The aim of this study was to identify the position of specific restriction enzyme in mtDNA cytochrome b gene on Angus grade cattle. Angus grade cattle were the result of crossbreeding between Indonesian local cattle and Angus cattle in Sragen, Central Java, Indonesia. Restriction enzyme mapping on mtDNA cytochrome b gene was carried on Angus grade cattle (n = 15), and genetic comparisons were done for cattle GenBank (n = 54) from various breeds and countries. Cytochrome b gene was amplified using a pair of specific primer forward and reverse, resulting 464 bp of amplification product. Bioedit was used for sequence analysis and chosen restriction enzymes with 4-bases and 6-bases for mapping restriction site. The results showed 46 SNPs, which can be grouped into nine genotypes, and Angus grade cattle can be divided into Types 1 and 2. The nucleotide sequence was truncated by 23 restriction enzymes, but only 10 restriction enzymes (six enzyme 4-bases, four enzyme 6-bases) were recommended to analyze the cytochrome b gene on Angus grade cattle, such as AciI, AluI, HaeIII, HpyCH4V, TaqI, Tsp509I, BfrBI, NsiI, StuI, and XbaI. Angus grade cattle had genetic diversity based on the results of restriction enzyme mapping on cytochrome b gene.
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Acknowledgments are addressed to Grati Beef Cattle Research Station (Loka Penelitian Sapi Potong Grati) for the cooperation and assistance in the collection of the blood samples; we also thank Retno Setyawati for helping in the DNA extraction. The research was partly supported by Faculty of Animal Science, Graduate Research Grants year 2016.
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