Abstract
Chitin is a vital component of insect exoskeleton and peritrophic matrix and because of this reason a potential target for insecticidal agents. Chitin-metabolizing enzymes, viz., chitin synthases and chitinases, belong to the glycoside hydrolase superfamily (GH18). Chitin synthases are involved in deposition of new cuticle during molting and also ideal for development of insecticidal agents. Chitinases are considered as an essential enzyme for insect growth and development being involved in molting and various other physiological processes, i.e., cuticle turnover, regulation of abdominal contraction and wing expansion, digestion, immunity, and natural defense. Chitinases possess multi-domain architecture, i.e., chitin-binding domain, Ser-/Thr-rich linker domains, catalytic domains, fibronectin, and mucin-like domains. Knockdown of both the enzymes resulted into irregularities in metamorphosis. Diverse group of chitinase-like proteins have also been detected in insect species that possess chitin-binding domains but do not exhibit catalytic activity. Development of chitinases as defensive agents against chitin-bearing insect pests and pathogens will generate new knowledge and innovative processes for biocontrol advancements.
Keywords
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Abdel-Banat BMA, Kameyama Y, Yoshioka T, Koga D (1999) Purification and characterization of a 54 kDa chitinase from Bombyx mori. Insect Biochem Mol Biol 30:107–117
Abo-Elghar GE, Fujiyoshi P, Matsumura F (2004) Significance of the sulfonylurea receptor (SUR) as the target of diflubenzuron in chitin synthesis inhibition in Drosophila melanogaster and Blattella germanica. Insect Biochem Mol Biol 34:743–752
Ahmad T, Rajagopal R, Bhatnagar RK (2003) Molecular characterization of chitinase from polyphagous pest Helicoverpa armigera. Biochem Biophys Res Commun 310:188–195
Arakane Y, Muthukrishnan S (2010) Insect chitinase and chitinase-like proteins. Cell Mol Life Sci 67:201–216
Arakane Y, Zhu Q, Matsumiya M, Muthukrishnan S, Kramer KJ (2003) Properties of catalytic, linker and chitin-binding domains of insect chitinase. Insect Biochem Mol Biol 33:631–648
Arakane Y, Hogenkamp DG, Zhu YC et al (2004) Characterization of two chitin synthase genes of the red flour beetle, Tribolium castaneum, and alternate exon usage in one of the genes during development. Insect Biochem Mol Biol 34:291–304
Arakane Y, Muthukrishnan S, Kramer K et al (2005) The Tribolium chitin synthase genes TcCHS1 and TcCHS2 are specialized for synthesis of epidermal cuticle and midgut peritrophic matrix. Insect Mol Biol 14:453–463
Arakane Y, Specht CA, Kramer KJ et al (2008) Chitin synthases are required for survival, fecundity and egg hatch in the red flour beetle, Tribolium castaneum. Insect Biochem Mol Biol 38:959–962
Aronson NN, Halloran BA, Alexyev ME, Amable L, Madura JD (2003) Family 18 chitinase oligosaccharide substrate interaction: subsite preference and anomer selectivity of S. marcescens chitinase A. J Biochem 376:87–95
Bade ML (1974) Localisation of molting chitinase in insect cuticle. Biochim Biophys Acta 372:474–477
Bao W, Cao B, Zhang Y, Wuriyanghan H (2016) Silencing of Mythimna separata chitinase genes via oral delivery of in planta-expressed RNAi effectors from a recombinant plant virus. Biotechnol Lett 38:1961–1966. doi:10.1007/s10529-016-2186-0
Bortone K, Monzingo AE, Ernst S, Robertus JD (2002) The structure of an allosamidin complex with the Coccidioides immitis chitinase defines a role for a second acid residue in substrate-assisted mechanism. J Mol Biol 320:293–302
Brameld KA, Shrader WD, Imperiali B, Goddard WA (2002) Substrate assistance in the mechanism of family 18 chitinases: theoretical studies of potential intermediates and inhibitors. J Mol Biol 280:913–923
Chapman RF (1998) The insects: structure and function. Cambridge University Press, New York
Chen AC (1987) Chitin metabolism. Arch Insect Biochem Physiol 6:267–277
Chen L, Yang W-J, Cong L et al (2013) Molecular cloning, characterization and mRNA expression of a chitin synthase 2 gene from the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae). Int J Mol Sci 14:17055–17072
