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Chlamydomonas reinhardtii: Protein Glycosylation and Production of Biopharmaceuticals

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Chlamydomonas: Biotechnology and Biomedicine

Part of the book series: Microbiology Monographs ((MICROMONO,volume 31))

Abstract

Recently, microalgae species such as Chlamydomonas reinhardtii have been investigated as potential biofactories for the production of biopharmaceuticals (Mathieu-Rivet et al., Front Plant Sci 5:359, 2014; Rasala and Mayfield, Photosynth Res 123:227–239, 2015). Biopharmaceuticals are protein-based pharmaceuticals which are produced recombinantly in living cells used as biofactories (Walsh, Nat Biotechnol 28:917–924, 2010; Walsh, Nat Biotechnol 32:992–1000, 2014). The pharmaceutical market encompasses more than 200 biopharmaceutical products (Walsh, Nat Biotechnol 32:992–1000, 2014). Most of these biopharmaceuticals are glycosylated proteins, and it is currently well established that their glycosylation is primordial for their stability, half-life, and biological activity (Walsh, Nat Biotechnol 28:917–924, 2010; Lingg et al., Biotechnol J, 7:1462–1472, 2012). Since enzymes involved in the glycosylation processing are localized in the endoplasmic reticulum and the Golgi apparatus, biopharmaceuticals produced in C. reinhardtii must travel through these organelles, which are components of the secretory pathway, to acquire an appropriate glycosylation. In this chapter, the C. reinhardtii protein glycosylation pathways as well as its capacity to synthesize and transport nucleotide sugars will be described and discussed.

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Acknowledgments

The authors are indebted to all coworkers and students at the Glyco-MEV laboratory who are currently contributing to the microalgae research project or did so in the past. They acknowledge Magda Dudek for careful reading of the manuscript. They are also thankful to the University of Rouen, the region Haute-Normandie now called Normandie and the IUF for their financial support.

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Authors and Affiliations

  1. Laboratoire Glycobiologie et Matrice Extracellulaire végétale, Normandie Univ, UNIROUEN, 76000, Rouen, France

    Elodie Mathieu-Rivet, Patrice Lerouge & Muriel Bardor

  2. Institut Universitaire de France (I.U.F.), 1 rue Descartes, 75231, Paris Cedex 05, France

    Muriel Bardor

  3. Faculté des sciences et techniques, Laboratoire Glycobiologie et Matrice Extracellulaire végétale (Glyco-MEV) Equipe d’Accueil 4358, Institut de Recherche et d’Innovation Biomédicale, Végétale Agronomie Sol Innovation, Université de Rouen, Normandie Université, 76821, Mont-Saint-Aignan Cedex, France

    Muriel Bardor

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  1. Elodie Mathieu-Rivet
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  2. Patrice Lerouge
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  3. Muriel Bardor
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Correspondence to Muriel Bardor .

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  1. Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany

    Michael Hippler

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Mathieu-Rivet, E., Lerouge, P., Bardor, M. (2017). Chlamydomonas reinhardtii: Protein Glycosylation and Production of Biopharmaceuticals. In: Hippler, M. (eds) Chlamydomonas: Biotechnology and Biomedicine. Microbiology Monographs, vol 31. Springer, Cham. https://doi.org/10.1007/978-3-319-66360-9_3

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