a way to circumvent some of the problems encountered during their in vivo expression, among which is the small volume of membrane that is generally available for storing overexpressed membrane proteins and their toxicity when expressed in excessive amounts. Because of their mildness, amphipols appear as an attractive medium in which to solubilize and allow folding of membrane proteins expressed in vitro in cell lysates. Relatively few attempts at doing so have been described to date. They indicate (i) that all ionic polymers tested thus far interfere with the synthesis of α-helical membrane proteins and (ii) that two non-ionic polymers, NVoy and a glucosylated non-ionic amphipol, do allow their synthesis, folding, and solubilization in good yields.

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    Jean-Luc Popot

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Popot, JL. (2018). Amphipol-Assisted Cell-Free Expression of Membrane Proteins. In: Membrane Proteins in Aqueous Solutions. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-319-73148-3_7

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