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Vitiligo pp 313-328 | Cite as

Regenerating Melanocytes: Current Stem Cell Approaches with Focus on Muse Cells

  • Mari DezawaEmail author
  • Kenichiro Tsuchiyama
  • Kenshi Yamazaki
  • Setsuya Aiba
Chapter

Abstract

Muse cells are recently found endogenous non-tumorigenic pluripotent stem cells that reside in connective tissue of various organs including the dermis and in the bone marrow. They are collectable as cells positive for stage-specific embryonic antigen (SSEA)-3, a pluripotent surface marker, from tissues, and are expandable in vitro. Other than SSEA-3, they express Oct3/4, Nanog and Sox, other pluripotent genes. Notably, they are able to differentiate into cells representative of all three germ layers from single cells and are self-renewable, suggesting their pluripotency. Muse cells collected from human dermal fibroblasts (dermal-Muse cells) were shown to efficiently differentiate into melanin-producing functional melanocytes by treating them with ten factors. Functions of melanocytes induced from Muse cells (Muse melanocytes) were comparable to that of primary human melanocytes. Melanin-producing ability of human Muse melanocytes was retained when they were incorporated into human-colored three-dimensional (3D) cultured skin and even after transplantation of the 3D-cultured skin into the back of immunodeficient mice. Since Muse cells are non-tumorigenic and harvestable from easy accessible sources such as skin biopsy and dermal fibroblasts, Muse melanocytes are beneficial for both industrial and clinical uses.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Mari Dezawa
    • 1
    Email author
  • Kenichiro Tsuchiyama
    • 2
  • Kenshi Yamazaki
    • 2
  • Setsuya Aiba
    • 2
  1. 1.Department of Stem Cell Biology and HistologyTohoku University Graduate School of MedicineSendaiJapan
  2. 2.Department of DermatologyTohoku University Graduate School of MedicineSendaiJapan

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