Skip to main content
SpringerLink
Log in

Production of Nanostructured Microspheres Biopolymer-Active Principle-Magnetic Nanoparticles by Supercritical Assisted Atomization

  • Chapter
  • First Online:
Advances in Bionanomaterials

Part of the book series: Lecture Notes in Bioengineering ((LNBE))

  • 675 Accesses

Abstract

Supercritical Assisted Atomization (SAA) has been applied to the production of nanostructured microspheres ampicillin-chitosan-magnetic nanoparticles (AMP-CH-NMPs). Several ampicillin/chitosan (AMP/CH) ratios with a fixed content of NMPs were processed in acid water solutions, to produce microspheres with different size, drug content and amount of NMPs. To verify the successful formation of microparticles, drug content and nanoparticle dispersion, they were characterized by SEM (Scanning Electron Microscope), EDX (Energy Dispersive X-ray), TGA (ThermoGravimetric Analysis), HPLC (High Performance Liquid Chromatography), UV-vis obtaining information on morphology, particle size distribution, nanostructure, loading of active principle in the polymeric matrix and drug release rate. Spherical microparticles were obtained, with a maximum particle size of 2 µm and loading efficiencies up to 99%. The microspheres produced by SAA showed a controlled release of the drug over about 72 h.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Felinto, M.C.F.C., Camilo, R.L., Diegues, T.G.: Magnetic nanoparticles and their application in biomedicine. In: International Nuclear Atlantic Conference—INAC 2007, Satnos, SP, Brazil (2007)

    Google Scholar 

  2. Neuberger, T., Schopf, B., Hofmann, H., Hofmann, M., von Rechenberg, B.: Superparamagnetic nanoparticles for biomedical applications: possibilities and limitations of a new drug delivery system. J. Magn. Magn. Mater. 293, 483–496 (2005)

    Article  Google Scholar 

  3. Chomoucka, J., Drbohlavova, J., Huska, D., Adam, V., Kizek, R., Hubalek, J.: Magnetic nanoparticles and targeted drug delivering. Pharmacol. Res. 62, 144–149 (2010)

    Article  Google Scholar 

  4. Pinto-Alphandary, H., Andremont, A., Couvreur, P.: Targeted delivery of antibiotics using liposomes and nanoparticles: research and applications. Int. J. Antimicrob. Ag. 13, 155–168 (2000)

    Article  Google Scholar 

  5. Faraji, A.H., Wipf, P.: Nanoparticles in cellular drug delivery. Bioorgan. Med. Chem. 17, 2950–2962 (2009)

    Article  Google Scholar 

  6. Chandy, T., Sharma, C.P.: Chitosan matrix for oral sustained delivery of ampicillin. Biomaterials 14, 939–944 (1993)

    Article  Google Scholar 

  7. Saha, P., Goyal, A.K., Rath, G.: Formulation and evaluation of chitosan-based ampicillin trihydrate nanoparticles. Tropical J. Pharm. Res. 9, 483–488 (2010)

    Article  Google Scholar 

  8. Changerath, R., Nalr, P.D., Mathew, S., Fteghunadhan, C.P.: Nalr: Poly(methyl methacrylate)-grafted chitosan microspheres for controlled release of ampicillin. J. Biomed. Mater. Res.—Part B Appl. Biomater. 89, 65–76 (2009)

    Article  Google Scholar 

  9. Ball, A.P., Bartlett, J.G., Craig, W.A., Drusano, G.L., Felmingham, D., Garau, J.A., Klugman, K.P., Low, D.E., Mandell, L.A., Rubinstein, E., Tillotson, G.S.: Future trends in antimicrobial chemotherapy: expert opinion on the 43rd ICAAC. J. Chemother. 16, 419–436 (2004)

    Article  Google Scholar 

  10. Huh, A.J., Kwon, Y.J.: “Nanoantibiotics”: a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. J. Control Release 156, 128–145 (2011)

    Article  Google Scholar 

  11. Hussein-Al-Ali, S.H., El Zowalaty, M.E., Hussein, M.Z., Geilich, B.M., Webster, T.J.: Synthesis, characterization, and antimicrobial activity of an ampicillin-conjugated magnetic nanoantibiotic for medical applications. Int. J. Nanomed. 9, 3801–3814 (2014)

