Modelling Giant Lipid Vesicles Designed for Light Energy Transduction

  • Emiliano Altamura
  • Francesco Milano
  • Massimo Trotta
  • Pasquale Stano
  • Fabio MavelliEmail author
Part of the Lecture Notes in Bioengineering book series (LNBE)


In this paper a deterministic kinetic model describing giant lipid vesicles designed for the transduction of light into chemical energy will be presented and discussed. Although the model is based on a simplified mechanism, kinetic constants taken from experimental measurements have been used. The obtained results have shown that giant vesicles encapsulating the Reaction Center in the lipid membrane can exhibit, in suitable experimental conditions, a sensible increase of the internal pH in half an hour under a constant light irradiation.


Reaction center Giant vesicles Light transduction Kinetic model pH gradient 


  1. 1.
    Noireaux, V., Libchaber, A.: Proc. Natl. Acad. Sci. U.S.A. 101, 17669–17674 (2004)CrossRefGoogle Scholar
  2. 2.
    Luisi, P.L., Ferri, F., Stano, P.: Naturwissenschaften 93, 1–13 (2006)CrossRefGoogle Scholar
  3. 3.
    Morowitz, H.J., Heinz, B., Deamer, D.W.: Orig. Life Evol. Biosph. 18, 281–287 (1998)CrossRefGoogle Scholar
  4. 4.
    Szostak, J. W., Bartel, D. P., Luisi, P. L., Nature, 409, 387–39 (2001)Google Scholar
  5. 5.
    de Lorenzo, V., Danchin, A.: EMBO Rep. 9, 822–827 (2008)CrossRefGoogle Scholar
  6. 6.
    Pohorille, A., Deamer, D.: Trends Biotechnol. 20, 123–128 (2002)CrossRefGoogle Scholar
  7. 7.
    Küchler, A., Yoshimoto, M., Luginbühl, S., Mavelli, F., Walde, P.: Nat. Nanotech. 11, 409–420 (2016)CrossRefGoogle Scholar
  8. 8.
    Allen, J.P., Feher, G., Yeates, T.O., Komiya, H., Rees, D.C.: Proc. Natl. Acad. Sci. U.S.A. 85, 8487–8491 (1988)CrossRefGoogle Scholar
  9. 9.
    Allen, J.P., Williams, J.C.: FEBS Lett. 438, 5–9 (1988)CrossRefGoogle Scholar
  10. 10.
    Nagy, L., Milano, F., Dorogi, M., Trotta, M., Laczkó, G., Szebényi, K., Váró, G., Agostiano, A., Maróti, P.: Biochemistry 43, 12913–12923 (2004)CrossRefGoogle Scholar
  11. 11.
    Milano, F., Italiano, F., Agostiano, A., Trotta, M.: Photosynth. Res. 100, 107–112 (2009)CrossRefGoogle Scholar
  12. 12.
    Mavelli, F., Trotta, M., Ciriaco, F., Agostiano, A., Giotta, L., Italiano, F., Milano, F.: Eur Biophys J Biophys Lett 43, 301–315 (2014)CrossRefGoogle Scholar
  13. 13.
    Ollivon, M., Lesieur, S., Grabielle-Madelmont, C., Paternostre, M.: Biochim. Biophys. Acta 1508, 34–50 (2000)CrossRefGoogle Scholar
  14. 14.
    Stano, P., Carrara, P., Kuruma, Y., Souza, T.P., Luisi, P.L.: J. Mater. Chem. 21, 18887–18902 (2011)CrossRefGoogle Scholar
  15. 15.
    Pautot, S., Frisken, B.J., Weitz, D.A.: Proc. Natl. Acad. Sci. U.S.A. 100, 10718–10721 (2003)CrossRefGoogle Scholar
  16. 16.
    Grotzky, A., Altamura, E., Adamcik, J., Carrara, P., Stano, P., Mavelli, F., Nauser, T., Mezzenga, R., Schluter, A.D., Walde, P.: Langmuir 29, 10831–10840 (2013)CrossRefGoogle Scholar
  17. 17.
    Stano, P., Carrara, P., Kuruma, Y., Souza, T.P., Luisi, P.L.: J. Mater. Chem. 21, 18887–18902 (2011)CrossRefGoogle Scholar
  18. 18.
    Altamura, E., Stano, P., Walde, P., Mavelli, F.: Int. J. Unconv. Comp. 11, 5–21 (2015)Google Scholar
  19. 19.
    Mavelli, F.: BMC Bioinf 13(4), S10 (2012)CrossRefGoogle Scholar
  20. 20.
    Mavelli, F., Ruiz-Mirazo, K.: Integr Biol 5, 324–341 (2013)CrossRefGoogle Scholar
  21. 21.
    Mavelli, F., Altamura, E., Cassidei, L., Stano, P.: Entropy 16, 2488–2511 (2014)CrossRefGoogle Scholar
  22. 22.
    Mavelli, F., Stano, P.: Artif Life 21, 1–19 (2015)CrossRefGoogle Scholar
  23. 23.
    Geyer, T., Lauck, F., Helms, V.: J. Biotech. 129, 212–228 (2007)CrossRefGoogle Scholar
  24. 24.
    Geyer, T., Lauck, F., Mol, X., Blaß, S., Helms, V.: PLoS ONE 5, e14070 (2010)CrossRefGoogle Scholar
  25. 25.
    Mavelli, F., Piotto, S.: J. Mol. Struct. 771, 55–64 (2006)CrossRefGoogle Scholar
  26. 26.
    Vekshin, N.: Photonics of biopolymers. Springer-Verlag, Berlin Heidelberg (2002)CrossRefGoogle Scholar
  27. 27.
    Sener, M.K., Olsen, J.D., Hunter, C.N., Schulten, K.: Proc. Natl. Acad. Sci. U.S.A. 104, 15723–15728 (2007)CrossRefGoogle Scholar

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© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.Dipartimento Di ChimicaUniversità “Aldo Moro” Bari-ItalyBariItaly
  2. 2.CNR-IPCF, Istituto Per I Processi Chimico FisiciBariItaly
  3. 3.Science DepartmentRoma Tre UniversityRomeItaly

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