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Perspectives in Immunology of Wound Healing

  • Kai Masur
  • Sander Bekeschus
Chapter

Abstract

Cold plasmas are partially ionised gases at low temperatures containing biologically active components—mostly reactive oxygen species (ROS), which also play an essential role in natural processes such as immune defence. Furthermore, those plasma generated ROS act commonly with other plasma components such as UV radiation, mild heat and electrical fields. Cold plasmas influence the cellular redox balance and can be adjusted depending on composition and treatment time so that cells are either stimulated or harmed. However, the sensitivities of the treated cells differ greatly from one another—which is dependent on different antioxidative potentials of the different cell types, as well as their ability to regenerate. Cold plasmas are useful for killing bacteria—multiresistant pathogens as well as non-resistant strains. It has been shown that a balanced plasma treatment of human cells can also lead to their stimulation. Therefore, a well-adjusted plasma composition can mediate an improved microenvironment by generating bactericidal conditions as well as positively influencing microcirculation of the plasma treated tissues. Furthermore, cold plasma treatment promotes an increased oxygen saturation and an improved supply with nutrition. Therefore, cold plasmas are useful to support wound healing by accelerating cell proliferation and triggering immune processes. This chapter will discuss the parallels between immune system responses and the effects of cold atmospheric pressure plasmas on cells and tissues.

Keywords

Cold plasma Reactive oxygen species Wound healing Oxidative burst 

References

  1. 1.
    Mills CD. M1 and M2 macrophages: oracles of health and disease. Crit Rev Immunol. 2012;32(6):463–88.CrossRefGoogle Scholar
  2. 2.
    Mills CD. Anatomy of a discovery: M1 and M2 macrophages. Front Immunol. 2015;6:212.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Tresp H, Hammer MU, Weltmann K-D, Reuter S. Effects of atmosphere composition and liquid type on plasma-generated reactive species in biologically relevant solutions. Plasma Med. 2013;3(1–2):45–55.CrossRefGoogle Scholar
  4. 4.
    Tresp H, Hammer MU, Winter J, Weltmann KD, Reuter S. Quantitative detection of plasma-generated radicals in liquids by electron paramagnetic resonance spectroscopy. J Phys D Appl Phys. 2013;46(43):435401.CrossRefGoogle Scholar
  5. 5.
    Arjunan KP, Clyne AM. Hydroxyl radical and hydrogen peroxide are primarily responsible for dielectric barrier discharge plasma-induced angiogenesis. Plasma Process Polym. 2011;8(12):1154–64.CrossRefGoogle Scholar
  6. 6.
    Von Woedtke T, Haese K, Heinze J, Oloff C, Stieber M, Julich WD. Sporicidal efficacy of hydrogen peroxide aerosol. Pharmazie. 2004;59(3):207–11.Google Scholar
  7. 7.
    Bekeschus S, Kolata J, Winterbourn C, Kramer A, Turner R, Weltmann KD, Broker B, Masur K. Hydrogen peroxide: a central player in physical plasma-induced oxidative stress in human blood cells. Free Radic Res. 2014;48(5):542–9.CrossRefGoogle Scholar
  8. 8.
    Bundscherer L, Nagel S, Hasse S, Tresp H, Wende K, Walther R, Reuter S, Weltmann KD, Masur K, Lindequist U. Non-thermal plasma treatment induces MAPK signaling in human monocytes. Open Chem. 2015;13(1):606–13.Google Scholar
  9. 9.
    Haertel B, Volkmann F, von Woedtke T, Lindequist U. Differential sensitivity of lymphocyte subpopulations to non-thermal atmospheric-pressure plasma. Immunobiology. 2012;217(6):628–33.CrossRefGoogle Scholar
  10. 10.
    Barton A, Hasse S, Bundscherer L, Wende K, Weltmann K, Lindequist U, Masur K. Growth factors and cytokines are regulated by non-thermal atmospheric pressure plasma. Exp Dermatol. 2014;23(3):E7.Google Scholar
  11. 11.
    Niethammer P, Grabher C, Look AT, Mitchison TJ. A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish. Nature. 2009;459(7249):996–9.CrossRefGoogle Scholar
  12. 12.
    Loo AE, Wong YT, Ho R, Wasser M, Du T, Ng WT, Halliwell B. Effects of hydrogen peroxide on wound healing in mice in relation to oxidative damage. PLoS One. 2012;7(11):e49215.CrossRefGoogle Scholar
  13. 13.
    Barekzi N, Laroussi M. Effects of low temperature plasmas on cancer cells. Plasma Process Polym. 2013;10(12):1039–50.CrossRefGoogle Scholar
  14. 14.
    Hori M, Laroussi M, Masur K, Ikehara Y. Plasma processes and cancer—special topical cluster of the 2nd IWPCT meeting. Plasma Process Polym. 2015;12(12):1336–7.CrossRefGoogle Scholar
  15. 15.
    Keidar M, Shashurin A, Volotskova O, Stepp MA, Srinivasan P, Sandler A, Trink B. Cold atmospheric plasma in cancer therapy. Phys Plasmas. 2013;20(5):057101–8.CrossRefGoogle Scholar
  16. 16.
    Nagendra Kumar K, Neha K, Booki M, Ki Hong C, Young June H, Vandana M, Alexander F, Eun Ha C. Cytotoxic macrophage-released tumour necrosis factor-alpha (TNF- α ) as a killing mechanism for cancer cell death after cold plasma activation. J Phys D Appl Phys. 2016;49(8):084001.CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Associated Research Group “Plasma Wound Healing”Centre for Innovation Competence (ZIK) PlasmatisGreifswaldGermany
  2. 2.Young Research Group “Plasma Redox Effects”Centre for Innovation Competence (ZIK) PlasmatisGreifswaldGermany

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