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Nanoliposomes Production by a Protocol Based on a Simil-Microfluidic Approach

  • Sabrina Bochicchio
  • Annalisa Dalmoro
  • Federica Recupido
  • Gaetano Lamberti
  • Anna Angela BarbaEmail author
Chapter
Part of the Lecture Notes in Bioengineering book series (LNBE)

Abstract

In this work a protocol based on the microfluidic principles has been developed and applied to produce nanoliposomes. The protocol basically consists in the realization of a contact between two flows, lipids/ethanol and water solutions, inside a tubular device where interdiffusion phenomena allow the formation of lipid vesicles. Effects of solutions flow rates and lipids concentrations on size and size distribution have been investigated. Moreover, ultrasonic energy was used to enhance homogenization of the hydroalcoholic final solutions and to promote the vesicles size reduction. By this protocol a massive output has been achieved; increasing the ratio between the water volumetric flow rate to the lipids-ethanol volumetric flow rate the liposomes dimension decreases; at equal flow rates, when the lipids concentration increases also the liposomes size has been observed increasing.

keywords

Liposomes Ultrasonic energy Semicontinuous bench scale apparatus 

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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • Sabrina Bochicchio
    • 1
    • 2
  • Annalisa Dalmoro
    • 2
  • Federica Recupido
    • 1
    • 2
  • Gaetano Lamberti
    • 1
  • Anna Angela Barba
    • 2
    Email author
  1. 1.Department of Industrial EngineeringUniversity of SalernoFiscianoItaly
  2. 2.Department of PharmacyUniversity of SalernoFiscianoItaly

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