    Article  Google Scholar 

  12. Baldino, L., Cardea, S., Reverchon, E.: Production of antimicrobial membranes loaded with potassium sorbate using a supercritical phase separation process. Innov. Food Sci. Emerg. 34, 77–85 (2016)

    Article  Google Scholar 

  13. Reverchon, E., Adami, R.: Nanomaterials and supercritical fluids. J. Supercrit. Fluid 37, 1–22 (2006)

    Article  Google Scholar 

  14. Reverchon, E., Sesti Osseo, L., Gorgoglione, D.: Supercritical CO2 extraction of basil oil: characterization of products and process modeling. J. Supercrit. Fluids 7, 185–190 (1994)

    Article  Google Scholar 

  15. Rossmann, M., Braeuer, A., Schluecker, E.: Supercritical antisolvent micronization of PVP and ibuprofen sodium towards tailored solid dispersions. J. Supercrit. Fluids 89, 16–27 (2014)

    Article  Google Scholar 

  16. De Paz, E., Martín, Á., Every, H., Cocero, M.J.: Production of water-soluble quercetin formulations by antisolvent precipitation and supercritical drying. J. Supercrit. Fluid 104, 281–290 (2015)

    Article  Google Scholar 

  17. Kurniawansyah, F., Mammucari, R., Foster, N.R.: Inhalable curcumin formulations by supercritical technology. Powder Technol. 284, 289–298 (2015)

    Article  Google Scholar 

  18. Santiago, L.M., Masmoudi, Y., Tarancón, A., Djerafi, R., Bagán, H., García, J.F., Badens, E.: Polystyrene based sub-micron scintillating particles produced by supercritical anti-solvent precipitation. J. Supercrit. Fluid 103, 18–27 (2015)

    Article  Google Scholar 

  19. Nerome, H., Machmudah, S., Wahyudiono, R., Fukuzato, T., Higashiura, H., Kanda, M.Goto: Effect of solvent on nanoparticle production of β-Carotene by a supercritical antisolvent process. Chem. Eng. Technol. 39, 1771–1777 (2016)

    Article  Google Scholar 

  20. Shen, Y.B., Du, Z., Wang, Q., Guan, Y.X., Yao, S.J.: Preparation of chitosan microparticles with diverse molecular weights using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer. Powder Technol. 254, 416–424 (2014)

    Article  Google Scholar 

  21. Wu, H.T., Yang, M.W., Huang, S.C.: Sub-micrometric polymer particles formation by a supercritical assisted-atomization process. J. Taiwan Inst. Chem. E. 45, 1992–2001 (2014)

    Article  Google Scholar 

  22. Prosapio, V., De Marco, I., Reverchon, E.: PVP/corticosteroid microspheres produced by supercritical antisolvent coprecipitation. Chem. Eng. J. 292, 264–275 (2016)

    Article  Google Scholar 

  23. Della Porta, G., Campardelli, R., Cricchio, V., Oliva, F., Maffulli, N., Reverchon, E.: Injectable PLGA/Hydroxyapatite/Chitosan microcapsules produced by supercritical emulsion extraction technology: an in vitro study on Teriparatide/Gentamicin controlled release. J. Pharm. Sci.-Us. 105, 2164–2172 (2016)

    Article  Google Scholar 

  24. Adami, R., Liparoti, S., Reverchon, E.: A new supercritical assisted atomization configuration, for the micronization of thermolabile compounds. Chem. Eng. J. 173, 55–61 (2011)

    Article  Google Scholar 

  25. Wu, H.T., Huang, S.C., Yang, C.P., Chien, L.J.: Precipitation parameters and the cytotoxicity of chitosan hydrochloride microparticles production by supercritical assisted atomization. J. Supercrit. Fluid 102, 123–132 (2015)

    Article  Google Scholar 

  26. Shen, Y.B., Guan, Y.X., Yao, S.J.: Supercritical fluid assisted production of micrometric powders of the labile trypsin and chitosan/trypsin composite microparticles. Int. J. Pharm. 489, 226–236 (2015)

