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Cellular and Molecular Toxicology of Nanoparticles pp 215–226Cite as

Detection of DNA Damage Induced by Cerium Dioxide Nanoparticles: From Models to Molecular Mechanism Activated

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1048))

Abstract

This chapter will present an original effort to summarize the relevant data about the cyto-genotoxicity induced by cerium dioxide nanoparticles (nanoceria) in physiologically (in vivo and in vitro) relevant models. In this way, this chapter should be extremely useful to everyone who wants to plan their research and publishing their results. Massive application of nanoceria at different fields is increasing year after year, and it is urgent to address and discuss their use and its safety-related issues. Specifically, the nanoceria are being designed for nanomedicine, cosmetics, polishing materials and additives for automotive fuels. Their unique properties include the ability to absorb UV radiation, antioxidant potential and the rapid exchange of valence between Ce4+ and Ce3+ ions associated to oxygen storage. In this chapter, the state of the art regarding the physicochemical properties of nanoceria, nanogenotoxicity detected by in vitro and in vivo systems and the general aspects in the cyto-genotoxic mechanism of nanoceria are summarized. The cyto-genotoxicity will be discussed in terms of evaluations by Comet assay, Micronucleus test, DNA damage response and oxidative stress detected in cell culture systems and in vivo test. We also described the dose dependent cyto-genotoxic effects of nanoceria based on their physical-chemical nature. Paradoxically, these particles have been characterized as either pro-oxidant or anti-oxidant in dependence of microenvironment and physiological conditions such as pH. Finally, this chapter will contribute to point out aspects of the development of new in vitro and in vivo methodologies to detect cyto-genotoxic effects of the nanoceria.

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Authors and Affiliations

  1. Department of Genetics, Faculty of Medicine of Ribeirão Preto; USP, Ribeirão Preto, São Paulo, Brazil

    Tiago Alves Jorge de Souza

  2. Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiania, Goiás, Brazil

    Thiago Lopes Rocha

  3. Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto; USP, Ribeirão Preto, São Paulo, Brazil

    Leonardo Pereira Franchi

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  1. Tiago Alves Jorge de Souza
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  2. Thiago Lopes Rocha
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  3. Leonardo Pereira Franchi
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Correspondence to Leonardo Pereira Franchi .

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Editors and Affiliations

  1. Zoology Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Quaiser Saquib

  2. Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

  3. Zoology Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Abdulaziz A. Al-Khedhairy

  4. Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, Saudi Arabia

    Abdulrahman A. Alatar

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de Souza, T.A.J., Rocha, T.L., Franchi, L.P. (2018). Detection of DNA Damage Induced by Cerium Dioxide Nanoparticles: From Models to Molecular Mechanism Activated. In: Saquib, Q., Faisal, M., Al-Khedhairy, A., Alatar, A. (eds) Cellular and Molecular Toxicology of Nanoparticles. Advances in Experimental Medicine and Biology, vol 1048. Springer, Cham. https://doi.org/10.1007/978-3-319-72041-8_13

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