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Cellular and Molecular Approaches to Regeneration and Repair pp 239–257Cite as

Combination Treatment of Mesenchymal Stem Cells (MSCs) and Angelica sinensis’ Active Ingredients for Ischemic Stroke

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Part of the book series: Springer Series in Translational Stroke Research ((SSTSR))

Abstract

At present, mesenchymal stem cells (MSCs) are regarded as a candidate for neovascularization and tissue regeneration after ischemic stroke. Numerous studies reported that Angelica (also called Dong quai, a well-known Chinese herbal medicine) extracts and its active ingredients such as ligustilide, n-Butylphthalide and sodium ferulate had significant effects of anti-inflammatory, anti-activation of oxygen free radicals, angiogenesis, anti-platelet aggregation, neuroprotection and so on. Angelica’ active compositions facilitated MSCs to migrate into infarcted zone and differentiation. Moreover, MSCs combined with angelica’ active components improved neurological function and decreased infarcted volume, advanced neovascularization and neurogenesis, regulated astrocytes characteristics, enhanced regional cerebral blood flow and glucose metabolism, as well as reduced brain-blood barrier permeability in infarction. Consequently, the structure and function of neurovascular unit in infarct region partly obtained recovery. Therefore, the combination treatment was a valuable therapy aimed at improving post-stroke restoration.

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Abbreviations

BBB:

Blood-brain barrier

BDNF:

Brain-derived neurotrophic factor

BMP:

Bone morphogenetic proteins

BP:

n-Butylidenephthalide

BrdU:

5-Bromo-2′-deoxyuridine

CBF:

Cerebral blood flow

CXCR4:

Chemokine (CXC motif) receptor-4

DCX:

Doublecortin

DG:

Dentate gyrus

EC:

Endothelial cell

ERK:

Extracellular signal-regulated kinases

FA:

Ferulic acid

FDA:

Food and Drug Administration

FDG:

18F-2-deoxy-glucose

GDNF:

Glial cell line-derived neurotrophic factor

GFAP:

Glial fibrillary acidic protein

HIF:

Hypoxia-inducible factors

HUVEC:

Human umbilical vein endothelial cell

MCAo:

Middle cerebral artery occlusion

MRI:

Magnetic resonance imaging

MSC:

Mesenchymal stem cell

mTOR:

Mammalian target of rapamycin

NBP:

n-Butylphthalide

PET/CT:

Positron emission tomography–computed tomography

PWI:

Perfusion-weighted imaging

SDF-1:

Stromal cell-derived factor-1

SF:

Sodium ferulate

STEPS:

Stem Cells as an Emerging Paradigm in Stroke

SVZ:

Subventricular zone

TTC:

2,3,5-Triphenyltetrazolium chloride

Tuj-1:

Neuron-specific class III beta-tubulin

VEGF:

Vascular endothelial growth factor

vWF:

Von Willebrand factor

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Authors and Affiliations

  1. State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, 999078, China

    Qian Zhang & Yonghua Zhao

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  1. Qian Zhang
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  2. Yonghua Zhao
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Correspondence to Yonghua Zhao .

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Editors and Affiliations

  1. Department of Neurology & Neurosurgery, Cedars-Sinai Medical Center, AHSP, Los Angeles, California, USA

    Paul A. Lapchak

  2. Department of Physiology, Loma Linda University, Loma Linda, California, USA

    John H. Zhang

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Zhang, Q., Zhao, Y. (2018). Combination Treatment of Mesenchymal Stem Cells (MSCs) and Angelica sinensis’ Active Ingredients for Ischemic Stroke. In: Lapchak, P., Zhang, J. (eds) Cellular and Molecular Approaches to Regeneration and Repair. Springer Series in Translational Stroke Research. Springer, Cham. https://doi.org/10.1007/978-3-319-66679-2_12

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  • DOI: https://doi.org/10.1007/978-3-319-66679-2_12

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  • Online ISBN: 978-3-319-66679-2

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