Chen L, Liu T, Zhou Y, Chen Q, Shen X, Yang Q (2014a) Structural characteristics of an insect group I chitinase, an enzyme indispensable to moulting. Acta Crystallogr D Biol Crystallogr 70:932–942
Chen L, Zhou Y, Qu M, Zhao Y, Yang Q (2014b) Fully deacetylated chitooligosaccharides act as efficient glycoside hydrolase family 18 chitinase inhibitors. J Biol Chem 289:17932–17940
Cohen E (1987) Chitin biochemistry: synthesis and inhibition. Annu Rev Entomol 32:71–93
Coutinho PM, Deleury E, Davies GJ et al (2003) An evolving hierarchical family classification for glycosyltransferases. J Mol Biol 328:307–317
De Cock A, Degheele D (1998) Buprofezin: a novel chitin synthesis inhibitor affecting specifically planthoppers, whiteflies and scale insects. In: Insecticides with novel modes of action. Springer, Berlin Heidelberg, pp 74–91
Demaeght P, Osborne EJ, Odman-Naresh J et al (2014) High resolution genetic mapping uncovers chitin synthase-1 as the target-site of the structurally diverse mite growth inhibitors clofentezine, hexythiazox and etoxazole in Tetranychus urticae. Insect Biochem Mol Biol 51:52–61
Dorfmueller HC, Ferenbach AT, Borodkin VS et al (2014) A structural and biochemical model of processive chitin synthesis. J Biol Chem 289:23020–23028
Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y (2005) Cloning of Beauveria bassiana chitinase gene bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol 71:363–370
Filho BPD, Lemos EA, Secundino NEC, Pascoa V, Pereira ST (2002) Presence of chitinase and β -N-acetylglucosaminidase in the Aedes aegypti chitinolytic system involving peritrophic matrix formation and degradation. Insect Biochem Mol Biol 32:1723–1729
Fitches E, Wilkinson H, Bell H, Bown DP, Gatehouse JA, Edwards JP (2004) Cloning, expression and functional characterisation of chitinase from larvae of tomato moth (Lacanobia oleracea): a demonstration of the insecticidal activity of insect chitinase. Insect Biochem Mol Biol 34:1037–1050
Fontoura NG, Bellinato DF, Valle D et al (2012) The efficacy of a chitin synthesis inhibitor against field populations of organophosphate-resistant Aedes aegypti in Brazil. Mem Inst Oswaldo Cruz 107:387–395
Fukamizo T (2000) Chitinolytic enzymes: catalysis, substrate binding, and their application. Curr Protein Pept Sci 1:105–124
Harris MT, Fuhrman JA (2002) Structure and expression of chitin synthase in the parasitic nematode Dirofilaria immitis. Mol Biochem Parasitol 122:231–234
Hogenkamp DG, Arakane Y, Zimoch L et al (2005) Chitin synthase genes in Manduca sexta: characterization of a gut-specific transcript and differential tissue expression of alternately spliced mRNAs during development. Insect Biochem Mol Biol 35:529–540
Honda Y, Kitaoka M, Tokuyasu K, Sasaki C, Fukamizo T et al (2003) Kinetic studies on the hydrolysis of N-acetylated and N-deacetylated derivatives of 4-methylumbelliferyl chitobioside by the family 18 chitinases ChiA and ChiB from Serratia marcescens. J Biochem (Tokyo) 133:253–258
Huang X, Zhang H, Zen KC, Muthukrishnan S, Kramer KJ (2000) Homology modeling of the insect chitinase catalytic domain–oligosaccharide complex and the role of a putative active site tryptophan in catalysis. Insect Biochem Mol Biol 30:107–117
Huang Z, Hao Y, Gao T, Huang Y, Ren S, Keyhani NO (2016) The Ifchit1 chitinase gene acts as a critical virulence factor in the insect pathogenic fungus Isaria fumosorosea. Appl Microbiol Biotechnol 100:5491–5503
Ibrahim GH, Smartt CT, Kiley LM et al (2000) Cloning and characterization of a chitin synthase cDNA from the mosquito Aedes aegypti. Insect Biochem Mol Biol 30:1213–1222
Kaya M, Tozak KÖ, Baran T et al (2013) Natural porous and nano fiber chitin structure from Gammarus argaeus (Gammaridae Crustacea). Excli J (In eCollection) 12:503–510
Kaya M, Lelesius E, Nagrockaite R, Sargin I, Arslan G, Mol A, Baran T, Can E, Bitim B (2015) Differentiations of chitin content and surface morphologies of chitins extracted from male and female Grasshopper species. PLoS One 10:1–14
