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Cryopreservation of Cells Encapsulated Within Nano-thin Polyelecrolyte Coatings

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Recent Developments and Achievements in Biocybernetics and Biomedical Engineering (PCBBE 2017)

Part of the book series: Advances in Intelligent Systems and Computing ((AISC,volume 647))

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Abstract

Cryopreservation is a method which enables to store the cells for a long time period and allows to obtain the appropriate amount of cells necessary for transplantation. Unfortunately, the cells isolated from organs like e.g. hepatocytes are susceptible to freezing damage. Encapsulation may be considered as a method allowing to protect cells during adverse freezing conditions.

Aim: Assesement of the usability of nano-thin semipermeable membrane coating shell as protective element during cryopreservation of the cells.

Materials and Methods: Liver cells, isolated from living donors (according to Bioethical Community protocol) or hepatoma cell line HepG2 were encapsulated within nano-thin poly-L-lysine/polyethylenoimine with incorporated fullerene (PLL/PEI+f) membrane or within standard alginate microcapsules. As a control group the cells were encapsulated The mitochondrial activity of cells was analyzed after 4 months cryopreservation using 5-diphenyltertrazolium bromide tetrazolium (MTT) test. The viability of cells was assessed utilizing flow cytometry during 8-day culture after thawing.

Results: The cells protected during cryopreservation by nanomembranes or microcapsules were after thawing in better condition than nonencapsulated cells. The mitochondrial activity expressed as absorbance was comparable in both types of encapsulation within nano-thin membranes or microcapsules in MTT test. The percentage share of viable cells in all tested groups was meanly 90%. Conclusions: Applied nanocoating did not delimit the viability of cryopreserved cells as compared to microencapsulation. The cryopreservation within nano-thin semipermeable membranes seems to be promising way to protect cells during long term storage in liquid nitrogen when minimizing the encapsulated transplant volume is necessary.

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Authors and Affiliations

  1. Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Science, Warsaw, Poland

    Magdalena Antosiak-Iwańska, Ewa Godlewska, Joanna Kinasiewicz, Jerzy Kawiak & Ludomira Granicka

  2. Department of General, Transplant and Liver Surgery, Medical University of Warsaw, Warsaw, Poland

    Krzysztof Dudek

Authors
  1. Magdalena Antosiak-Iwańska
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  2. Ewa Godlewska
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  3. Joanna Kinasiewicz
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  4. Krzysztof Dudek
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  5. Jerzy Kawiak
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  6. Ludomira Granicka
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Correspondence to Magdalena Antosiak-Iwańska .

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Editors and Affiliations

  1. AGH University of Science and Technology, Krakow, Poland

    Piotr Augustyniak

  2. Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland

    Roman Maniewski

  3. AGH University of Science and Technology, Krakow, Poland

    Ryszard Tadeusiewicz

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Antosiak-Iwańska, M., Godlewska, E., Kinasiewicz, J., Dudek, K., Kawiak, J., Granicka, L. (2018). Cryopreservation of Cells Encapsulated Within Nano-thin Polyelecrolyte Coatings. In: Augustyniak, P., Maniewski, R., Tadeusiewicz, R. (eds) Recent Developments and Achievements in Biocybernetics and Biomedical Engineering. PCBBE 2017. Advances in Intelligent Systems and Computing, vol 647. Springer, Cham. https://doi.org/10.1007/978-3-319-66905-2_21

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  • DOI: https://doi.org/10.1007/978-3-319-66905-2_21

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