Abstract
In many of biomedical applications, the implant might get in direct contact with the bone tissue where the osteogenesis needs to be stimulated. If osteoblasts can not successfully attach on the implant surface, the bone might resorb and implant can fail. In the current study MC3T3 cells were cultured on the 316L stainless steel samples which were deformed up to four different strain levels (5, 15, 25 and 35%) to activate plastic deformation mechanisms (slip and twinning) in different volume fractions. Scanning electron microscopy (SEM) images showed that cells adhered and spread significantly on the 25 and 35% deformed samples owing to the greater surface roughness and energy provided by the increased density of micro-deformation mechanisms which promoted the formation of focal contacts. In addition, significant amount of collagen formation was observed on the sample deformed up to 25% of strain which can be due to the ideal match of the surface roughness and collagen molecules. Overall these results show that material’s microstructure can be manipulated through plastic deformation mechanisms in order to enhance the cell response and collagen deposition. As a result long lasting implants could be obtained which would eliminate additional surgical interventions and provide a successful treatment.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chen Q, Thouas GA (2015) Mater Sci Eng R Reports 87:1–57
Uzer B, Toker SM, Cingoz A, Bagci-Onder T, Gerstein G, Maier HJ, Canadinc D (2016) J Mech Behav Biomed Mater 60:177–186
Anselme K (2000) Biomaterials 21:667–681
Le Guéhennec L, Soueidan A, Layrolle P, Amouriq Y (2007) Dent Mater 23:844–854
Linez-Bataillon P, Monchau F, Bigerelle M, Hildebrand HF (2002) Biomol Eng 19:133–141
Plecko M, Sievert C, Andermatt D, Frigg R, Kronen P, Klein K, Stübinger S, Nuss K, Bürki A, Ferguson S, Stoeckle U, Von Rechenberg B (2012) Bmc Musculoskelet Disord 1–12
Gronowicz G, McCarthy MB (1996) J Orthop Res 14:878–887
Deligianni DD, Katsala N, Ladas S, Sotiropoulou D, Amedee J, Missirlis YF (2001) Biomaterials 22:1241–1251
Qin L, Zeng Q, Wang W, Zhang Y, Dong G (2014) J Mater Sci 2662–2671
Meyer U, Büchter A, Wiesmann HP, Joos U, Jones DB (2005) Eur Cells Mater 9:39–49
Wexell CL (2013) Int J Biomater
Bordji K, Jouzeau JY, Mainard D, Payan E, Delagoutte JP, Netter P (1996) Biomaterials 17:491–500
Martinesi FBM, Bruni S, Stio M, Treves C, Bacci T (2006) J Biomed Mater Res A 81:131–145
Hayakawa T, Yoshida E, Yoshimura Y, Uo M, Yoshinari M (2012) Int J Biomaterials 1–7
Wu C, Chen M, Zheng T, Yang X (2015) Biomed Mat Eng 26:155–164
Arjonen A, Kaukonen R, Ivaska J (2011) Cell Adhes Migr 5:421–430
Mattila PK, Lappalainen P (2008) Nat Rev Mol Cell Biol 9:446–454
Kieswetter K, Schwartz Z, Hummert TW, Cochran DL, Simpson J, Dean DD, Boyan BD (1996) J Biomed Mater Res 32:55–63
Viguet-Carrin PDDS, Garnero P (2006) Osteoporos Int 17:319–336
Han J, Shin S, Herr Y, Kwon Y, Chung J (2011) Clin Oral Implant Res 1391–1398
De Barros RRM, Novaes AB Jr, Korn P, Queiroz A, De Almeida ALG, Hintze V, Scharnweber D, Bierbaum S, Stadlinger B (2013) Clin Implant Dent Relat Res 742–757
Acknowledgements
D. Canadinc acknowledges the financial support by the Turkish Academy of Sciences (TÜBA) within the Outstanding Young Scientist Program (GEBİP).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 The Minerals, Metals & Materials Society
About this paper
Cite this paper
Uzer, B., Monte, F., Awad, K.R., Aswath, P.B., Varanasi, V.G., Canadinç, D. (2018). The Influence of Plastic Deformation Mechanisms on the Adhesion Behavior and Collagen Formation in Osteoblast Cells. In: & Materials Society, T. (eds) TMS 2018 147th Annual Meeting & Exhibition Supplemental Proceedings. TMS 2018. The Minerals, Metals & Materials Series. Springer, Cham. https://doi.org/10.1007/978-3-319-72526-0_27
Download citation
DOI: https://doi.org/10.1007/978-3-319-72526-0_27
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-72525-3
Online ISBN: 978-3-319-72526-0
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)