Nanoliposomes Production by a Protocol Based on a Simil-Microfluidic Approach

  • Sabrina Bochicchio
  • Annalisa Dalmoro
  • Federica Recupido
  • Gaetano Lamberti
  • Anna Angela BarbaEmail author
Part of the Lecture Notes in Bioengineering book series (LNBE)


In this work a protocol based on the microfluidic principles has been developed and applied to produce nanoliposomes. The protocol basically consists in the realization of a contact between two flows, lipids/ethanol and water solutions, inside a tubular device where interdiffusion phenomena allow the formation of lipid vesicles. Effects of solutions flow rates and lipids concentrations on size and size distribution have been investigated. Moreover, ultrasonic energy was used to enhance homogenization of the hydroalcoholic final solutions and to promote the vesicles size reduction. By this protocol a massive output has been achieved; increasing the ratio between the water volumetric flow rate to the lipids-ethanol volumetric flow rate the liposomes dimension decreases; at equal flow rates, when the lipids concentration increases also the liposomes size has been observed increasing.


Liposomes Ultrasonic energy Semicontinuous bench scale apparatus 


  1. 1.
    Attama, A.A., Momoh, M.A., Builders, P.F.: Lipid nanoparticulate drug delivery systems: a revolution in dosage form design and development, pp. 107–140. InTech, Croatia (2012)Google Scholar
  2. 2.
    Sawant, R.R., Torchilin, V.P.: Challenges in development of targeted liposomal therapeutics. The AAPS J. 14(2), 303–315 (2012)CrossRefGoogle Scholar
  3. 3.
    Reza Mozafari, M., et al.: Nanoliposomes and their applications in food nanotechnology. J. Liposome Res. 18(4), 309–327 (2008)CrossRefGoogle Scholar
  4. 4.
    Bochicchio, S., et al.: Vitamin delivery: carriers based on nanoliposomes produced via ultrasonic irradiation. LWT-Food Sci. Technol. 69, 9–16 (2016)CrossRefGoogle Scholar
  5. 5.
    Meure, L.A., Foster, N.R., Dehghani, F.: Conventional and dense gas techniques for the production of liposomes: a review. AAPS Pharmscitech 9(3), 798–809 (2008)CrossRefGoogle Scholar
  6. 6.
    Wagner, A., Vorauer-Uhl, K.: Liposome technology for industrial purposes. J Drug Deliv., 9 (2011)Google Scholar
  7. 7.
    Bangham, A., Horne, R.: Negative staining of phospholipids and their structural modification by surface-active agents as observed in the electron microscope. J. Mol. Bio. 8(5), 660-IN10 (1964)Google Scholar
  8. 8.
    Pradhan, P., et al.: A facile microfluidic method for production of liposomes. Anticancer Res. 28(2A), 943–947 (2008)MathSciNetGoogle Scholar
  9. 9.
    Barba, A., et al.: Ultrasonic energy in liposome production: process modelling and size calculation. Soft Matter 10(15), 2574–2581 (2014)CrossRefGoogle Scholar
  10. 10.
    Jahn, A., et al.: Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing. J. Am. Chem. Soc. 126(9), 2674–2675 (2004)CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • Sabrina Bochicchio
    • 1
    • 2
  • Annalisa Dalmoro
    • 2
  • Federica Recupido
    • 1
    • 2
  • Gaetano Lamberti
    • 1
  • Anna Angela Barba
    • 2
    Email author
  1. 1.Department of Industrial EngineeringUniversity of SalernoFiscianoItaly
  2. 2.Department of PharmacyUniversity of SalernoFiscianoItaly

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