Kaya M, Sargin I, Al-jafa I, Erdogan S, Arslan G (2016a) Characteristics of corneal lens chitin in dragonfly compound eyes. Int J Biol Macromol 89:54–61
Kaya M, Sofi K, Sargin I, Mujtabaa M (2016b) Changes in physicochemical properties of chitin at developmental stages (larvae, pupa and adult) of Vespa crabro (wasp). Carbohydr Polym 145:64–70
Kim MG, Shin SW, Bae KS, Kim SC, Park HY (1998) Molecular cloning of chitinase cDNAs from the silkworm, Bombyx mori and the fall webworm, Hyphantria cunea. Insect Biochem Mol Biol 28:163–171
Koga D, Funakoshi T, Mizuki K, Ide A, Kramer KJ, Zen KC, Choi H, Muthukrishnan S (1992) Immunoblot analysis of chitinolytic enzymes in integument and molting fluid of the silkworm Bombyx mori and the tobacco hornworm Manduca sexta. Insect Biochem Mol Biol 22:305–311
Kola VSR, Renuka P, Madhav MS et al (2015) Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing. Front Physiol 6:119–134
Kramer KJ, Koga D (1986) Insect chitin: physical state, synthesis, degradation and metabolic regulation. Insect Biochem 16:851–877
Kramer KJ, Muthukrishnan S (1997) Insect chitinases: molecular biology and potential use as biopesticides. Insect Biochem Mol Biol 27:897–900
Kramer KJ, Muthukrishnan S (2005) Chitin metabolism in insects. In: Gilbert LI, Iatrou K, Gill S (eds) Comprehensive molecular insect science, vol 4. Elsevier, Oxford, pp 111–144
Kramer K, Muthukrishnan S (2009) Chitin metabolism in insects. In: Gillbert LI (ed) Insect development: morphogenesis, molting and metamorphosis. Academic Press, London, pp 497–530
Kramer KJ, Corpuz LM, Choi H, Muthukrishnan S (1993) Sequence of a eDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta. Insect Biochem Mol Biol 23:691–701
Kucerova L, Broz V, Arefin B, Maaroufi HO, Hurychova J, Strnad H, Zurovec M, Theopold U (2016) The Drosophila chitinase-like protein IDGF3 is involved in protection against nematodes and in wound healing. J Innate Immun 8:199–210
Li D, Zhang J, Wang Y, Liu X, Ma E, Sun Y, Li S, Zhu KY, Zhang J (2015a) Two chitinase 5 genes from Locusta migratoria: molecular characteristics and functional differentiation. Insect Biochem Mol Biol 58:46–54
Li X, Xu Z, Zhou G, Lin H, Zhou J, Zeng Q, Mao Z, Gu X (2015b) Molecular characterization and expression analysis of five chitinases associated with molting in the Chinese mitten crab, Eriocheir sinensis. Comp Biochem Physiol B 187:110–120
Lombard V, Ramulu HG, Drula E et al (2014) The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res 42:D490–D495
Lu Y, Zen KC, Muthukrishnan S, Kramer KJ (2002) Site-directed mutagenesis and functional analysis of active site acidic amino acid residues D142, D144 and E146 in Manduca sexta (tobacco hornworm) chitinase. Insect Biochem Mol Biol 32:1369–1382
Mansur JF, Alvarenga ES, Figueira-Mansur J et al (2014) Effects of chitin synthase double-stranded RNA on molting and oogenesis in the Chagas disease vector Rhodnius prolixus. Insect Biochem Mol Biol 51:110–121
Meng H, Wang Z, Meng X, Xie L, Hunag B (2015) Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea. Genet Mol Biol 38:381–389
Merzendorfer H (2006) Insect chitin synthases: a review. J Comp Physiol 176:1–15
Merzendorfer H (2011) The cellular basis of chitin synthesis in fungi and insects: common principles and differences. Eur J Cell Biol 90:759–769
Merzendorfer H, Zimoch L (2003) Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol 206:4393–4412
Morgan JL, Strumillo J, Zimmer J (2013) Crystallographic snapshot of cellulose synthesis and membrane translocation. Nature 493:181–186
Moussian B (2008) The role of GlcNAc in formation and function of extracellular matrices. Comp Biochem Physiol B Biochem Mol Biol 149:215–226
Moussian B (2010) Recent advances in understanding mechanisms of insect cuticle differentiation. Insect Biochem Mol Biol 40:363–375
Moussian B, Schwarz H, Bartoszewski S et al (2005) Involvement of chitin in exoskeleton morphogenesis in Drosophila melanogaster. J Morphol 264:117–130
