Sperm Quality of Gembrong Goat in Bali Before and After Freezing
This research aims to study the sperm characteristics of Gembrong goat in Bali before and after freezing, using citrate-egg yolk (CEY) and tris-egg yolk (TEY) diluents. The sperm of six goats was collected using an artificial vagina. The results showed that there were no significant differences between the use of CEY and TEY diluents on motility, viability, and abnormalities of spermatozoa neither before nor after freezing. Before freezing, motility was CEY 85 ± 3.5, TEY 85 ± 2.1; viability was CEY 90 ± 3.5, TEY 92 ± 2.1; and abnormalities were CEY 11 ± 3.5, TEY 10 ± 2, 1, while after freezing, motility was CEY 45 ± 3.5 b, TEY 46 ± 4.1; viability was CEY 70 ± 3.5, TEY 72 ± 4.1; and abnormalities were CEY 15 ± 3.5, TEY 16 ± 4.1. However, the freezing process significantly (p < 0.01) lowered sperm quality in terms of motility, viability, and abnormalities, both for CEY and TEY diluents. In CEY diluent, motility before was 85 ± 3.5 and after was 45 ± 3.5, viability before was 90 ± 3.5 and after was 70 ± 3.5, and abnormalities before were 11 ± 3.5 and after were 15 ± 3.5. In TEY diluent, motility before was 85 ± 2.1 and after was 46 ± 4.1, viability before was 92 ± 2.1 and after was 72 ± 4.1, and abnormalities before were 10 ± 2.1 and after were 16 ± 4.1. Dilution and freezing of Gembrong goat sperm using citrate-egg yolk and tris-egg yolk showed no significant differences, but the freezing process caused a decline in sperm quality.
KeywordsCitrate-egg yolk Gembrong goat Sperm characteristics Tris-egg yolk
Thanks and high appreciations are due to the Directorate General of Higher Education which has funded this research, and Institute for Agricultural Technology, Bali Province, and Agency for Animal Husbandry, Karangasem District, which have facilitated this research (130/LPPM/2015, 2 Maret 2015).
- 1.Sidadolog J.H.P., et al.: Studi karakteristik biologis Kambing Gembrong sebagai plasma nutfah Indonesia [Study of goats Gembrong biological characteristics as germplasm Indonesia]. Research Report of Animal Science Faculty Universitas Gadjah Mada. Yogyakarta (2012) (in Bahasa Indonesia)Google Scholar
- 2.Glenn W.S., Vandemark N.I.: Physiology of Reproduction and Artificial Insemination of Cattle. Indonesian edition: Glenn W.S., Vandemark N.I.: Fisiologi Reproduksi dan Inseminasi Buatan pada Sapi. (Trans: R. Januar). Universitas Gadjah Madaa Press. Yogyakarta (1985)Google Scholar
- 4.Apu, A.S., Yahia Kandhoker, M.A.M., Husain, S.S., Fakruzzaman, M., Notter, D.R.: A comparative study of fresh and frozen-thawed semen quality in relation to fertility of Black Bengal Goats. Irania J. Appl. Anim. Sci. 2, 157–161 (2015)Google Scholar
- 6.Bintara S.: Peningkatan kinerja reproduksi induk kambing Bligon melalui seleksi pejantan, identifikasi dan separasi spermatozoa, serta suplementasi energi-protein [Reproductive performance improvement on Bligon doe through buck selection, identification and separation of spermatozoa, and energy-protein supplementation]. Disertation. Universitas Gadjah Mada, Yogyakarta (2009) (in Bahasa Indonesia)Google Scholar
- 7.Bearden, H.J., Fuquay, J.W., Willard, S.T.: Applied Animal Reproduction, 6th edn. Pearson Prentice Hall, Upper Saddle River (2004)Google Scholar
- 8.Aguiar G.V., et al.: Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen. Arq. Bras. Med. Vet. Zootec. 65(1) Belo Horizonte (http://www.scielo.br/scielo.php?script=sci_arttext&pid= S0102-09352013000100002) (2013)
- 9.Susilawati, T.: Spermatology. Universitas Brawijaya Press, Malang (2011)Google Scholar