Abstract
Withania somnifera (L.) Dunal is one of the most important medicinal plant belonging to the family Solanaceae. Its root have been used as a drug since ancient times, and various pharmacological effects have been attributed to the occurrence of secondary compounds like withaferin-A and withanolide-A. Recently, huge interests are generated for production of these bioactive compounds through Agrobacterium rhizogenes-mediated hairy root culture techniques. The present review explores the culture conditions for efficient Agrobacterium-mediated hairy root culture system of W. somnifera for withanolide production. The hairy root induction is influenced by several factors like bacterial stain, type of explant, and cocultivation methods. The transformation efficiency could be enhanced by the addition of acetosyringone and SAAT treatments during cocultivation. Recent studies have also shown positive correlations of elicitors and biosynthetic pathway genes on withanolide production in hairy root culture of W. somnifera.
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Acknowledgments
Dr. A. Shajahan is thankful to the University Grants Commission (UGC), Govt. of India, New Delhi, for the financial support through UGC Major Research Projects (F. No. 33-174/2007 and F. No. 42-946/2013-SR). The authors also thank DST, Govt. of India, for providing facilities through DST-FIST program.
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Shajahan, A. et al. (2017). An Efficient Hairy Root System for Withanolide Production in Withania somnifera (L.) Dunal. In: Malik, S. (eds) Production of Plant Derived Natural Compounds through Hairy Root Culture . Springer, Cham. https://doi.org/10.1007/978-3-319-69769-7_7
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DOI: https://doi.org/10.1007/978-3-319-69769-7_7
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