Abstract
Proteins from TCTP/HRF family were identified as venom toxins of spiders from different genus. We have found a TCTP toxin in the venom gland of Loxosceles intermedia, a venomous spider very common in South Brazil. TCTP from L. intermedia, named LiTCTP, was cloned, produced in a heterologous prokaryotic system, and the recombinant toxin was biochemically characterized. Our results point that LiTCTP is involved in the inflammatory events of Loxocelism, the clinical signs triggered after Loxosceles sp. bite, which include intense inflammatory reaction at the bite site followed by local necrosis. TCTP toxins were also identified in spiders from different genus. There are very few articles about TCTP toxins in other venomous animals in the literature, although a NCBI database search on the protein sequences reveals TCTP on snake’s venom glands transcriptomic and genomic studies. Studies on TCTP as a venom toxin are very few and its biological role as a venom component in prey capture is still unknown.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Buch DR, Souza FN, Meissner GO, Morgon AM, Gremski LH, Ferrer VP, Trevisan-Silva D, Matsubara FH, Boia-Ferreira M, Sade YB, Chaves-Moreira D, Gremski W, Veiga SS, Chaim OM, Senff-Ribeiro A (2015) Brown spider (Loxosceles genus) venom toxins: evaluation of biological conservation by immune cross-reactivity. Toxicon 108:154–166
da Silva PH, da Silveira RB, Appel MH, Mangili OC, Gremski W, Veiga SS (2004) Brown spiders and Loxoscelism. Toxicon 44:693–709
dos Santos VL, Franco CR, Viggiano RL, da Silveira RB, Cantao MP, Mangili OC, Veiga SS, Gremski W (2000) Structural and ultrastructural description of the venom gland of Loxosceles intermedia (brown spider). Toxicon 38:265–285
Escoubas P, Rash L (2004) Tarantulas: eight-legged pharmacists and combinatorial chemists. Toxicon 43(5):555–574
Gremski LH, Da Silveira RB, Chaim OM, Probst CM, Ferrer VP, Nowatzki J, Weinschutz HC, Madeira HM, Gremski W, Nader HB, Senff-Ribeiro A, Veiga SS (2010) A novel expression profile of the Loxosceles intermedia spider venomous gland revealed by transcriptome analysis. Mol BioSyst 6(12):2403–2416
Gremski LH, Trevisan-Silva D, Ferrer VP, Matsubara FH, Meissner GO, Wille AC, Vuitika L, Dias-Lopes C, Ullah A, De Moraes FR, Chávez-Olórtegui C, Barbaro KC, Murakami MT, Arni RK, Senff-Ribeiro A, Chaim OM, Veiga SS (2014) Recent advances in the understanding of brown spider venoms: from the biology of spiders to the molecular mechanisms of toxins. Toxicon 83:91–120
Kimura T, Ono S, Kubo T (2012) Molecular cloning and sequence analysis of the cDNAs encoding toxin-like peptides from the venom glands of tarantula Grammostola rosea. Int J Peptides 2012:731293. https://doi.org/10.1155/2012/731293
MacDonald SM (2012) Potential role of histamine releasing factor (HRF) as a therapeutic target for treating asthma and allergy. J Asthma Allergy 5:51–59
Margres MJ, Aronow K, Loyacano J, Rokyta DR (2013) The venom-gland transcriptome of the eastern coral snake (Micrurus fulvius) reveals high venom complexity in the intragenomic evolution of venoms. BMC Genomics 14:531. https://doi.org/10.1186/1471-2164-14-531
McGivern JJ, Wray KP, Margres MJ, Couch ME, Mackessy SP, Rokyta DR (2014) RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes. BMC Genomics 15:1061. https://doi.org/10.1186/1471-2164-15-1061
Mulenga A, Azad AF (2005) The molecular and biological analysis of ixodid ticks histamine release factors. Exp Appl Acarol 37:215–229
Paludo KS, Biscaia SM, Chaim OM, Otuki MF, Naliwaiko K, Dombrowski PA, Franco CR, Veiga SS (2009) Inflammatory events induced by brown spider venom and its recombinant dermonecrotic toxin: a pharmacological investigation. Comp Biochem Physiol C Toxicol Pharmacol 149(3):323–333
Platinick NI (2014) The world spider catalog. American Museum of Natural History, New York, NY. Version 14.5
Rattmann YD, Pereira CR, Cury Y, Gremski W, Marques MC, da Silva-Santos JE (2008) Vascular permeability and vasodilation induced by the Loxosceles intermedia venom in rats: involvement of mast cell degranulation, histamine and 5-HT receptors. Toxicon 51(3):363–372
Ribeiro RO, Chaim OM, da Silveira RB, Gremski LH, Sade YB, Paludo KS, Senff-Ribeiro A, de Moura J, Chávez-Olórtegui C, Gremski W, Nader HB, Veiga SS (2007) Biological and structural comparison of recombinant phospholipase D toxins from Loxosceles intermedia (brown spider) venom. Toxicon 50(8):1162–1174
Rokyta DR, Lemmon AR, Margres MJ, Aronow K (2012) The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus). BMC Genomics 13:312. https://doi.org/10.1186/1471-2164-13-312
Rokyta DR, Wray KP, Margres MJ (2013) The genesis of an exceptionally lethal venom in the timber rattlesnake (Crotalus horridus) revealed through comparative venom-gland transcriptomics. BMC Genomics 14:394. https://doi.org/10.1186/1471-2164-14-394
Sade YB, Bóia-Ferreira M, Gremski LH, Da Silveira RB, Gremski W, Senff-Ribeiro A, Chaim OM, Veiga SS (2012) Molecular cloning, heterologous expression and functional characterization of a novel translationally-controlled tumor protein (TCTP) family member from Loxosceles intermedia (brown spider) venom. Int J Biochem Cell Biol 44(1):170–177
Sun J, Wu Y, Wang J, Ma F, Liu X, Li Q (2008) Novel translationally controlled tumor protein homologue in the buccal gland secretion of Lampetra japonica. Biochimie 90:1760–1768
Vetter RS (2008) Spiders of the genus Loxosceles (Araneae, Sicariidae): a review of biological, medical and psychological aspects regarding envenomations. J Arachnol 36:150–163
Zhang Y, Chen J, Tang X, Wang F, Jiang L, Xiong X, Wang M, Rong M, Liu Z, Liang S (2010) Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosa singoriensis. Zoology (Jena) 113(1):10–18. https://doi.org/10.1016/j.zool.2009.04.001
Zobel-Thropp PA, Correa SM, Garb JE, Binford GJ (2014) Spit and venom from Scytodes spiders: a diverse and distinct cocktail. J Proteome Res 13:817–835. https://doi.org/10.1021/pr400875s
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this chapter
Cite this chapter
Senff-Ribeiro, A. (2017). Translationally Controlled Tumor Protein (TCTP/HRF) in Animal Venoms. In: Telerman, A., Amson, R. (eds) TCTP/tpt1 - Remodeling Signaling from Stem Cell to Disease. Results and Problems in Cell Differentiation, vol 64. Springer, Cham. https://doi.org/10.1007/978-3-319-67591-6_9
Download citation
DOI: https://doi.org/10.1007/978-3-319-67591-6_9
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-67590-9
Online ISBN: 978-3-319-67591-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)