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Plant Respiration: Metabolic Fluxes and Carbon Balance pp 247–260Cite as

Respiratory Metabolism in Heterotrophic Plant Cells as Revealed by Isotopic Labeling and Metabolic Flux Analysis

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Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 43))

Summary

Mitochondrial respiration requires redox power that is mainly provided by the tricarboxylic acid (TCA) cycle in heterotrophic tissues. Glycolysis is commonly assumed to be the major pathway for carbon replenishment of the TCA cycle in most plant cells. However, the TCA cycle also provides precursors for amino acids and organic acids synthesis, a process that requires 4- or 5-C molecules supplied by anaplerotic pathways. The TCA cycle is thus involved in both anabolism and catabolism. Despite the good knowledge of enzymes and pathways involved in these processes, the regulation of carbon partitioning between catabolism and anabolism remains poorly understood. Metabolic flux analysis (MFA) aims at quantifying fluxes in metabolic networks and provides new insights for the study of the TCA cycle and associated pathways. This chapter presents briefly the principles of MFA, and describes how 14C-tracing evolved to 13C-MFA, and more recently to 13C-INST-MFA. Such analyses have provided insights about the origin of carbon atoms entering the TCA cycle and the partitioning between respiration and biosyntheses.

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Authors and Affiliations

  1. Biologie du Fruit et Pathologie, INRA, Université de Bordeaux, 33882, Villenave d’Ornon, UMR 1332, France

    Martine Dieuaide-Noubhani & Dominique Rolin

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  1. Martine Dieuaide-Noubhani
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  2. Dominique Rolin
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Correspondence to Martine Dieuaide-Noubhani .

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Editors and Affiliations

  1. Research School of Biology, College of Science, Australian National University, Canberra, Australia

    Guillaume Tcherkez

  2. Laboratoire d’Ecologie, Systématique et Evolution (ESE), UMR 8079, Université de Paris-Sud, CNRS, AgroParisTech, Université de Paris-Saclay, Orsay, France

    Jaleh Ghashghaie

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Dieuaide-Noubhani, M., Rolin, D. (2017). Respiratory Metabolism in Heterotrophic Plant Cells as Revealed by Isotopic Labeling and Metabolic Flux Analysis. In: Tcherkez, G., Ghashghaie, J. (eds) Plant Respiration: Metabolic Fluxes and Carbon Balance. Advances in Photosynthesis and Respiration, vol 43. Springer, Cham. https://doi.org/10.1007/978-3-319-68703-2_12

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