Abstract
The fast growing microalgae Chlorella vulgaris were isolated from fresh water bodies Pallikaranai at Chennai, Tamil Nadu, India and the species were focused for high lipid extraction for biodiesel production. The Optical Microscope and Field Emission Scanning Electron Microscope studies were performed to examine the morphology of Chlorella vulgaris. The Quantitative analysis of Intracellular components of Chlorella vulgaris were also examined by performing Nile red staining and observed under Epifluorescence Microscope. Various methods including Bligh and Dyer, Modified Bligh and Dyer method, Ultrasonication, Bead Vortexing, Shake Mill, Water Bath and Osmotic Shock methods were experimented to identify the effective cell disruption of Chlorella vulgaris for high yield of lipid content. Among which the Osmotic shock method was found to be an effective method for the cell disruption of Chlorella vulgaris which gives a yield of 26.1%. Therefore the extracted lipids were subjected to GC—MS for the analysis of fatty acid composition which is responsible for biodiesel production. Fatty acid methyl esters like C14:0, C16:0, C17:0, C18:1 are found to be present in the lipid content of Chlorella vulgaris which ensures its capabilities for biodiesel production.
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Acknowledgements
The authors wish to acknowledge the Management of Sathyabama University- Dean (Research and Development), and The Ministry of Human Resource and Development (Sanctioned no: 5-5/2014-TS. VII 4th September 2014), Government of India for their consistent encouragement and financial support for the Research activities.
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Nirmala, N., Dawn, S.S., Rengith, S.S. (2018). Qualitative and Quantitative Analysis of Lipid Derived via Cell Disruption Techniques from Chlorella vulgaris for Biodiesel Production. In: Anand, G., Pandey, J., Rana, S. (eds) Nanotechnology for Energy and Water . ICNEW 2017. Springer Proceedings in Energy. Springer, Cham. https://doi.org/10.1007/978-3-319-63085-4_18
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DOI: https://doi.org/10.1007/978-3-319-63085-4_18
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