Ensuring Fresh Produce Safety and Quality by Utilizing Predictive Growth Models and Predictive Microbiology Software Tools

  • Shigenobu KosekiEmail author
Part of the Food Microbiology and Food Safety book series (FMFS)


Outbreaks of foodborne illnesses related to the consumption of fresh produce have been occurring all over the world. Quantitative microbial risk assessment (QMRA) for fresh produce will contribute to enhance microbiological safety of production, distribution, and consumption of fresh produce. This chapter describes the prevalence of contamination of fresh produce, possible route of pathogen contamination, and pathogen behavior on fresh produce during distribution. Predictive models and also web-based predictive tools described here will be useful for QMRA as an underpinning knowledge.


Fresh produce Predictive model Risk assessment Web-based tool 


  1. Abadias M, Usall J, Anguera M et al (2008) Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments. Int J Food Microbiol 123:121–129CrossRefPubMedGoogle Scholar
  2. Arthur L, Jones S, Fabri M, Odumeru J (2007) Microbial survey of selected Ontario-grown fresh fruits and vegetables. J Food Prot 70:2864–2867CrossRefPubMedGoogle Scholar
  3. Baranyi J, Roberts TA (1994) A dynamic approach to predicting bacterial-growth in food. Int J Food Microbiol 23:277–294CrossRefPubMedGoogle Scholar
  4. Baranyi J, Roberts TA (1995) Mathematics of predictive food microbiology. Int J Food Microbiol 26:199–218CrossRefPubMedGoogle Scholar
  5. Bohaychuk VM, Bradbury RW, Dimock R et al (2009) A microbiological survey of selected Alberta-grown fresh produce from farmers' markets in Alberta, Canada. J Food Prot 72:415–420CrossRefPubMedGoogle Scholar
  6. Bovill R, Bew J, Cook N et al (2000) Predictions of growth for Listeria monocytogenes and Salmonella during fluctuating temperature. Int J Food Microbiol 59:157–165CrossRefPubMedGoogle Scholar
  7. Bovill RA, Bew J, Baranyi J (2001) Measurements and predictions of growth for Listeria monocytogenes and Salmonella during fluctuating temperature. Int J Food Microbiol 67:131–137CrossRefPubMedGoogle Scholar
  8. Bovo F, De Cesare A, Manfreda G et al (2015) Fate of Salmonella enterica in a mixed ingredient salad containing lettuce, cheddar cheese, and cooked chicken meat. J Food Prot 78:491–497CrossRefPubMedGoogle Scholar
  9. Carrasco E, Perez-Rodriguez F, Valero A, Garcı RM (2008) Growth of Listeria monocytogenes on shredded, ready-to-eat iceberg lettuce. Food Control 19(5):487–494CrossRefGoogle Scholar
  10. Castillejo Rodrıguez AM, Barco Alcalá E, Garcıa Gimeno RM, Zurera Cosano G (2000) Growth modelling of Listeria monocytogenes in packaged fresh green asparagus. Food Microbiol 17:421–427CrossRefGoogle Scholar
  11. Doering HJ, Harrison MA, Morrow RA et al (2009) Use of the systems approach to determine the fate of Escherichia coli O157:H7 on fresh lettuce and spinach. J Food Prot 72:1560–1568CrossRefPubMedGoogle Scholar
  12. Harris LJ, Farber JN, Beuchat LR et al (2003) Outbreaks associated with fresh produce: incidence, growth, and survival of pathogens in fresh and fresh-cut produce. Comp Rev Food Sci Food Safety 2:78–141CrossRefGoogle Scholar
  13. Heaton JC, Jones K (2008) Microbial contamination of fruit and vegetables and the behaviour of enteropathogens in the phyllosphere: a review. J Appl Microbiol 104:613–626CrossRefPubMedGoogle Scholar
  14. Johannessen GS, Loncarevic S, Kruse H (2002) Bacteriological analysis of fresh produce in Norway. Int J Food Microbiol 77:199–204CrossRefPubMedGoogle Scholar
  15. Johnston LM, Jaykus L-A, Moll D et al (2005) A field study of the microbiological quality of fresh produce. J Food Prot 68:1840–1847CrossRefPubMedGoogle Scholar
  16. Johnston LM, Jaykus L-A, Moll D et al (2006) A field study of the microbiological quality of fresh produce of domestic and Mexican origin. Int J Food Microbiol 112:83–95CrossRefPubMedGoogle Scholar
  17. Koseki S (2009) Microbial Responses Viewer (MRV): a new ComBase-derived database of microbial responses to food environments. Int J Food Microbiol 134:75–82CrossRefPubMedGoogle Scholar
  18. Koseki S, Isobe S (2005a) Prediction of pathogen growth on iceberg lettuce under real temperature history during distribution from farm to table. Int J Food Microbiol 104:239–248CrossRefPubMedGoogle Scholar
  19. Koseki S, Isobe S (2005b) Growth of Listeria monocytogenes on iceberg lettuce and solid media. Int J Food Microbiol 101:217–225CrossRefPubMedGoogle Scholar
  20. Koseki S, Mizuno Y, Kawasaki S, Yamamoto K (2011) A survey of iceberg lettuce for the presence of Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes in Japan. J Food Prot 74:1543–1546CrossRefPubMedGoogle Scholar
