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Vitiligo pp 225-236 | Cite as

In Vitro Study of Vitiligo

  • Maria Lucia Dell’AnnaEmail author
  • Muriel Cario-André
Chapter

Abstract

The study of vitiligo has been approached by several different perspectives. Accordingly to the progressive improvement of the technological support to the research and discovery of new fields in the biological world, even researchers who focused on vitiligo gained new opportunities. An unsolved question regards how and where to analyze the possible pathomechanisms underlying the vitiligo onset and progression. A clear-cut position against the best in vitro approach is aleatory. It is surely customary to use the primary cell cultures to perform functional studies; however, the in vitro assays with primary cell cultures are indissolubly affected by the intrinsic selection of the more “aggressive” or best cells among the overall tissue bulk. Moreover, the media usually used to select and grow the primary cells are optimized to burst their proliferative or differentiative ability, probably overcoming the intrinsic features of the isolated cells. The first requirement of a researcher is to maximize yield of a bioptic sample, but this requirement affects per se the quality of the resulting cultures that may no longer reflect the initial cellular asset of an individual. This initial reflection did not intend to attack the studies currently performed with primary cell cultures, but it just aims to alert about the possible intrinsic bias.

References

  1. 1.
    Medrano EE, Nordlund JJ. Successful culture of adult human melanocytes obtained from normal and vitiligo donors. J Invest Dermatol. 1990;95:441–5.Google Scholar
  2. 2.
    Kauser S, Thody AJ, Schallreuter KU, et al. β-Endorphin as a regulator of human hair follicle melanocyte biology. J Invest Dermatol. 2004;123:184–95.CrossRefGoogle Scholar
  3. 3.
    Tobin DJ, Colen SR, Bystryn JC. Isolation and long term culture of human hair-follicle melanocytes. J Invest Dermatol. 1995;104:86–9.CrossRefGoogle Scholar
  4. 4.
    Halaban R, Alfano FD. Selective elimination of fibroblasts from cultures of normal human melanocytes. In Vitro. 1984;20:447–50.CrossRefGoogle Scholar
  5. 5.
    Na GY, Paek SH, Park BC, et al. Isolation and characterization of outer root sheath melanocytes of human hair follicles. Br J Dermatol. 2006;155:902–9.CrossRefGoogle Scholar
  6. 6.
    Vennegor C, Hageman P, Van Nouhuijs H, et al. A monoclonal antibody specific for cells of the melanocyte lineage. Am J Pathol. 1988;130:179–92.Google Scholar
  7. 7.
    Ricard AS, Pain C, Daubos A, et al. Study of CCN3 (NOV) and DDR1 in normal melanocytes and vitiligo skin. Exp Dermatol. 2012;21:411–6.CrossRefGoogle Scholar
  8. 8.
    Wagner RY, Luciani F, Cario-André M, et al. Altered E-cadherin levels and distribution in melanocytes precede clinical manifestations of vitiligo. J Invest Dermatol. 2015;135:1810–9.CrossRefGoogle Scholar
  9. 9.
    Caliari SR, Burdick JA. A practical guide to hydrogels for cell culture. Nat Methods. 2016;13:405.CrossRefGoogle Scholar
  10. 10.
    Dell’Anna ML, Maresca V, Briganti S, et al. Mitochondrial impairment in peripheral blood mononuclear cells during the active phase of vitiligo. J Invest Dermatol. 2001;117:908–13.CrossRefGoogle Scholar
  11. 11.
    Donmez-altuntas H, Sut Z, Ferahbas A, et al. Increased micronucleus frequency in phytohaemagglutinin-stimulated blood cells of patients with vitiligo. J Eur Acad Dermatol Venereol. 2008;22:162–7.PubMedGoogle Scholar
  12. 12.
    Giovannelli L, Bellandi S, Pitozzi V, et al. Increased oxidative DNA damage in mononuclear leukocytes in vitiligo. Mutat Res. 2004;556:101–6.CrossRefGoogle Scholar
  13. 13.
    Prunieras M, Regnier M, Schlotterer M. [New procedure for culturing human epidermal cells on allogenic or xenogenic skin: preparation of recombined grafts]. Ann Chir Plast. 1979;24:357–362.Google Scholar
  14. 14.
    Poumay Y, Coquette A. Modelling the human epidermis in vitro: tools for the basic and applied research. Arch Dermatol Res. 2007;298:361–9.CrossRefGoogle Scholar