Muthukrishnan S, Merzendorfer H, Arakane Y, Kramer KJ (2012) Chitin metabolism in insects. In: Insect molecular biology and biochemistry. Elsevier, London, pp 193–235. doi:10.1016/B978-0-12-384747-8.10007-8
Muthukrishnan S, Merzendorfer H, Arakane Y et al (2016) Chitin metabolic pathways in insects and their regulation. In: Extracellular composite matrices in arthropods. Springer, Cham, pp 31–65
Nakabachi A, Shigenobu S, Miyagishima S (2010) Chitinase-like proteins encoded in the genome of the pea aphid, Acyrthosiphon pisum. Insect Mol Biol 19:175–185
Osman GH, Assem SK, Alreedy RM, El-Ghareeb DK, Basry MA, Rastogi A, Kalaji HM (2015) Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm Spodoptera littoralis. Sci Rep 5:18067
Ostrowski S, Dierick HA, Bejsovec A (2002) Genetic control of cuticle formation during embryonic development of Drosophila melanogaster. Genetics 161:171–182
Pan Y, Lü P, Wang Y, Yin L, Ma H, Ma G, Chen K, He Y (2012) In silico identification of novel chitinase-like proteins in the silkworm, Bombyx mori, genome. J Insect Sci 12:150–163
Pesch YY, Riedel D, Patil KR, Loch G, Behr M (2016) Chitinases and imaginal disc growth factors organize the extracellular matrix formation at barrier tissues in insects. Sci Rep 6:18340
Pillai C, Paul W, Sharma CP (2009) Chitin and chitosan polymers: chemistry, solubility and fiber formation. Prog Polym Sci 34:641–678
Reddy KRK, Rajam MV (2016) Targeting chitinase gene of Helicoverpa armigera by host-induced RNA interference confers insect resistance in tobacco and tomato. Plant Mol Biol 90:281–292
Resch Y, Blatt K, Malkus U, Fercher C, Swoboda I, Focke-Tejkl M, Chen KW, Seiberler S, Mittermann I, Lupinek C, Rodriguez-Dominguez A, Zieglmayer P, Zieglmayer R, Keller W, Krzyzanek V, Valent P, Valenta R, Vrtala S (2016) Molecular, structural and immunological characterization of Der p18, a chitinase-like house dust mite allergen. PLoS One 11:1–19
Reynolds SE (1987) The cuticle, growth and moulting in insects: the essential background to the action of acylurea insecticides. Pestic Sci 20:131–146
Royer V, Fraichard S, Bouhin H (2002) A novel putative insect chitinase with multiple catalytic domains: hormonal regulation during metamorphosis. Biochem J 366:921–928
Ruiz-Herrera J, San-Blas G (2003) Chitin synthesis as a target for antifungal drugs. Curr Drug Targets Infect Disord 3:77–91
Samuels RI, Reynolds SE (1993) Moulting fluid enzymes of the tobacco hornworm, Manduca sexta: timing of proteolytic and chitinolytic activity in relation to pre-ecdysial development. Arch Insect Biochem Physiol 24:33–44
Saxena IM, Brown RM Jr, Fevre M et al (1995) Multidomain architecture of beta-glycosyl transferases: implications for mechanism of action. J Bacteriol 177:1419
Shen Z, Jacobs-Lorena M (1997) Characterization of a novel gut-specific chitinase gene from the human malaria vector Anopheles gambiae. J Biol Chem 272:28895–28900
Soltani N, Chebira S, Delbecque J, Delachambre J (1993) Biological activity of flucycloxuron, a novel benzoylphenylurea derivative, on Tenebrio molitor: comparison with diflubenzuron and triflumuron. Experientia 49:1088–1091
Stern R, Jedrzejas MJ (2008) Carbohydrate polymers at the center of life’s origins: the importance of molecular processivity. Chem Rev 108:5061–5085
Suzuki K, Taiyoji M, Sugawara N, Nikaidou N, Hernissat B, Watanabe T (1999) The third chitinase gene (ChiC) of Serratia marcescens 2170 and the relationship of its product to other bacterial chitinases. Biochem J 343:587–596
Tellam RL (1996) Protein motifs in filarial chitinases: an alternative view. Parasitol Today 12:291–292
Tellam RL, Eisemann C (2000) Chitin is only a minor component of the peritrophic matrix from larvae of Lucilia cuprina. Insect Biochem Mol Biol 30:1189–1201
Tellam RL, Vuocolo T, Johnson SE, Jarmey J, Pearson RD (2000) Insect chitin synthase. Eur J Biochem 267:6025–6043
Tetreau G, Cao X, Chen Y-R, Muthukrishnan S, Jiang H et al (2015) Overview of chitin metabolism enzymes in Manduca sexta: identification, domain organization, phylogenetic analysis and gene expression. Insect Biochem Mol Biol 62:114–126
