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Steroid Profiles Throughout the Hormonal Stimulation in Females Siberian Sturgeon Acipenser baerii Brandt

  • Patrick Williot
  • Sylvain Comte
  • Françoise Le Menn
  • Blandine Davail-Cuisset
Chapter

Abstract

By setting up a cannulation on farmed Siberian sturgeon spawners, patterns of testosterone (T), 11-ketotestosterone (11-KT), 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) and oestradiol (E2) are established in non-stressed females throughout complete two alternatives of spawning procedures. Treatment 1 consisted in (a) setting up the cannula together with the ovarian follicles sample; (b) later on, injecting the fish with hormones without any handling; and (c) collecting the ovulated eggs. Treatment 2 contracted the setting up of the cannula, the sampling of the ovarian follicles and the hormonal injection in one handling; collection of ovulated eggs underwent later on. Oestradiol remained at a very low level from 0.3 to 1.2 ng/mL throughout the experiments. Figures for T show a surge 6–12 h post-hormonal injection from 150–210 ng/mL up to 260–320 ng/mL for treatment 1 and 2, respectively. Peaks of T declined rapidly in treatment 1 and last around 20 h in treatment 2 before declining to low initial values. 11-KT increased slowly postinjection in a lower extent than for T with a maximum 20–25 h post-hormonal injection with about 60 and 80 ng/mL for treatments 1 and 2, respectively. Figures for treatment 2 are pulsation-like. 17,20βP increased postinjection more rapidly in treatment 2 and decreased before ovulation. Altogether, treatment 2, characterised by a two-step spawning procedure instead of three in treatment 1, showed higher values and more durable elevated levels of steroids than for treatment 1. We discuss the above results in the light of sturgeon-related literature.

Keywords

Acipenser baerii Farming Cannulation Induced spawning Steroids profiles 

Notes

Acknowledgments

Authors are grateful to Drs. Zanui and Carillo for the gift of E2 antibody and to Dr. Fostier for the gifts of 17,20βP as well as 17,20βP radioactive tracer.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Patrick Williot
    • 1
    • 2
  • Sylvain Comte
    • 2
  • Françoise Le Menn
    • 3
  • Blandine Davail-Cuisset
    • 3
    • 4
  1. 1.AudengeFrance
  2. 2.Cemagref (Now Irstea)CestasFrance
  3. 3.Former Laboratory of Biology of the Reproduction of Fish, University of Bordeaux ITalence CedexFrance
  4. 4.Laboratory of Physical and Toxical Chemistry, Environmental Toxicology, University of Bordeaux 1Talence CedexFrance

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