  15. 15.
    Bell E, Sher S, Hull B, et al. The reconstitution of living skin. J Invest Dermatol. 1983;81:2s–10s.CrossRefGoogle Scholar
  16. 16.
    Black AF, Bouez C, Perrier E, et al. Optimization and characterization of an engineered human skin equivalent. Tissue Eng. 2005;11:723–33.CrossRefGoogle Scholar
  17. 17.
    Lee DY, Lee JH, Yang JM, et al. A new dermal equivalent: the use of dermal fibroblast culture alone without exogenous materials. J Dermatol Sci. 2006;43:95–104.CrossRefGoogle Scholar
  18. 18.
    Rosdy M, Bjorklund MG, Asplud A, et al. Terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the hair-liquid interface. J Invest Dermatol. 1990;95:409–14.CrossRefGoogle Scholar
  19. 19.
    Jimbow K, Chen H, Park JS, et al. Increased sensitivity of melanocytes to oxidative stress and abnormal expression of tyrosinase-related protein in vitiligo. Br J Dermatol. 2001;144:55–65.CrossRefGoogle Scholar
  20. 20.
    Régnier M, Staquet MJ, Schmitt D, et al. Integration of Langerhans cells into a pigmented reconstructed human epidermis. J Invest Dermatol. 1997;109:510–2.CrossRefGoogle Scholar
  21. 21.
    Cario-André M, Bessou S, Gontier E, et al. The reconstructed epidermis with melanocytes: a new tool to study pigmentation and photo protection. Cell Mol Biol. 1999;45:931–42. Review (Erratum in: Cell MolBiol (2000); 446:489).PubMedGoogle Scholar
  22. 22.
    Bessou S, Surlève-Bazeille JE, Sorbier E, Taïeb A. Ex vivo reconstruction of the epidermis with melanocytes and the influence of UVB. Pigment Cell Res. 1995;8:241–9.CrossRefGoogle Scholar
  23. 23.
    Cario-André M, Pain C, Gaythier Y, et al. The melanocythorragic hypothesis of vitiligo tested on pigmented, stressed, reconstructed epidermis. Pigment Cell Res. 2007;20:385–93.PubMedGoogle Scholar
  24. 24.
    Dell’Anna ML, Ottaviani M, Albanesi V, et al. Membrane lipid alterations as a possible basis for melanocyte degeneration in vitiligo. J Invest Dermatol. 2007;127:1226–33.CrossRefGoogle Scholar
  25. 25.
    Ivanova K, van der Wijngaard R, Gerzer R, et al. Nonlesional vitiliginous melanocytes are not characterized by an increased proneness to nitric oxide-induced apoptosis. Exp Dermatol. 2005;14:445–53.CrossRefGoogle Scholar
  26. 26.
    Kroll TM, Bommiasamy H, Boissy RE, et al. 4-tertiary butylphenol exposure sensitizes human melanocytes to dendritic cell-mediated killing: relevance to vitiligo. J Invest Dermatol. 2005;124:798–806.CrossRefGoogle Scholar
  27. 27.
    Lee AY, Kim NH, Choi WI, et al. Less keratinocyte derived factors related to more keratinocyte apoptosis in depigmented than normally pigmented suction-blistered epidermis may cause passive melanocyte death in vitiligo. J Invest Dermatol. 2005;124:976–83.CrossRefGoogle Scholar
  28. 28.
    Maresca V, Roccella M, Roccella F, et al. Increased sensitivity to peroxidative agents as a possible pathogenetic factor of melanocyte damage in vitiligo. J Invest Dermatol. 1997;109:310–3.CrossRefGoogle Scholar
  29. 29.
    Yang F, Boissy RE. Effects of 4-tertiary butylphenol on the tyrosinase activity in human melanocytes. Pigment Cell Res. 1999;12:237–45.CrossRefGoogle Scholar
  30. 30.
    Yang F, Sarangarajan R, Le Poole IC, et al. The cytotoxicity and apoptosis induced by 4-tertiary butylphenol inhuman melanocytes are independent of tyrosinase activity. J Invest Dermatol. 2000;114:157–64.CrossRefGoogle Scholar
  31. 31.
    Zhang RZ, Zhu WY, Xia MY, et al. Morphology of cultured human epidermal melanocytes observed by atomic force microscopy. Pigment Cell Res. 2004;17:62–5.CrossRefGoogle Scholar
  32. 32.
    Boissy RE, Liu YY, Medrano EE, et al. Structural aberration of the rough endoplasmic reticulum and melanosome compartmentalization in long-term cultures of melanocytes from vitiligo patients. J Invest Dermatol. 1991;97:395–404.CrossRefGoogle Scholar
  33. 33.
    Bondanza S, Maurelli R, Paterna P, et al. Keratinocytes cultures from involved skin in vitiligo patients show an impaired in vitro behaviour. Pigment Cell Res. 2007;20:288–300.CrossRefGoogle Scholar
