Abstract
Luteinization is a natural process in bovine, including other mammals, which is indicated by lipid accumulation in small and big granulosa cell. AMPK is one of the major genes controlling this process, which is also involved in the regulation of lipogenesis and its accumulation into granulosa cells. MicroRNAs (miRNAs) are a class of small (19–24 nt) noncoding regulatory RNA that silences gene expression posttranscriptionally through the recognition of complementary sequences between miRNAs and their 3-UTR target genes. Potential miRNAs which may target AMPKA1, catalytic subunit alpha (α) of AMPK heterotrimeric complex, were identified using four miRNA target prediction databases such as miRanda, miRDB, PICTAR, and TargetScan and resulted 33, 28, 16, and 29 predicted miRNAs, respectively. Among them five miRNAs, namely, miR-301, miR-130, miR-101, miR-19a, and miR-19b, were found to be common in miRNAs predicted by four target prediction tools. The sequence similarity and alignment of these miRNAs in human (hsa) and bovine (bta) were determined using miRBase and found that four miRNAs were 100% identical, and only miR-301a has 92% similarity; therefore miR-301a is excluded from the listed miRNA candidate. Importantly, we also found that miR-19b, miR-130, miR-101, and miR-19a have 3, 7, 4, and 3 binding sites, respectively, at 3′ UTR region of AMPKA1. Further experiments focused on candidate miRNA profiling in luteinized granulosa cells, and target validation study is required in order to validate the results of this in silico study.
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Prastowo, S., Amin, A., Sohel, M.H. (2017). Identifying Candidate MicroRNAs in MicroRNA-AMPK Gene Interaction Regulating Lipid Accumulation of Bovine Granulosa Cell Luteinization: An In Silico Study. In: Isnansetyo, A., Nuringtyas, T. (eds) Proceeding of the 1st International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-60363-6_45
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