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Precision Molecular Pathology of Myeloid Neoplasms pp 369–412Cite as

Existing and Emerging Molecular Technologies in Myeloid Neoplasms

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Part of the book series: Molecular Pathology Library ((MPLB,volume 12))

Abstract

Insight into the genetic aberrations of myeloid neoplasms has been accomplished through the application of evolving technology and laboratory techniques. Techniques that have been employed for decades include karyotype and fluorescent in situ hybridization have been supplemented by methods such as polymerase chain reaction, Sanger sequencing, and deoxyribonucleic acid (DNA) microarrays that have provided additional insight into the genetic underpinnings of myeloid neoplasia. Recent advances in massively parallel sequencing permit unprecedented insight into the genetic abnormalities involved in the neoplastic process. New technologies, such as long-read sequencing technologies, are in development and offer the possibility of real-time sequencing of single molecules that could offer additional insights. Application of these techniques to detect minimal residual disease and single-cell sequencing is offering insight into the disease processes, but the integration of such methods into routine practice is currently unclear. Understanding the nuances of the analytical methods that are used to inform the genetic basis of myeloid neoplasia is critical for interpreting the results of clinical and scientific investigations.

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Authors and Affiliations

  1. Department of Laboratory Medicine, University of Washington, Seattle, WA, USA

    Eric Q. Konnick & David Wu

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  1. Eric Q. Konnick
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  2. David Wu
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Correspondence to Eric Q. Konnick .

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  1. Department of Pathology, Florida Hospital, University of Central Florida, Orlando, Florida, USA

    Chung-Che (Jeff) Chang

  2. Department of Pathology, Stanford University, Stanford, California, USA

    Robert S. Ohgami

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Konnick, E.Q., Wu, D. (2018). Existing and Emerging Molecular Technologies in Myeloid Neoplasms. In: Chang, CC., Ohgami, R. (eds) Precision Molecular Pathology of Myeloid Neoplasms. Molecular Pathology Library, vol 12. Springer, Cham. https://doi.org/10.1007/978-3-319-62146-3_19

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  • DOI: https://doi.org/10.1007/978-3-319-62146-3_19

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