  21. Likotrafiti E, Smirniotis P, Nastou A, Rhoades J (2013) Effect of relative humidity and storage temperature on the behavior of Listeria monocytogenes on fresh vegetables. J Food Saf 33:545–551CrossRefGoogle Scholar
  22. Loncarevic S, Johannessen GS, Rørvik LM (2005) Bacteriological quality of organically grown leaf lettuce in Norway. Lett Appl Microbiol 41:186–189CrossRefPubMedGoogle Scholar
  23. Luo Y, He Q, McEvoy JL (2010) Effect of storage temperature and duration on the behavior of Escherichia coli O157:H7 on packaged fresh-cut salad containing romaine and iceberg lettuce. J Food Sci 75:M390–M397CrossRefPubMedGoogle Scholar
  24. McKellar RC, Delaquis P (2011) Development of a dynamic growth–death model for Escherichia coli O157: H7 in minimally processed leafy green vegetables. Int J Food Microbiol 151:7–14CrossRefPubMedGoogle Scholar
  25. McKellar RC, LeBlanc DI, Lu J, Delaquis P (2012) Simulation of Escherichia coli O157:H7 behavior in fresh-cut lettuce under dynamic temperature conditions during distribution from processing to retail. Foodborne Pathog Dis 9:239–244CrossRefPubMedGoogle Scholar
  26. McMahon M, Wilson IG (2001) The occurrence of enteric pathogens and Aeromonas species in organic vegetables. Int J Food Microbiol 70:155–162CrossRefPubMedGoogle Scholar
  27. McMeekin TA, Olley J, Ross T, Ratkowsky DA (1993) Predictive microbiology: theory and application. Research Studies Press, Taunton, SomersetGoogle Scholar
  28. Mukherjee A, Speh D, Dyck E (2004) Preharvest evaluation of coliforms, Escherichia coli, Salmonella, and Escherichia coli O157: H7 in organic and conventional produce grown by Minnesota farmers. J Food Prot 67:894–900CrossRefPubMedGoogle Scholar
  29. Mukherjee A, Speh D, Jones AT et al (2006) Longitudinal microbiological survey of fresh produce grown by farmers in the upper midwest. J Food Prot 69:1928–1936CrossRefPubMedGoogle Scholar
  30. Posada-Izquierdo GD, Perez-Rodriguez F, López-Gálvez F et al (2013) Modelling growth of Escherichia coli O157:H7 in fresh-cut lettuce submitted to commercial process conditions: chlorine washing and modified atmosphere packaging. Food Microbiol 33:131–138CrossRefPubMedGoogle Scholar
  31. Posada-Izquierdo GD, Perez-Rodriguez F, López-Gálvez F et al (2014) Modeling growth of Escherichia coli O157:H7 in fresh-cut lettuce treated with neutral electrolyzed water and under modified atmosphere packaging. Int J Food Microbiol 177:1–8CrossRefPubMedGoogle Scholar
  32. Riva M, Franzetti L, Galli A (2001) Microbiological quality and shelf life modeling of ready-to-eat cicorino. J Food Prot 64:228–234CrossRefPubMedGoogle Scholar
  33. Sagoo SK, Little CL, Mitchell RT (2001) The microbiological examination of ready-to-eat organic vegetables from retail establishments in the United Kingdom. Lett Appl Microbiol 33:434–439CrossRefPubMedGoogle Scholar
  34. Sagoo SK, Little CL, Mitchell RT (2003) Microbiological quality of open ready-to-eat salad vegetables: effectiveness of food hygiene training of management. J Food Prot 66:1581–1586CrossRefPubMedGoogle Scholar
  35. Sant'Ana AS, Barbosa MS, Destro MT et al (2012a) Growth potential of Salmonella spp. and Listeria monocytogenes in nine types of ready-to-eat vegetables stored at variable temperature conditions during shelf-life. Int J Food Microbiol 157:52–58CrossRefPubMedGoogle Scholar
  36. Sant'Ana AS, Franco BDGM, Schaffner DW (2012b) Modeling the growth rate and lag time of different strains of Salmonella enterica and Listeria monocytogenes in ready-to-eat lettuce. Food Microbiol 30:267–273CrossRefPubMedGoogle Scholar
  37. Shorten PR, Membre JM, Pleasants AB et al (2004) Partitioning of the variance in the growth parameters of Erwinia carotovora on vegetable products. Int J Food Microbiol 93:195–208CrossRefPubMedGoogle Scholar
  38. Sivapalasingam S, Friedman CR, Cohen L, Tauxe RV (2004) Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. J Food Prot 67:2342–2353CrossRefPubMedGoogle Scholar
  39. Tenenhaus-Aziza F, Ellouze M (2015) Software for predictive microbiology and risk assessment: a description and comparison of tools presented at the ICPMF8 Software Fair. Food Microbiol 45:290–299CrossRefPubMedGoogle Scholar
  40. Theofel CG, Harris LJ (2009) Impact of preinoculation culture conditions on the behavior of Escherichia coli O157:H7 inoculated onto romaine lettuce (Lactuca sativa) plants and cut leaf surfaces. J Food Prot 72:1553–1559CrossRefPubMedGoogle Scholar
  41. Viswanathan P, Kaur R (2001) Prevalence and growth of pathogens on salad vegetables, fruits and sprouts. Int J Hyg Environ Health 203:205–213CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.Hokkaido UniversitySapporoJapan

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