  34. 34.
    Van den Wijngaard RMJGJ, Aten J, Scheepmaker A, et al. Expression and modulation of apoptosis regulatory molecules in human melanocytes: significance in vitiligo. Br J Dermatol. 2000;143:573–81.CrossRefGoogle Scholar
  35. 35.
    Kungolovaski G, Jeltsch A. Epigenome editing: state of the art, concepts, and perspectives. Trends Genet. 2016;32:101–13.CrossRefGoogle Scholar
  36. 36.
    Clark SJ, Lee HL, Smallwood SA, Kelsey G, Reik W. Single-cell epigenomics: powerful new methods for understanding gene regulation and cell identity. Genome Biol. 2016;17:72–81.CrossRefGoogle Scholar
  37. 37.
    Eves PC, Beck AJ, Shard AG, et al. A chemically defined surface for the co-culture of melanocytes and keratinocytes. Biomaterials. 2005;26:7068–81.CrossRefGoogle Scholar
  38. 38.
    Graham A, Westerhof W, Thody AJ. The expression of a-MSH by melanocytes is reduced in vitiligo. Ann N Y Acad Sci. 1999;885:470–3.CrossRefGoogle Scholar
  39. 39.
    Kitamura R, Tsukamoto K, Harada K, et al. Mechanisms underlying the dysfunction of melanocytes in vitiligo epidermis: role of SCF/KIT protein interactions and the downstream effector, MITF-M. J Pathol. 2004;202:463–75.CrossRefGoogle Scholar
  40. 40.
    Norris A, Todd C, Graham A, et al. The expression of the c-kit receptor by epidermal melanocytes maybe reduced in vitiligo. Br J Dermatol. 1996;134:299–306.CrossRefGoogle Scholar
  41. 41.
    Dell’Anna ML, Urbanelli S, Mastrofrancesco A, et al. Alterations of mitochondria in peripheral blood mononuclear cells of vitiligo patients. Pigment Cell Res. 2003;16:553–9.CrossRefGoogle Scholar
  42. 42.
    Dykens JA, Fleck B, Ghosh S, et al. High-throughput assessment of mitochondrial membrane potential in situ using fluorescence resonance energy transfer. Mitochondrion. 2002;1:461–73.CrossRefGoogle Scholar
  43. 43.
    Tellez CS, Davis DW, Prieto VG, et al. Quantitative analysis of melanocytic tissue array reveals inverse correlation between activator protein-2 alpha and protease-activated receptor-1 expression during melanoma progression. J Invest Dermatol. 2007;127:387–93.CrossRefGoogle Scholar
  44. 44.
    Ouvry-Patat SA, Torres MP, Quek HH, et al. Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry. Proteomics. 2008;8:2798–808.CrossRefGoogle Scholar
  45. 45.
    Kawase A, Kushimoto T, Kawa Y, et al. Proteomic analysis of immature murine melanocytes at different stages of maturation: a crucial role for calreticulin. J Dermatol Sci. 2008;49:43–52.CrossRefGoogle Scholar
  46. 46.
    Gianazza E, Miller I, Palazzolo L, Parravicini C, Eberini I. With or without you-proteomics with or without major plasma/serum proteins. J Proteomics. 2016;140:62–80.CrossRefGoogle Scholar
  47. 47.
    O’Rourke MB, Padula MP. Analysis of formalin-fixed paraffin-embedded (FPPE) tissue via proteomic techniques and misconceptions of antigen retrieval. Biotechniques. 2016;60:229–38.PubMedGoogle Scholar
  48. 48.
    Cambiaghi A, Ferrario M, Masseroli M. Analysis of metabolomic data: tools, current strategies and future challenges for omics data integration. Brief Bioinform. 2017;18:498–510.PubMedGoogle Scholar
  49. 49.
    Han X. Potential mechanisms contributing to sulfatide depletion at the earliest clinically recognizable stage of Alzheimer’s disease: a tale of shotgun lipidomics. J Neurochem. 2007;103:171–9.CrossRefGoogle Scholar
  50. 50.
    Stromberg S, Bjorklund MG, Asplud A, et al. Transcriptional profiling of melanocytes from patients with vitiligo vulgaris. Pigment Cell Melanoma Res. 2008;21:162–71.CrossRefGoogle Scholar
  51. 51.
    Goding CR. Melanocytes: the new black. Pigment Cell Res. 2007;39:275–9.Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Laboratory of Cutaneous Physiopathology, San Gallicano Dermatological InstituteIFORomeItaly
  2. 2.Inserì U876, Centre de référence des maladies rares de la peauUniversité V Segalen Bordeaux 2BordeauxFrance

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