Abstract
Butanol tolerance is a critical factor affecting the ability of microorganisms to produce economically viable quantities of butanol through acetone-butanol-ethanol (ABE) fermentation using renewable feedstocks. However, ABE process has certain challenges like maintaining strict anaerobic conditions, slow growth rate of microorganisms, the rapid shift of pH, sensitivity to acetic acid, low butanol titer, solvent tolerance, and product inhibition. Separation of fermentation products through distillation, gas stripping, pervaporation, and adsorption also makes the process costly. Despite their importance at a biofuel platform, a limited number of butanol-tolerant bacteria have been identified so far. This problem can be eradicated through the isolation of solvent tolerating bacteria, development of bacteria through evolutionary engineering, mutation, and genetic engineering with promising product recovery techniques. In the present chapter, an overview of the butanol tolerating microbes, their solvent survival strategies, and the techniques to overcome the problem for a high concentration of butanol have been discussed.
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Acknowledgements
Authors acknowledge the grant support from Science & Engineering Research Board, New Delhi, Govt. of India (File No. YSS/2015/000295). Authors also thank to Sardar Swaran Singh National Institute of Bio-Energy, Kapurthala, India as the host Institution for providing laboratory space to complete this work.
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Behera, S., Sharma, N.K., Kumar, S. (2018). Prospects of Solvent Tolerance in Butanol Fermenting Bacteria. In: Kumar, S., Sani, R. (eds) Biorefining of Biomass to Biofuels. Biofuel and Biorefinery Technologies, vol 4. Springer, Cham. https://doi.org/10.1007/978-3-319-67678-4_11
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