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Conjugate Immunofluorescence—SEM Array Tomography for Studying Mammalian Synapses and Axons

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Cellular Imaging

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Abstract

Conjugate immunofluorescence—SEM array tomography enables the imaging of both the molecular content and the ultrastructure of tissues. The method is based on physical ultrathin serial sectioning, immunostaining and acquiring fluorescence and electron microscopy images of resin embedded tissues, followed by computational volume reconstruction and analysis. Conjugate immunofluorescence—SEM array tomography has been used for the study of brain tissue, and in particular for the characterization of diverse synapses and axons.

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Authors and Affiliations

  1. Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, 94305, USA

    Kristina D. Micheva

  2. Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC, 27599, USA

    Kristen D. Phend

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  1. Kristina D. Micheva
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Correspondence to Kristina D. Micheva .

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  1. Bio21 Institute Advanced Microscopy Facility, University of Melbourne, Melbourne, Victoria, Australia

    Eric Hanssen

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Micheva, K.D., Phend, K.D. (2018). Conjugate Immunofluorescence—SEM Array Tomography for Studying Mammalian Synapses and Axons. In: Hanssen, E. (eds) Cellular Imaging. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-319-68997-5_6

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