Abstract
The article presents a complex method of recognition nuclei cells areas and of segmentation of nuclei. The evaluation process of the identification and segmentation quality of proposed methods using L2 distance function and sensitivity function is also presented. FISH test is a fluorescence technique used for staining of microscope images of breast cancer. The technique allows visualization of HER2, CEN17 genes and cells nuclei. Fast and efficient microscopy image analysis allows a proper choice of therapy. This article presents a new, complex technique based on the color analysis, morphological transformations and watershed segmentation. The technique allows rapid and efficient identification of nuclei areas, as well as precise detection of the cells nuclei outlines. This step is often overlooked in a computer image analysis, whereas it is extremely important. It allows to increase the accuracy of HER2/CEN17 gene detection, as well as it allows to exclude fake biomarkers and increase the speed of identification of algorithms for HER2 genes by limiting the searched area. Proper segmentation of nuclei also makes manual evaluation of images easier.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Dako HER2 FISH pharmDx, Kit Nr kat. K5331, Wydanie czwarte
Kasampalidis, J.N., Pitas, I., Karayannopolou, G.: FISH image analysis using a modified radial basis function network. In: Signals, Circuits and Systems ISSCS, vol. 2, pp. 1–4 (2007)
Les, T., Markiewicz, T., Osowski, S., Cichowicz, M., Kozlowski, W.: Automatic evaluation system of FISH images in breast cancer. In: Elmoataz, A., Lezoray, O., Nouboud, F., Mammass, D. (eds.) Image and Signal Processing. LNCS, vol. 8509, pp. 332–339. Springer, Cham (2014)
Les, T., Markiewicz, T., Osowski, T., Jesiotr, M., Kozlowski, W.: Localization of spots in FISH images of breast cancer using 3-D shape analysis. J. Microsc. 262(3), 252–259 (2016). http://dx.doi.org/10.1111/jmi.12360
Les, T., Markiewicz, T., Osowski, S., Kozlowski, W., Jesiotr, M.: Fusion of FISH image analysis methods of HER2 status determination in breast cancer. Expert Syst. Appl. (2016). doi:10.1016/j.eswa.2016.05.020
Esa, A., Trakhtenbrot, L., Hausmann, M.: Fast-FISH detection and semi-automated image analysis of numerical chromosome aberrations in hematological malignancies. Anal. Cell. Pathol. 16, 211–22 (1998)
Clocksin, W.F., Lerner, B.: Automatic analysis of fluorescence in situ hybridization images. In: Proceedings of the 11th British Machine Vision Conference, Bristol, England, pp. 666–674, September 2000
Beucher, S.: The Watershed Transformation Applied to Image Segmentation. Ecole des Mines de Paris (2000)
Beucher, S.: Watersheds and Waterfalls. Cours Ecole d’Eté de Morphologie Mathématique
Lerner, B., Clocksin, W.F., Dhanjal, S., Hulten, M.A., Bishop, C.M.: Feature representation and signal classification in fluorescence in-situ hybridization image analysis. IEEE Trans. Syst. Man Cybern. 31, 655–665 (2001)
Solorzano, C.O., Santos, A., Vallcorba, I., Garcia-Sagredo, J.-M., Pozo, F.: Automated FISH spot counting in interphase nuclei: statistical validation and data correction. Cytometry 31(2), 93–99 (1998)
Pratt, W.K.: Digital Image Processing. Wiley, New York (1991)
Gonzalez, R.C.G., Woods, R.E.: Digital Image Processing. Prentice Hall, New Jersey (1992)
Acknowledgment
This work has been supported by the National Science Centre (2012/07/B/ST7/01203 grant), Poland.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG
About this paper
Cite this paper
Les, T., Markiewicz, T., Patera, J. (2018). Automatic Detection of Cells in FISH Images Using Map of Colors and Three-Track Segmentation. In: Augustyniak, P., Maniewski, R., Tadeusiewicz, R. (eds) Recent Developments and Achievements in Biocybernetics and Biomedical Engineering. PCBBE 2017. Advances in Intelligent Systems and Computing, vol 647. Springer, Cham. https://doi.org/10.1007/978-3-319-66905-2_11
Download citation
DOI: https://doi.org/10.1007/978-3-319-66905-2_11
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-66904-5
Online ISBN: 978-3-319-66905-2
eBook Packages: EngineeringEngineering (R0)