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An Introduction to Lensless Digital Holographic Microscopy

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Miniature Fluidic Devices for Rapid Biological Detection

Part of the book series: Integrated Analytical Systems ((ANASYS))

Abstract

Digital Holographic Microscopy (DHM) is a technique that uses optical interference patterns to record a three-dimensional optical field for imaging, sensing, and microscopy applications. “Lensless” in-line DHM is the simplest arrangement, requiring no lenses, no mirrors, and typically only a light source, sample, and a digital imager chip such as a CCD or CMOS pixel array. Despite this simplicity, lensless in-line DHM is capable of producing high-resolution images over a wide field of view and allows researchers to record the amplitude and phase of a light field, and to digitally reconstruct the shape, thickness, 3D position, velocity, refractive index, and other parameters of cells or small particles. There are therefore many potential opportunities for combining in-line DHM with microfluidics , optical flow velocimetry, low-cost imaging, point-of-care diagnostics, single cell tracking, cell cytometry, counting, sorting, and lab-on-a-chip technologies.

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Authors and Affiliations

  1. Physics Department, Bethel University, St. Paul, MN, 55112, USA

    Nathan C. Lindquist

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  1. Nathan C. Lindquist
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Correspondence to Nathan C. Lindquist .

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Editors and Affiliations

  1. Department of Electrical and Computer Engineering, University of Minnesota, Twin Cities, Minnesota, USA

    Sang-Hyun Oh

  2. Department of Chemical Engineering, Queens University, Kingston, Ontario, Canada

    Carlos Escobedo

  3. Department of Chemistry, University of Victoria, Victoria, British Columbia, Canada

    Alexandre G. Brolo

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Lindquist, N.C. (2018). An Introduction to Lensless Digital Holographic Microscopy. In: Oh, SH., Escobedo, C., Brolo, A. (eds) Miniature Fluidic Devices for Rapid Biological Detection. Integrated Analytical Systems. Springer, Cham. https://doi.org/10.1007/978-3-319-64747-0_6

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