Tharanathan RN, Kittur FS (2003) Chitin – the undisputed biomolecule of great potential. Crit Rev Food Sci Nutr 43:61–87
Van Leeuwen T, Demaeght P, Osborne EJ et al (2012) Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropods. Proc Natl Acad Sci 109:4407–4412
Veronico P, Gray L, Jones J et al (2001) Nematode chitin synthases: gene structure, expression and function in Caenorhabditis elegans and the plant parasitic nematode Meloidogyne artiellia. Mol Gen Genomics 266:28–34
Wilson TG, Cryan JR (1997) Lufenuron, a chitin synthesis inhibitor, interrupts development of Drosophila melanogaster. J Exp Zool 278:37–44
Wittkopp PJ, Beldade P (2009) Development and evolution of insect pigmentation: genetic mechanisms and the potential consequences of pleiotropy. Semin Cell Dev Biol (A Special Edition on Biosensors and Development of Pigment Cells and Pigment Patterns). Elsevier 20:65–71
Wu Q, Liu T, Yang Q (2013) Cloning, expression and biocharacterization of OfCht5, the chitinase from the insect Ostrinia furnacalis. Insect Sci 20:147–157
Yang B, Zhang M, Li L, Pu F, You W, Ke C (2015) Molecular analysis of atypical family 18 chitinase from fujian Oyster crassostrea angulata and its physiological role in the digestive system. PLoS One 10(6):1–13
Yang M, Wang Y, Jiang F et al (2016) miR-71 and miR-263 jointly regulate target genes chitin synthase and chitinase to control Locust molting. PLoS Genet 12:e1006257
Yeager AR, Finney NS (2004) The first direct evaluation of the two-active site mechanism for chitin synthase. J Organomet Chem 69:613–618
Zechel DL, Withers SG (2000) Glycosidase mechanisms: anatomy of a finely tuned catalyst. Acc Chem Res 33:11–18
Zhang X, Yan Zhu K (2013) Biochemical characterization of chitin synthase activity and inhibition in the African malaria mosquito, Anopheles gambiae. Insect Sci 20:158–166
Zhang X, Zhang J, Zhu K (2010) Chitosan/double stranded RNA nanoparticle mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito (Anopheles gambiae). Insect Mol Biol 19:683–693
Zhang J, Zhang X, Arakane Y, Muthukrishnan S, Kramer KJ, Ma E, Zhu KY (2011) Comparative genomic analysis of chitinase and chitinase-like genes in the African malaria mosquito (Anopheles gambiae). PLoS One 6:e19899
Zhang D, Chen J, Yao Q, Pan Z, Chen J, Zhang W (2012) Functional analysis of two chitinase genes during the pupation and eclosion stages of the beet armyworm Spodoptera exigua by RNA interference. Arch Insect Biochem Physiol 79:220–234
Zhu Q, Deng Y, Vanka P, Brown SJ, Muthukrishnan S et al (2004) Computational identification of novel chitinase-like proteins in the Drosophila melanogaster genome. Bioinformatics 20:161–169
Zhu Q, Arakane Y, Beeman RW, Kramer KJ, Muthukrishnan S (2008a) Characterization of recombinant chitinase-like proteins of Drosophila melanogaster and Tribolium castaneum. Insect Biochem Mol Biol 38:467–477
Zhu Q, Arakane Y, Beeman RW, Kramer KJ, Muthukrishnan S (2008b) Functional specialization among insect chitinase family genes revealed by RNA interference. Proc Natl Acad Sci 105:6650–6655
Zhu Q, Arakane Y, Banerjee D, Beeman RW, Kramer KJ, Muthukrishnan S (2008c) Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects. Insect Biochem Mol Biol 38:452–466
Zhu KY, Merzendorfer H, Zhang W et al (2016) Biosynthesis, turnover, and functions of chitin in insects. Annu Rev Entomol 61:177–196
Zhuo W, Fang Y, Kong L et al (2014) Chitin synthase A: a novel epidermal development regulation gene in the larvae of Bombyx mori. Mol Biol Rep 41:4177–4186
Zimoch L, Hogenkamp D, Kramer K, Muthukrishnan S, Merzendorfer H (2005) Regulation of chitin synthesis in the larval midgut of Manduca sexta. Insect Biochem Mol Biol 35:515–527
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Kumar, M., Vivekanand, V., Pareek, N. (2018). Structure, Regulation, and Potential Applications of Insect Chitin-Metabolizing Enzymes. In: Kumar, D., Gong, C. (eds) Trends in Insect Molecular Biology and Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-319-61343-7_14
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