    Article  Google Scholar 

  27. Labuschagne, P.W., Adami, R., Liparoti, S., Naidoo, S., Swai, H., Reverchon, E.: Preparation of rifampicin/poly(D, L-lactice) nanoparticles for sustained release by supercritical assisted atomization technique. J. Supercrit. Fluid 95, 106–117 (2014)

    Article  Google Scholar 

  28. Liparoti, S., Adami, R., Caputo, G., Reverchon, E.: Supercritical assisted atomization: polyvinylpyrrolidone as carrier for drugs with poor solubility in water. J. Chem.-Ny. (2013)

    Google Scholar 

  29. Adami, R., Liparoti, S., Della Porta, G., Del Gaudio, P., Reverchon, E.: Lincomycin hydrochloride loaded albumin microspheres for controlled drug release, produced by supercritical assisted atomization. J. Supercrit. Fluid 119, 203–210 (2017)

    Article  Google Scholar 

  30. Shen, Y.B., Du, Z., Tang, C., Guan, Y.X., Yao, S.J.: Formulation of insulin-loaded N-trimethyl chitosan microparticles with improved efficacy for inhalation by supercritical fluid assisted atomization. Int. J. Pharm. 505, 223–233 (2016)

    Article  Google Scholar 

  31. Wu, H.T., Yang, C.P., Huang, S.C.: Dissolution enhancement of indomethacin-chitosan hydrochloride composite particles produced using supercritical assisted atomization. J. Taiwan Inst. Chem. E. 67, 98–105 (2016)

    Article  Google Scholar 

  32. Adami, R., Reverchon, E.: Composite polymer-Fe3O4 microparticles for biomedical applications, produced by supercritical assisted atomization. Powder Technol. 218, 102–108 (2012)

    Article  Google Scholar 

  33. Reverchon, E., Adami, R.: Supercritical assisted atomization to produce nanostructured chitosan-hydroxyapatite microparticles for biomedical application. Powder Technol. 246, 441–447 (2013)

    Article  Google Scholar 

  34. Reverchon, E., Antonacci, A.: Drug-polymer microparticles produced by supercritical assisted atomization. Biotechnol. Bioeng. 97, 1626–1637 (2007)

    Article  Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge Dr. Valentina Gregori and Dr. Alessia Di Capua for the help in performing the experiments. The MiUR (Ministero dell’istruzione, dell’Università e della Ricerca) is acknowledged for the financial support.

Author information

Authors and Affiliations

  1. Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy

    Renata Adami, Mariarosa Scognamiglio & Ernesto Reverchon

Authors
  1. Renata Adami
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mariarosa Scognamiglio
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ernesto Reverchon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Renata Adami .

Editor information

Editors and Affiliations

  1. Department of Pharmacy, University of Salerno Department of Pharmacy, Fisciano, Italy

    Stefano Piotto

  2. Chemistry and Biology, University of Salerno Chemistry and Biology, Fisciano, Italy

    Federico Rossi

  3. Department of Industrial Engineering, University of Salerno Department of Industrial Engineering, Fisciano, Italy

    Simona Concilio

  4. Department of Industrial Engineering, University of Salerno Department of Industrial Engineering, Fisciano, Italy

    Ernesto Reverchon

  5. Department of Informatics, University of Salerno Department of Informatics, Fisciano, Italy

    Giuseppe Cattaneo

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG

About this chapter

Cite this chapter

Adami, R., Scognamiglio, M., Reverchon, E. (2018). Production of Nanostructured Microspheres Biopolymer-Active Principle-Magnetic Nanoparticles by Supercritical Assisted Atomization. In: Piotto, S., Rossi, F., Concilio, S., Reverchon, E., Cattaneo, G. (eds) Advances in Bionanomaterials. Lecture Notes in Bioengineering. Springer, Cham. https://doi.org/10.1007/978-3-319-62027-5_2

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-62027-5_2

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-62026-8

  • Online ISBN: 978-3-319-62027-5

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics

Search

Navigation