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Three-Dimensional Tissue Models and Available Probes for Multi-Parametric Live Cell Microscopy: A Brief Overview

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Multi-Parametric Live Cell Microscopy of 3D Tissue Models

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1035))

Abstract

In recent years, the advances in tissue engineering and regenerative medicine have resulted in introduction of novel 3D tissue models, materials and methods to the regular practice of cell biologists, material scientists and specialists from related areas. 3D tissue models allow mimicking in vivo cell and tissue organization. However, the efficient work in three dimensions has significant challenges, such as compatibility with conventional cell biology methods, live cell imaging and quantification readouts. Here, we briefly discuss the applicability of 3D tissue models to different live cell microscopy modalities and the available range of fluo- and phosphorescent probes and sensors allowing for multi-parametric imaging.

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Acknowledgments

This work was supported by Science Foundation Ireland (SFI) grant 13/SIRG/2144. We thank Prof. D. Papkovsky for useful comments on the manuscript.

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  1. Metabolic Imaging Group, School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland

    Neil O’Donnell & Ruslan I. Dmitriev

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  1. Neil O’Donnell
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Correspondence to Ruslan I. Dmitriev .

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    Ruslan I. Dmitriev

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O’Donnell, N., Dmitriev, R.I. (2017). Three-Dimensional Tissue Models and Available Probes for Multi-Parametric Live Cell Microscopy: A Brief Overview. In: Dmitriev, R. (eds) Multi-Parametric Live Cell Microscopy of 3D Tissue Models. Advances in Experimental Medicine and Biology, vol 1035. Springer, Cham. https://doi.org/10.1007/978-3-319-67358-5_4

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